Reviewed, UniProtKB/Swiss-Prot O94832 (MYO1D_HUMAN)

Names and origin

Protein names

Recommended name:
    Myosin-Id

Gene names

Name:	MYO1D
Synonyms:	KIAA0727

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments By similarity.
Subunit structure	Binds calmodulin through its IQ motifs By similarity.
Tissue specificity	Expressed in many tissues. Highest levels in brain, followed by lung and ovary; expression is lowest in spleen.
Sequence similarities	Contains 2 IQ domains. Contains 1 myosin head-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	ATP-binding Actin-binding Calmodulin-binding Nucleotide-binding
Molecular function	Motor protein Myosin
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	myosin complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW actin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW motor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ILK	Q13418	1	EBI-355634,EBI-747644
PELO	Q9BRX2	1	EBI-355634,EBI-1043580

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94832-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94832-2)

The sequence of this isoform differs from the canonical sequence as follows:
     433-436: IDYF → VGLL
     437-1006: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 1006	1005	Myosin-Id		PRO_0000123447
Regions
	Domain	2 – 682	681	Myosin head-like
	Domain	699 – 719	21	IQ 1
	Domain	721 – 741	21	IQ 2
	Nucleotide binding	102 – 109	8	ATP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.4
	Modified residue	200	1	Phosphoserine Ref.5
	Modified residue	203	1	Phosphoserine Ref.5
	Modified residue	212	1	Phosphoserine Ref.5
	Modified residue	536	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	433 – 436	4	IDYF → VGLL in isoform 2.		VSP_007631
	Alternative sequence	437 – 1006	570	Missing in isoform 2.		VSP_007632
	Natural variant	765	1	P → S: dbSNP rs7209106.		VAR_050210
	Natural variant	771	1	R → H: dbSNP rs7215958.		VAR_050211

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 20, 2003. Version 2. Checksum: A8BB08450887168E	FASTA	1,006	116,202
	10 20 30 40 50 60 MAEQESLEFG KADFVLMDTV SMPEFMANLR LRFEKGRIYT FIGEVVVSVN PYKLLNIYGR 70 80 90 100 110 120 DTIEQYKGRE LYERPPHLFA IADAAYKAMK RRSKDTCIVI SGESGAGKTE ASKYIMQYIA 130 140 150 160 170 180 AITNPSQRAE VERVKNMLLK SNCVLEAFGN AKTNRNDNSS RFGKYMDINF DFKGDPIGGH 190 200 210 220 230 240 INNYLLEKSR VIVQQPGERS FHSFYQLLQG GSEQMLRSLH LQKSLSSYNY IHVGAQLKSS 250 260 270 280 290 300 INDAAEFRVV ADAMKVIGFK PEEIQTVYKI LAAILHLGNL KFVVDGDTPL IENGKVVSII 310 320 330 340 350 360 AELLSTKTDM VEKALLYRTV ATGRDIIDKQ HTEQEASYGR DAFAKAIYER LFCWIVTRIN 370 380 390 400 410 420 DIIEVKNYDT TIHGKNTVIG VLDIYGFEIF DNNSFEQFCI NYCNEKLQQL FIQLVLKQEQ 430 440 450 460 470 480 EEYQREGIPW KHIDYFNNQI IVDLVEQQHK GIIAILDDAC MNVGKVTDEM FLEALNSKLG 490 500 510 520 530 540 KHAHFSSRKL CASDKILEFD RDFRIRHYAG DVVYSVIGFI DKNKDTLFQD FKRLMYNSSN 550 560 570 580 590 600 PVLKNMWPEG KLSITEVTKR PLTAATLFKN SMIALVDNLA SKEPYYVRCI KPNDKKSPQI 610 620 630 640 650 660 FDDERCRHQV EYLGLLENVR VRRAGFAFRQ TYEKFLHRYK MISEFTWPNH DLPSDKEAVK 670 680 690 700 710 720 KLIERCGFQD DVAYGKTKIF IRTPRTLFTL EELRAQMLIR IVLFLQKVWR GTLARMRYKR 730 740 750 760 770 780 TKAALTIIRY YRRYKVKSYI HEVARRFHGV KTMRDYGKHV KWPSPPKVLR RFEEALQTIF 790 800 810 820 830 840 NRWRASQLIK SIPASDLPQV RAKVAAVEML KGQRADLGLQ RAWEGNYLAS KPDTPQTSGT 850 860 870 880 890 900 FVPVANELKR KDKYMNVLFS CHVRKVNRFS KVEDRAIFVT DRHLYKMDPT KQYKVMKTIP 910 920 930 940 950 960 LYNLTGLSVS NGKDQLVVFH TKDNKDLIVC LFSKQPTHES RIGELVGVLV NHFKSEKRHL 970 980 990 1000 QVNVTNPVQC SLHGKKCTVS VETRLNQPQP DFTKNRSGFI LSVPGN
	Isoform 2. Checksum: 1FEBDA4532DE0176	FASTA	436	49,769
	10 20 30 40 50 60 MAEQESLEFG KADFVLMDTV SMPEFMANLR LRFEKGRIYT FIGEVVVSVN PYKLLNIYGR 70 80 90 100 110 120 DTIEQYKGRE LYERPPHLFA IADAAYKAMK RRSKDTCIVI SGESGAGKTE ASKYIMQYIA 130 140 150 160 170 180 AITNPSQRAE VERVKNMLLK SNCVLEAFGN AKTNRNDNSS RFGKYMDINF DFKGDPIGGH 190 200 210 220 230 240 INNYLLEKSR VIVQQPGERS FHSFYQLLQG GSEQMLRSLH LQKSLSSYNY IHVGAQLKSS 250 260 270 280 290 300 INDAAEFRVV ADAMKVIGFK PEEIQTVYKI LAAILHLGNL KFVVDGDTPL IENGKVVSII 310 320 330 340 350 360 AELLSTKTDM VEKALLYRTV ATGRDIIDKQ HTEQEASYGR DAFAKAIYER LFCWIVTRIN 370 380 390 400 410 420 DIIEVKNYDT TIHGKNTVIG VLDIYGFEIF DNNSFEQFCI NYCNEKLQQL FIQLVLKQEQ 430 EEYQREGIPW KHVGLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Nagase T., Kikuno R., Yamakawa H., Ohara O. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-1006 (ISOFORM 1). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[4]	Bienvenut W.V., Dozynkiewicz M., Norman J.C. Submitted (MAR-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-11; 239-248; 534-544; 791-801 AND 985-994, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200; SER-203 AND SER-212, MASS SPECTROMETRY.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB018270 mRNA. Translation: BAA34447.2. Different initiation. BC030602 mRNA. Translation: AAH30602.1. BC146763 mRNA. Translation: AAI46764.1.
IPI	IPI00329719. IPI00412487.
RefSeq	NP_056009.1.
UniGene	Hs.658000
3D structure databases
HSSP	HSSP built from PDB template 1MND based on UniProtKB P08799.
ModBase	Search...
Protein-protein interaction databases
IntAct	O94832. 12 interactions.
PTM databases
PhosphoSite	O94832.
Proteomic databases
PeptideAtlas	O94832.
PRIDE	O94832.
Genome annotation databases
Ensembl	ENSG00000176658. Homo sapiens. [Contig view]
GeneID	4642.
KEGG	hsa:4642.
UCSC	uc002hho.1. human.
Organism-specific databases
GeneCards	GC17M027844.
H-InvDB	HIX0013706.
HGNC	HGNC:7598. MYO1D.
MIM	606539. gene.
PharmGKB	PA31400.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O94832.
HOVERGEN	O94832.
OMA	O94832. YIHEVAR.
Gene expression databases
ArrayExpress	O94832.
Bgee	O94832.
CleanEx	HS_MYO1D.
GermOnline	ENSG00000176658. Homo sapiens.
Family and domain databases
InterPro	IPR000048. IQ_CaM_bd_region. IPR001609. Myosin_head. IPR010926. Myosin_tail_2. [Graphical view]
Pfam	PF00612. IQ. 1 hit. PF00063. Myosin_head. 1 hit. PF06017. Myosin_TH1. 1 hit. [Graphical view]
PRINTS	PR00193. MYOSINHEAVY.
ProDom	PD000355. Myosin_head. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00015. IQ. 1 hit. SM00242. MYSc. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	17888.
SOURCE	Search...

Entry information

Entry name

MYO1D_HUMAN

Accession

Primary (citable) accession number: O94832
Secondary accession number(s): A6H8V3, Q8NHP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 20, 2003
Last modified:	July 7, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)