O94817 (ATG12_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 105.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin-like protein ATG12 Alternative name(s): Autophagy-related protein 12 Short name=APG12-like | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 140 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. |
| Subcellular location | Cytoplasm By similarity. |
| Tissue specificity | Ubiquitous. Ref.1 |
| Domain | Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. Ref.4 |
| Sequence similarities | Belongs to the ATG12 family. |
| Sequence caution | The sequence AAH12266.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Autophagy Ubl conjugation pathway |
| Cellular component | Cytoplasm |
| Coding sequence diversity | Alternative splicing |
| PTM | Isopeptide bond |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | autophagic vacuole assembly Inferred from sequence or structural similarity. Source: UniProtKB innate immune responseTraceable author statement. Source: Reactome negative regulation of type I interferon productionTraceable author statement. Source: Reactome |
| Cellular_component | pre-autophagosomal structure membrane Inferred from sequence or structural similarity. Source: UniProtKB protein complexInferred from electronic annotation. Source: Compara |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O94817-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O94817-4) The sequence of this isoform differs from the canonical sequence as follows: 56-74: DILLKAVGDTPIMKTKKWA → YLCESVLCSFPRPRSWNSL 75-140: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 140 | 140 | Ubiquitin-like protein ATG12 | PRO_0000212471 | ||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||
| Cross-link | 140 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein) | ||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||
| Alternative sequence | 56 – 74 | 19 | DILLK…TKKWA → YLCESVLCSFPRPRSWNSL in isoform 2. | VSP_018104 | ||||||||||||||||||||||
| Alternative sequence | 75 – 140 | 66 | Missing in isoform 2. | VSP_018105 | ||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||
| Beta strand | 54 – 61 | 8 | ||||||||||||||||||||||||
| Beta strand | 63 – 65 | 3 | ||||||||||||||||||||||||
| Beta strand | 72 – 76 | 5 | ||||||||||||||||||||||||
| Helix | 81 – 91 | 11 | ||||||||||||||||||||||||
| Beta strand | 96 – 98 | 3 | ||||||||||||||||||||||||
| Beta strand | 101 – 104 | 4 | ||||||||||||||||||||||||
| Turn | 105 – 107 | 3 | ||||||||||||||||||||||||
| Helix | 116 – 123 | 8 | ||||||||||||||||||||||||
| Beta strand | 128 – 136 | 9 | ||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." Mizushima N., Sugita H., Yoshimori T., Ohsumi Y. J. Biol. Chem. 273:33889-33892(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CONJUGATION TO ATG5, TISSUE SPECIFICITY. |
| [2] | "The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. Rubin E.M.Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Ovary. |
| [4] | "The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. 'Protein modifications: beyond the usual suspects' review series." Geng J., Klionsky D.J. EMBO Rep. 9:859-864(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN. |
| [5] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AB017507 mRNA. Translation: BAA36493.1. AC026449 Genomic DNA. No translation available. BC011033 mRNA. Translation: AAH11033.1. BC012266 mRNA. Translation: AAH12266.2. Different initiation. | ||||||||||||||||||
| IPI | IPI00005881. IPI00449147. | ||||||||||||||||||
| RefSeq | NP_004698.3. NM_004707.3. | ||||||||||||||||||
| UniGene | Hs.264482. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | O94817. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP-46466N. | ||||||||||||||||||
| IntAct | O94817. 16 interactions. | ||||||||||||||||||
| MINT | MINT-1434758. | ||||||||||||||||||
| STRING | 9606.ENSP00000274459. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | O94817. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PaxDb | O94817. | ||||||||||||||||||
| PRIDE | O94817. | ||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENST00000500945; ENSP00000425164; ENSG00000145782. ENST00000509910; ENSP00000425107; ENSG00000145782. | ||||||||||||||||||
| GeneID | 9140. | ||||||||||||||||||
| KEGG | hsa:9140. | ||||||||||||||||||
| UCSC | uc003krh.3. human. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CTD | 9140. | ||||||||||||||||||
| GeneCards | GC05M115195. | ||||||||||||||||||
| HGNC | HGNC:588. ATG12. | ||||||||||||||||||
| HPA | CAB013272. | ||||||||||||||||||
| MIM | 609608. gene. | ||||||||||||||||||
| neXtProt | NX_O94817. | ||||||||||||||||||
| PharmGKB | PA24879. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| eggNOG | NOG236266. | ||||||||||||||||||
| HOVERGEN | HBG080875. | ||||||||||||||||||
| InParanoid | O94817. | ||||||||||||||||||
| KO | K08336. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| Reactome | REACT_6900. Immune System. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | O94817. | ||||||||||||||||||
| Bgee | O94817. | ||||||||||||||||||
| CleanEx | HS_ATG12. | ||||||||||||||||||
| Genevestigator | O94817. | ||||||||||||||||||
| GermOnline | ENSG00000145782. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR007242. Atg12. [Graphical view] | ||||||||||||||||||
| PANTHER | PTHR13385. PTHR13385. 1 hit. | ||||||||||||||||||
| Pfam | PF04110. APG12. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other | |||||||||||||||||||
| ChiTaRS | ATG12. human. | ||||||||||||||||||
| GenomeRNAi | 9140. | ||||||||||||||||||
| NextBio | 34279. | ||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | ATG12_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O94817 Secondary accession number(s): Q6PJV2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
