ID   ATG12_HUMAN             Reviewed;         140 AA.
AC   O94817; Q6PJV2;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-JAN-2012, entry version 94.
DE   RecName: Full=Ubiquitin-like protein ATG12;
DE   AltName: Full=Autophagy-related protein 12;
DE            Short=APG12-like;
GN   Name=ATG12; Synonyms=APG12, APG12L;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CONJUGATION TO ATG5, AND
RP   TISSUE SPECIFICITY.
RX   MEDLINE=99069367; PubMed=9852036; DOI=10.1074/jbc.273.51.33889;
RA   Mizushima N., Sugita H., Yoshimori T., Ohsumi Y.;
RT   "A new protein conjugation system in human. The counterpart of the
RT   yeast Apg12p conjugation system essential for autophagy.";
RL   J. Biol. Chem. 273:33889-33892(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15372022; DOI=10.1038/nature02919;
RA   Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA   Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA   She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA   Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA   Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T.,
RA   Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M.,
RA   Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K.,
RA   Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C.,
RA   Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M.,
RA   Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A.,
RA   Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M.,
RA   Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S.,
RA   Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT   "The DNA sequence and comparative analysis of human chromosome 5.";
RL   Nature 431:268-274(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain, and Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   DOMAIN.
RX   PubMed=18704115; DOI=10.1038/embor.2008.163;
RA   Geng J., Klionsky D.J.;
RT   "The Atg8 and Atg12 ubiquitin-like conjugation systems in
RT   macroautophagy. 'Protein modifications: beyond the usual suspects'
RT   review series.";
RL   EMBO Rep. 9:859-864(2008).
CC   -!- FUNCTION: Ubiquitin-like protein required for autophagy.
CC       Conjugated to ATG3 and ATG5.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O94817-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O94817-4; Sequence=VSP_018104, VSP_018105;
CC         Note=No experimental confirmation available;
CC   -!- TISSUE SPECIFICITY: Ubiquitous.
CC   -!- DOMAIN: Shares weak sequence similarity with ubiquitin family, but
CC       contains an 'ubiquitin superfold' and the C-terminal Gly is
CC       required for isopeptide linkage.
CC   -!- SIMILARITY: Belongs to the ATG12 family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH12266.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
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DR   EMBL; AB017507; BAA36493.1; -; mRNA.
DR   EMBL; AC026449; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC011033; AAH11033.1; -; mRNA.
DR   EMBL; BC012266; AAH12266.2; ALT_INIT; mRNA.
DR   IPI; IPI00005881; -.
DR   IPI; IPI00449147; -.
DR   RefSeq; NP_004698.3; NM_004707.3.
DR   UniGene; Hs.264482; -.
DR   ProteinModelPortal; O94817; -.
DR   SMR; O94817; 53-139.
DR   DIP; DIP-46466N; -.
DR   IntAct; O94817; 15.
DR   MINT; MINT-1434758; -.
DR   STRING; O94817; -.
DR   PRIDE; O94817; -.
DR   Ensembl; ENST00000274459; ENSP00000274459; ENSG00000145782.
DR   GeneID; 9140; -.
DR   KEGG; hsa:9140; -.
DR   UCSC; uc003krh.1; human.
DR   UCSC; uc003kri.1; human.
DR   CTD; 9140; -.
DR   GeneCards; GC05M115195; -.
DR   HGNC; HGNC:588; ATG12.
DR   HPA; CAB013272; -.
DR   MIM; 609608; gene.
DR   neXtProt; NX_O94817; -.
DR   PharmGKB; PA24879; -.
DR   eggNOG; prNOG17832; -.
DR   GeneTree; ENSGT00390000016654; -.
DR   HOVERGEN; HBG080875; -.
DR   InParanoid; O94817; -.
DR   PhylomeDB; O94817; -.
DR   Reactome; REACT_6900; Immune System.
DR   NextBio; 34279; -.
DR   ArrayExpress; O94817; -.
DR   Bgee; O94817; -.
DR   CleanEx; HS_ATG12; -.
DR   Genevestigator; O94817; -.
DR   GermOnline; ENSG00000145782; Homo sapiens.
DR   GO; GO:0034045; C:pre-autophagosomal structure membrane; ISS:UniProtKB.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0000045; P:autophagic vacuole assembly; ISS:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; TAS:Reactome.
DR   GO; GO:0032480; P:negative regulation of type I interferon production; TAS:Reactome.
DR   InterPro; IPR007242; Autophagy-rel_prot_12.
DR   KO; K08336; -.
DR   PANTHER; PTHR13385; APG12; 1.
DR   Pfam; PF04110; APG12; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Autophagy; Complete proteome; Cytoplasm;
KW   Isopeptide bond; Reference proteome; Ubl conjugation pathway.
FT   CHAIN         1    140       Ubiquitin-like protein ATG12.
FT                                /FTId=PRO_0000212471.
FT   CROSSLNK    140    140       Glycyl lysine isopeptide (Gly-Lys)
FT                                (interchain with K-? in acceptor
FT                                protein).
FT   VAR_SEQ      56     74       DILLKAVGDTPIMKTKKWA -> YLCESVLCSFPRPRSWNS
FT                                L (in isoform 2).
FT                                /FTId=VSP_018104.
FT   VAR_SEQ      75    140       Missing (in isoform 2).
FT                                /FTId=VSP_018105.
SQ   SEQUENCE   140 AA;  15113 MW;  109258521C2D194F CRC64;
     MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIDILLK
     AVGDTPIMKT KKWAVERTRT IQGLIDFIKK FLKLVASEQL FIYVNQSFAP SPDQEVGTLY
     ECFGSDGKLV LHYCKSQAWG
//