Reviewed, UniProtKB/Swiss-Prot O94811 (TPPP_HUMAN)

Names and origin

Protein names

Recommended name:
    Tubulin polymerization-promoting protein
      Short name=TPPP
Alternative name(s):
    25 kDa brain-specific protein
    p25-alpha
    p24
    p25

Gene names

Name:	TPPP
Synonyms:	TPPP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.
Subunit structure	May form dimers. Binds tubulin; binding is inhibited by GTP. Interacts with GSK3 By similarity.
Tissue specificity	Widely expressed.
Post-translational modification	Phosphorylated by TPK II. Poor substrate for GSK3 By similarity.
Sequence similarities	Belongs to the TPPP family.

Ontologies

Keywords
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	microtubule bundle formation Inferred from sequence or structural similarity. Source: HGNC microtubule polymerization Inferred from sequence or structural similarity. Source: HGNC positive regulation of protein polymerization Inferred from direct assay. Source: HGNC
Cellular component	perinuclear region of cytoplasm Inferred from direct assay. Source: HGNC soluble fraction Inferred from sequence or structural similarity. Source: HGNC
Molecular function	microtubule binding Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Tubulin polymerization-promoting protein		PRO_0000221135
Amino acid modifications
	Modified residue	14	1	Phosphothreonine
	Modified residue	18	1	Phosphoserine
	Modified residue	32	1	Phosphoserine
	Glycosylation	152	1	O-linked (GlcNAc) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94811-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: F8B2C814EA59129A	FASTA	219	23,694
	10 20 30 40 50 60 MADKAKPAKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV 70 80 90 100 110 120 HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL 130 140 150 160 170 180 EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF 190 200 210 DPSGKGKGKA GRVDLVDESG YVSGYKHAGT YDQKVQGGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human gene whose product shares significant homology with the bovine brain-specific protein p25 on chromosome 5p15.3." Seki N., Hattori A., Sugano S., Suzuki Y., Nakagawara A., Muramatsu M.-A., Hori T.-A., Saito T. J. Hum. Genet. 44:121-122(1999) [PubMed: 10083737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Neuroblastoma.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Phosphoproteomic analysis of synaptosomes from human cerebral cortex." DeGiorgis J.A., Jaffe H., Moreira J.E., Carlotti C.G. Jr., Leite J.P., Pant H.C., Dosemeci A. J. Proteome Res. 4:306-315(2005) [PubMed: 15822905] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-14 AND SER-18, MASS SPECTROMETRY. Tissue: Brain cortex.
[5]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB017016 mRNA. Translation: BAA36164.1. BT020035 mRNA. Translation: AAV38838.1. BC040496 mRNA. Translation: AAH40496.1. Different initiation.
RefSeq	NP_008961.1.
UniGene	Hs.481466
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	O94811.
Genome annotation databases
Ensembl	ENSG00000171368. Homo sapiens. [Contig view]
GeneID	11076.
KEGG	hsa:11076.
Organism-specific databases
H-InvDB	HIX0004710.
HGNC	HGNC:24164. TPPP.
MIM	608773. gene.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O94811.
HOVERGEN	O94811.
Gene expression databases
ArrayExpress	O94811.
CleanEx	HS_TPPP.
Family and domain databases
InterPro	IPR008907. P25-alpha. [Graphical view]
PANTHER	PTHR12932. P25-alpha. 1 hit.
Pfam	PF05517. p25-alpha. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	O94811.
NextBio	42106.
SOURCE	Search...

Entry information

Entry name

TPPP_HUMAN

Accession

Primary (citable) accession number: O94811

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)