Reviewed,
UniProtKB/Swiss-Prot O94817 (ATG12_HUMAN)
Last modified
June 16, 2009.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Autophagy-related protein 12 Alternative name(s): APG12-like | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 140 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Required for autophagy. |
| Subunit structure | Conjugated to ATG5. |
| Subcellular location | Cytoplasm By similarity. |
| Tissue specificity | Ubiquitous. Ref.1 |
| Sequence similarities | Belongs to the ATG12 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Autophagy Ubl conjugation pathway |
| Cellular component | Cytoplasm |
| Coding sequence diversity | Alternative splicing |
| PTM | Isopeptide bond |
| Gene Ontology (GO) | |
| Biological process | autophagic vacuole formation Inferred from sequence or structural similarity. Source: UniProtKB modification-dependent protein catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | pre-autophagosomal structure membrane Inferred from sequence or structural similarity. Source: UniProtKB |
| Molecular function | protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ATG10 | Q9H0Y0 | 1 | EBI-746742,EBI-1048913 | |
| ATG16L1 | Q676U5 | 1 | EBI-746742,EBI-535909 | |
| ATG5 | Q9H1Y0 | 1 | EBI-746742,EBI-1047414 | |
| AUP1 | Q9Y679 | 1 | EBI-746742,EBI-1058701 | |
| DHX36 | Q9H2U1 | 1 | EBI-746742,EBI-1047643 | |
| OTUD4 | Q01804 | 1 | EBI-746742,EBI-1054396 | |
| SF3A1 | Q15459 | 1 | EBI-746742,EBI-1054743 | |
| SF3B1 | O75533 | 1 | EBI-746742,EBI-876542 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O94817-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O94817-4) The sequence of this isoform differs from the canonical sequence as follows: 56-74: DILLKAVGDTPIMKTKKWA → YLCESVLCSFPRPRSWNSL 75-140: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 140 | 140 | Autophagy-related protein 12 | PRO_0000212471 | |||||
Amino acid modifications | |||||||||
| Cross-link | 140 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-130 in ATG5) | |||||||
Natural variations | |||||||||
| Alternative sequence | 56 – 74 | 19 | DILLK…TKKWA → YLCESVLCSFPRPRSWNSL in isoform 2. | VSP_018104 | |||||
| Alternative sequence | 75 – 140 | 66 | Missing in isoform 2. | VSP_018105 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." Mizushima N., Sugita H., Yoshimori T., Ohsumi Y. J. Biol. Chem. 273:33889-33892(1998) [PubMed: 9852036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CONJUGATION TO ATG5, TISSUE SPECIFICITY. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Ovary. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB017507 mRNA. Translation: BAA36493.1. BC011033 mRNA. Translation: AAH11033.1. BC012266 mRNA. Translation: AAH12266.2. Different initiation. | |
| IPI | IPI00005881. IPI00449147. |
| RefSeq | NP_004698.2. |
| UniGene | Hs.264482 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O94817. 47 interactions. |
Genome annotation databases | |
| Ensembl | ENSG00000145782. Homo sapiens. [Contig view] |
| GeneID | 9140. |
| KEGG | hsa:9140. |
Organism-specific databases | |
| GeneCards | GC05M115195. |
| H-InvDB | HIX0005105. |
| HGNC | HGNC:588. ATG12. |
| HPA | CAB013272. |
| MIM | 609608. gene. |
| PharmGKB | PA24879. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | O94817. |
| HOVERGEN | O94817. |
Gene expression databases | |
| ArrayExpress | O94817. |
| Bgee | O94817. |
| CleanEx | HS_ATG12. |
| GermOnline | ENSG00000145782. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR007242. Autophagy-rel_prot_12. [Graphical view] |
| PANTHER | PTHR13385. APG12. 1 hit. |
| Pfam | PF04110. APG12. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 34279. |
| SOURCE | Search... |
Entry information
| Entry name | ATG12_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O94817 Secondary accession number(s): Q6PJV2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


