Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O94829 (IPO13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Importin-13

Short name=Imp13
Alternative name(s):
Karyopherin-13
Short name=Kap13
Ran-binding protein 13
Short name=RanBP13
Gene names
Name:IPO13
Synonyms:KIAA0724, RANBP13
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length963 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity. Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. Ref.1 Ref.8

Subunit structure

Interacts with UBC9, RAN, RBM8A, eIF-1A and PAX6. Ref.1 Ref.8

Subcellular location

Cytoplasm. Nucleus.

Tissue specificity

Expressed in fetal brain, heart, intestine and kidney. Ref.9

Sequence similarities

Belongs to the importin beta family.

Contains 20 HEAT repeats.

Contains 1 importin N-terminal domain.

Sequence caution

The sequence BAA34444.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAB14575.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein import into nucleus

Inferred from direct assay PubMed 15964792. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 16189514Ref.12. Source: IntAct

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 963963Importin-13
PRO_0000120758

Regions

Repeat24 – 5431HEAT 1
Domain45 – 11167Importin N-terminal
Repeat56 – 8833HEAT 2
Repeat95 – 13541HEAT 3
Repeat142 – 17938HEAT 4
Repeat194 – 23138HEAT 5
Repeat236 – 26833HEAT 6
Repeat276 – 32550HEAT 7
Repeat330 – 37243HEAT 8
Repeat375 – 43864HEAT 9
Repeat440 – 47637HEAT 10
Repeat487 – 52236HEAT 11
Repeat524 – 55835HEAT 12
Repeat562 – 60039HEAT 13
Repeat603 – 64846HEAT 14
Repeat676 – 71641HEAT 15
Repeat720 – 75435HEAT 16
Repeat761 – 80343HEAT 17
Repeat815 – 84531HEAT 18
Repeat860 – 89334HEAT 19
Repeat897 – 93135HEAT 20

Experimental info

Sequence conflict2021F → V in CAB55966. Ref.6
Sequence conflict7151T → A in BAB14575. Ref.7
Sequence conflict8171A → S in AAH08194. Ref.5

Secondary structure

............................................................................................................................... 963
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O94829 [UniParc].

Last modified July 19, 2004. Version 3.
Checksum: 8C696EE2B92365F0

FASTA963108,195
        10         20         30         40         50         60 
MERREEQPGA AGAGAAPALD FTVENVEKAL HQLYYDPNIE NKNLAQKWLM QAQVSPQAWH 

        70         80         90        100        110        120 
FSWQLLQPDK VPEIQYFGAS ALHIKISRYW SDIPTDQYES LKAQLFTQIT RFASGSKIVL 

       130        140        150        160        170        180 
TRLCVALASL ALSMMPDAWP CAVADMVRLF QAEDSPVDGQ GRCLALLELL TVLPEEFQTS 

       190        200        210        220        230        240 
RLPQYRKGLV RTSLAVECGA VFPLLEQLLQ QPSSPSCVRQ KVLKCFSSWV QLEVPLQDCE 

       250        260        270        280        290        300 
ALIQAAFAAL QDSELFDSSV EAIVNAISQP DAQRYVNTLL KLIPLVLGLQ EQLRQAVQNG 

       310        320        330        340        350        360 
DMETSHGICR IAVALGENHS RALLDQVEHW QSFLALVNMI MFCTGIPGHY PVNETTSSLT 

       370        380        390        400        410        420 
LTFWYTLQDD ILSFEAEKQA VYQQVYRPVY FQLVDVLLHK AQFPSDEEYG FWSSDEKEQF 

       430        440        450        460        470        480 
RIYRVDISDT LMYVYEMLGA ELLSNLYDKL GRLLTSSEEP YSWQHTEALL YGFQSIAETI 

       490        500        510        520        530        540 
DVNYSDVVPG LIGLIPRISI SNVQLADTVM FTIGALSEWL ADHPVMINSV LPLVLHALGN 

       550        560        570        580        590        600 
PELSVSSVST LKKICRECKY DLPPYAANIV AVSQDVLMKQ IHKTSQCMWL MQALGFLLSA 

       610        620        630        640        650        660 
LQVEEILKNL HSLISPYIQQ LEKLAEEIPN PSNKLAIVHI LGLLSNLFTT LDISHHEDDH 

       670        680        690        700        710        720 
EGPELRKLPV PQGPNPVVVV LQQVFQLIQK VLSKWLNDAQ VVEAVCAIFE KSVKTLLDDF 

       730        740        750        760        770        780 
APMVPQLCEM LGRMYSTIPQ ASALDLTRQL VHIFAHEPAH FPPIEALFLL VTSVTLTLFQ 

       790        800        810        820        830        840 
QGPRDHPDIV DSFMQLLAQA LKRKPDLFLC ERLDVKAVFQ CAVLALKFPE APTVKASCGF 

       850        860        870        880        890        900 
FTELLPRCGE VESVGKVVQE DGRMLLIAVL EAIGGQASRS LMDCFADILF ALNKHCFSLL 

       910        920        930        940        950        960 
SMWIKEALQP PGFPSARLSP EQKDTFSQQI LRERVNKRRV KEMVKEFTLL CRGLHGTDYT 


ADY 

« Hide

References

« Hide 'large scale' references
[1]"Importin 13: a novel mediator of nuclear import and export."
Mingot J.-M., Kostka S., Kraft R., Hartmann E., Goerlich D.
EMBO J. 20:3685-3694(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RBM8A; UBC9 AND EIF1A.
[2]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cervix.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-963.
Tissue: Testis.
[7]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 495-963.
Tissue: Placenta.
[8]"Paired-type homeodomain transcription factors are imported into the nucleus by karyopherin 13."
Ploski J.E., Shamsher M.K., Radu A.
Mol. Cell. Biol. 24:4824-4834(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PAX6.
[9]"A novel karyopherin-beta homolog is developmentally and hormonally regulated in fetal lung."
Zhang C., Sweezey N.B., Gagnon S., Muskat B., Koehler D., Post M., Kaplan F.
Am. J. Respir. Cell Mol. Biol. 22:451-459(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Nuclear import mechanism of the EJC component Mago-Y14 revealed by structural studies of importin 13."
Bono F., Cook A.G., Grunwald M., Ebert J., Conti E.
Mol. Cell 37:211-222(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH YEAST GSP1/RAN, REPEAT STRUCTURE.
[12]"Structure of Importin13-Ubc9 complex: nuclear import and release of a key regulator of sumoylation."
Grunwald M., Bono F.
EMBO J. 30:427-438(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH UBE2I/UBC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF267987 mRNA. Translation: AAF73471.1.
AB018267 mRNA. Translation: BAA34444.2. Different initiation.
AL357079 Genomic DNA. Translation: CAI16798.1.
CH471059 Genomic DNA. Translation: EAX07072.1.
CH471059 Genomic DNA. Translation: EAX07073.1.
BC008194 mRNA. Translation: AAH08194.1.
AL117501 mRNA. Translation: CAB55966.1.
AK023441 mRNA. Translation: BAB14575.1. Different initiation.
CCDSCCDS503.1.
PIRT17276.
RefSeqNP_055467.3. NM_014652.3.
UniGeneHs.158497.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2X19X-ray2.80B1-963[»]
2XWUX-ray2.80B1-963[»]
3ZJYX-ray3.60B/E/G1-963[»]
ProteinModelPortalO94829.
SMRO94829. Positions 19-953.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115025. 20 interactions.
IntActO94829. 9 interactions.
MINTMINT-1465082.
STRING9606.ENSP00000361418.

PTM databases

PhosphoSiteO94829.

Proteomic databases

MaxQBO94829.
PaxDbO94829.
PRIDEO94829.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000372343; ENSP00000361418; ENSG00000117408.
GeneID9670.
KEGGhsa:9670.
UCSCuc001ckx.3. human.

Organism-specific databases

CTD9670.
GeneCardsGC01P044412.
HGNCHGNC:16853. IPO13.
MIM610411. gene.
neXtProtNX_O94829.
PharmGKBPA134981096.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG237172.
HOVERGENHBG052141.
InParanoidO94829.
OMAQMYSTIP.
OrthoDBEOG718KC9.
PhylomeDBO94829.
TreeFamTF314539.

Gene expression databases

ArrayExpressO94829.
BgeeO94829.
CleanExHS_IPO13.
GenevestigatorO94829.

Family and domain databases

Gene3D1.25.10.10. 3 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view]
PfamPF03810. IBN_N. 1 hit.
PF08389. Xpo1. 1 hit.
[Graphical view]
SMARTSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
PROSITEPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIPO13. human.
EvolutionaryTraceO94829.
GeneWikiIPO13.
GenomeRNAi9670.
NextBio36311.
PROO94829.
SOURCESearch...

Entry information

Entry nameIPO13_HUMAN
AccessionPrimary (citable) accession number: O94829
Secondary accession number(s): D3DPY4 expand/collapse secondary AC list , Q5T4X3, Q7LC04, Q96HS3, Q9H8N3, Q9UFR1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM