Ubiquitin-like protein ATG12 - Homo sapiens (Human)

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

Molecule processing

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

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O94817 (ATG12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Ubiquitin-like protein ATG12

Alternative name(s):
Autophagy-related protein 12
Short name=APG12-like

Gene names

Name:	ATG12
Synonyms:	APG12, APG12L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Ubiquitous. Ref.1
Domain	Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. Ref.4
Sequence similarities	Belongs to the ATG12 family.
Sequence caution	The sequence AAH12266.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Keywords
Biological process	Autophagy Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
PTM	Isopeptide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from sequence or structural similarity. Source: UniProtKB innate immune response Traceable author statement. Source: Reactome negative regulation of type I interferon production Traceable author statement. Source: Reactome
Cellular_component	pre-autophagosomal structure membrane Inferred from sequence or structural similarity. Source: UniProtKB protein complex Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 140

140

Ubiquitin-like protein ATG12

PRO_0000212471

Cross-link

140

Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein)

Alternative sequence

56 – 74

DILLK…TKKWA → YLCESVLCSFPRPRSWNSL in isoform 2.

VSP_018104

Alternative sequence

75 – 140

Missing in isoform 2.

VSP_018105

140

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 109258521C2D194F Show »	FASTA	140	15,113
10 20 30 40 50 60 MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIDILLK 70 80 90 100 110 120 AVGDTPIMKT KKWAVERTRT IQGLIDFIKK FLKLVASEQL FIYVNQSFAP SPDQEVGTLY 130 140 ECFGSDGKLV LHYCKSQAWG « Hide
Isoform 2 [UniParc]. Checksum: 21346AAD91E00D16 Show »	FASTA	74	7,746
10 20 30 40 50 60 MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIYLCES 70 VLCSFPRPRS WNSL « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." Mizushima N., Sugita H., Yoshimori T., Ohsumi Y. J. Biol. Chem. 273:33889-33892(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CONJUGATION TO ATG5, TISSUE SPECIFICITY.
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Ovary.
[4]	"The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. 'Protein modifications: beyond the usual suspects' review series." Geng J., Klionsky D.J. EMBO Rep. 9:859-864(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

AB017507 mRNA. Translation: BAA36493.1.
AC026449 Genomic DNA. No translation available.
BC011033 mRNA. Translation: AAH11033.1.
BC012266 mRNA. Translation: AAH12266.2. Different initiation.

IPI

IPI00005881.
IPI00449147.

RefSeq

NP_004698.3. NM_004707.3.

UniGene

Hs.264482.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GDK	X-ray	2.70	A/D	52-140	[»]
4GDL	X-ray	2.88	A	52-140	[»]

ProteinModelPortal

O94817.

ModBase

Search...

DIP

DIP-46466N.

IntAct

O94817. 16 interactions.

MINT

MINT-1434758.

STRING

9606.ENSP00000274459.

PhosphoSite

O94817.

PaxDb

O94817.

PRIDE

O94817.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENST00000500945; ENSP00000425164; ENSG00000145782.
ENST00000509910; ENSP00000425107; ENSG00000145782.

GeneID

9140.

KEGG

hsa:9140.

UCSC

uc003krh.3. human.

CTD

9140.

GeneCards

GC05M115195.

HGNC

HGNC:588. ATG12.

HPA

CAB013272.

MIM

609608. gene.

neXtProt

NX_O94817.

PharmGKB

PA24879.

GenAtlas

Search...

eggNOG

NOG236266.

HOVERGEN

HBG080875.

InParanoid

O94817.

K08336.

Reactome

REACT_6900. Immune System.

ArrayExpress

O94817.

Bgee

O94817.

CleanEx

HS_ATG12.

Genevestigator

O94817.

GermOnline

ENSG00000145782. Homo sapiens.

InterPro

IPR007242. Atg12.
[Graphical view]

PANTHER

PTHR13385. PTHR13385. 1 hit.

Pfam

PF04110. APG12. 1 hit.
[Graphical view]

ProtoNet

Search...

ChiTaRS

ATG12. human.

GenomeRNAi

9140.

NextBio

34279.

SOURCE

Search...

Entry name

ATG12_HUMAN

Accession

Primary (citable) accession number: O94817
Secondary accession number(s): Q6PJV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94817-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94817-4)

The sequence of this isoform differs from the canonical sequence as follows:
56-74: DILLKAVGDTPIMKTKKWA → YLCESVLCSFPRPRSWNSL
75-140: Missing.

Note: No experimental confirmation available.