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O94823

- AT10B_HUMAN

UniProt

O94823 - AT10B_HUMAN

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Protein
Probable phospholipid-transporting ATPase VB
Gene
ATP10B, ATPVB, KIAA0715
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules Inferred.

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei433 – 43314-aspartylphosphate intermediate By similarity
Metal bindingi1055 – 10551Magnesium By similarity
Metal bindingi1059 – 10591Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cation-transporting ATPase activity Source: InterPro
  3. magnesium ion binding Source: InterPro
  4. phospholipid-translocating ATPase activity Source: UniProtKB-EC
  5. protein binding Source: UniProtKB

GO - Biological processi

  1. phospholipid translocation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_25149. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase VB (EC:3.6.3.1)
Alternative name(s):
ATPase class V type 10B
P4-ATPase flippase complex alpha subunit ATP10B
Gene namesi
Name:ATP10B
Synonyms:ATPVB, KIAA0715
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:13543. ATP10B.

Subcellular locationi

Cytoplasmic vesicle membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane
Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8282Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei83 – 10422Helical; Reviewed prediction
Add
BLAST
Topological domaini105 – 1106Exoplasmic loop Reviewed prediction
Transmembranei111 – 13222Helical; Reviewed prediction
Add
BLAST
Topological domaini133 – 316184Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei317 – 33822Helical; Reviewed prediction
Add
BLAST
Topological domaini339 – 36830Exoplasmic loop Reviewed prediction
Add
BLAST
Transmembranei369 – 39022Helical; Reviewed prediction
Add
BLAST
Topological domaini391 – 1111721Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1112 – 113221Helical; Reviewed prediction
Add
BLAST
Topological domaini1133 – 114412Exoplasmic loop Reviewed prediction
Add
BLAST
Transmembranei1145 – 116420Helical; Reviewed prediction
Add
BLAST
Topological domaini1165 – 119430Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1195 – 121622Helical; Reviewed prediction
Add
BLAST
Topological domaini1217 – 12237Exoplasmic loop Reviewed prediction
Transmembranei1224 – 124623Helical; Reviewed prediction
Add
BLAST
Topological domaini1247 – 12526Cytoplasmic Reviewed prediction
Transmembranei1253 – 127321Helical; Reviewed prediction
Add
BLAST
Topological domaini1274 – 129118Exoplasmic loop Reviewed prediction
Add
BLAST
Transmembranei1292 – 131625Helical; Reviewed prediction
Add
BLAST
Topological domaini1317 – 1461145Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  2. endoplasmic reticulum Source: UniProtKB
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25098.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14611461Probable phospholipid-transporting ATPase VB
PRO_0000046381Add
BLAST

Proteomic databases

PaxDbiO94823.
PRIDEiO94823.

PTM databases

PhosphoSiteiO94823.

Expressioni

Tissue specificityi

Found in brain and in low levels in testis.

Gene expression databases

ArrayExpressiO94823.
BgeeiO94823.
CleanExiHS_ATP10B.
GenevestigatoriO94823.

Organism-specific databases

HPAiHPA034574.

Interactioni

Subunit structurei

Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit Inferred.

Protein-protein interaction databases

STRINGi9606.ENSP00000313600.

Structurei

3D structure databases

ProteinModelPortaliO94823.
SMRiO94823. Positions 1029-1089.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000202528.
HOVERGENiHBG107129.
InParanoidiO94823.
OMAiTGFGCES.
OrthoDBiEOG76471S.
PhylomeDBiO94823.
TreeFamiTF354252.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform A (identifier: O94823-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MALSVDSSWH RWQWRVRDGF PHCPSETTPL LSPEKGRQSY NLTQQRVVFP     50
NNSIFHQDWE EVSRRYPGNR TCTTKYTLFT FLPRNLFEQF HRWANLYFLF 100
LVILNWMPSM EVFHREITML PLAIVLFVIM IKDGMEDFKR HRFDKAINCS 150
NIRIYERKEQ TYVQKCWKDV RVGDFIQMKC NEIVPADILL LFSSDPNGIC 200
HLETASLDGE TNLKQRCVVK GFSQQEVQFE PELFHNTIVC EKPNNHLNKF 250
KGYMEHPDQT RTGFGCESLL LRGCTIRNTE MAVGIVIYAG HETKAMLNNS 300
GPRYKRSKIE RRMNIDIFFC IGILILMCLI GAVGHSIWNG TFEEHPPFDV 350
PDANGSFLPS ALGGFYMFLT MIILLQVLIP ISLYVSIELV KLGQVFFLSN 400
DLDLYDEETD LSIQCRALNI AEDLGQIQYI FSDKTGTLTE NKMVFRRCTI 450
MGSEYSHQEN AKRLETPKEL DSDGEEWTQY QCLSFSARWA QDPATMRSQK 500
GAQPLRRSQS ARVPIQGHYR QRSMGHRESS QPPVAFSSSI EKDVTPDKNL 550
LTKVRDAALW LETLSDSRPA KASLSTTSSI ADFFLALTIC NSVMVSTTTE 600
PRQRVTIKPS SKALGTSLEK IQQLFQKLKL LSLSQSFSST APSDTDLGES 650
LGANVATTDS DERDDASVCS GGDSTDDGGY RSSMWDQGDI LESGSGTSLE 700
EALEAPATDL ARPEFCYEAE SPDEAALVHA AHAYSFTLVS RTPEQVTVRL 750
PQGTCLTFSL LCTLGFDSVR KRMSVVVRHP LTGEIVVYTK GADSVIMDLL 800
EDPACVPDIN MEKKLRKIRA RTQKHLDLYA RDGLRTLCIA KKVVSEEDFR 850
RWASFRREAE ASLDNRDELL METAQHLENQ LTLLGATGIE DRLQEGVPDT 900
IATLREAGIQ LWVLTGDKQE TAVNIAHSCR LLNQTDTVYT INTENQETCE 950
SILNCALEEL KQFRELQKPD RKLFGFRLPS KTPSITSEAV VPEAGLVIDG 1000
KTLNAIFQGK LEKKFLELTQ YCRSVLCCRS TPLQKSMIVK LVRDKLRVMT 1050
LSIGDGANDV SMIQAADIGI GISGQEGMQA VMSSDFAITR FKHLKKLLLV 1100
HGHWCYSRLA RMVVYYLYKN VCYVNLLFWY QFFCGFSSST MIDYWQMIFF 1150
NLFFTSLPPL VFGVLDKDIS AETLLALPEL YKSGQNSECY NLSTFWISMV 1200
DAFYQSLICF FIPYLAYKGS DIDVFTFGTP INTISLTTIL LHQAMEMKTW 1250
TIFHGVVLLG SFLMYFLVSL LYNATCVICN SPTNPYWVME GQLSNPTFYL 1300
VCFLTPVVAL LPRYFFLSLQ GTCGKSLISK AQKIDKLPPD KRNLEIQSWR 1350
SRQRPAPVPE VARPTHHPVS SITGQDFSAS TPKSSNPPKR KHVEESVLHE 1400
QRCGTECMRD DSCSGDSSAQ LSSGEHLLGP NRIMAYSRGQ TDMCRCSKRS 1450
SHRRSQSSLT I 1461
Length:1,461
Mass (Da):165,391
Last modified:April 30, 2003 - v2
Checksum:i2676B90416B6B541
GO
Isoform B (identifier: O94823-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     461-529: AKRLETPKEL...RQRSMGHRES → GIEAPKGSIP...TCKLSFVYKG
     530-1461: Missing.

Note: No experimental confirmation available.

Show »
Length:529
Mass (Da):61,243
Checksum:i048EAC8E3D5BBA0D
GO
Isoform C (identifier: O94823-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: MALSVDSSWH...INCSNIRIYE → MKKEGRKRWK...INNRECLIYS
     461-529: AKRLETPKEL...RQRSMGHRES → GIEAPKGSIP...TCKLSFVYKG
     530-1461: Missing.

Note: No experimental confirmation available.

Show »
Length:501
Mass (Da):57,465
Checksum:iD12B13EC2EC2E3D5
GO

Sequence cautioni

The sequence BAA34435.2 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti217 – 2171C → R.1 Publication
Corresponds to variant rs958912 [ dbSNP | Ensembl ].
VAR_048384

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 156156MALSV…IRIYE → MKKEGRKRWKRKEDKKRVVV SNLLFEGWSHKENPNRHHRG NQIKTSKYTVLSFVPKNIFE QLHRFANLYFVGIAVLNFIP VVNAFQPEVSMIPICVILAV TAIKDAWEDLRRYKSDKVIN NRECLIYS in isoform C.
VSP_007305Add
BLAST
Alternative sequencei461 – 52969AKRLE…GHRES → GIEAPKGSIPLSKRKYPALL RNEEIKDILLALLEAVWHFH KLLPVSLWSSLSQIRAVPIT CKLSFVYKG in isoform B and isoform C.
VSP_007306Add
BLAST
Alternative sequencei530 – 1461932Missing in isoform B and isoform C.
VSP_007307Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2341F → S in BAC03528. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018258 mRNA. Translation: BAA34435.2. Different initiation.
AK090832 mRNA. Translation: BAC03528.1.
AK025130 mRNA. Translation: BAB15074.1.
AC008456 Genomic DNA. No translation available.
CCDSiCCDS43394.1. [O94823-1]
RefSeqiNP_079429.2. NM_025153.2. [O94823-1]
XP_006714896.1. XM_006714833.1. [O94823-1]
UniGeneiHs.109358.

Genome annotation databases

EnsembliENST00000327245; ENSP00000313600; ENSG00000118322. [O94823-1]
GeneIDi23120.
KEGGihsa:23120.
UCSCiuc003lym.1. human. [O94823-1]
uc003lyo.2. human. [O94823-3]
uc003lyp.2. human. [O94823-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018258 mRNA. Translation: BAA34435.2 . Different initiation.
AK090832 mRNA. Translation: BAC03528.1 .
AK025130 mRNA. Translation: BAB15074.1 .
AC008456 Genomic DNA. No translation available.
CCDSi CCDS43394.1. [O94823-1 ]
RefSeqi NP_079429.2. NM_025153.2. [O94823-1 ]
XP_006714896.1. XM_006714833.1. [O94823-1 ]
UniGenei Hs.109358.

3D structure databases

ProteinModelPortali O94823.
SMRi O94823. Positions 1029-1089.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000313600.

PTM databases

PhosphoSitei O94823.

Proteomic databases

PaxDbi O94823.
PRIDEi O94823.

Protocols and materials databases

DNASUi 23120.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000327245 ; ENSP00000313600 ; ENSG00000118322 . [O94823-1 ]
GeneIDi 23120.
KEGGi hsa:23120.
UCSCi uc003lym.1. human. [O94823-1 ]
uc003lyo.2. human. [O94823-3 ]
uc003lyp.2. human. [O94823-2 ]

Organism-specific databases

CTDi 23120.
GeneCardsi GC05M159922.
HGNCi HGNC:13543. ATP10B.
HPAi HPA034574.
neXtProti NX_O94823.
PharmGKBi PA25098.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000202528.
HOVERGENi HBG107129.
InParanoidi O94823.
OMAi TGFGCES.
OrthoDBi EOG76471S.
PhylomeDBi O94823.
TreeFami TF354252.

Enzyme and pathway databases

Reactomei REACT_25149. Ion transport by P-type ATPases.

Miscellaneous databases

GenomeRNAii 23120.
NextBioi 44343.
PROi O94823.

Gene expression databases

ArrayExpressi O94823.
Bgeei O94823.
CleanExi HS_ATP10B.
Genevestigatori O94823.

Family and domain databases

Gene3Di 2.70.150.10. 2 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProi IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
PANTHERi PTHR24092. PTHR24092. 1 hit.
Pfami PF00122. E1-E2_ATPase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Tissue: Brain.
  2. Ohara O., Suyama M., Nagase T., Ishikawa K., Kikuno R.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS B AND C), VARIANT ARG-217.
    Tissue: Amygdala and Colon.
  4. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiAT10B_HUMAN
AccessioniPrimary (citable) accession number: O94823
Secondary accession number(s): Q9H725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 30, 2003
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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