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O94808

- GFPT2_HUMAN

UniProt

O94808 - GFPT2_HUMAN

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Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
Gene
GFPT2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: ProtInc

GO - Biological processi

  1. UDP-N-acetylglucosamine biosynthetic process Source: Reactome
  2. carbohydrate biosynthetic process Source: InterPro
  3. cellular protein metabolic process Source: Reactome
  4. dolichol-linked oligosaccharide biosynthetic process Source: Reactome
  5. energy reserve metabolic process Source: ProtInc
  6. fructose 6-phosphate metabolic process Source: ProtInc
  7. glutamine metabolic process Source: UniProtKB-KW
  8. post-translational protein modification Source: Reactome
  9. protein N-linked glycosylation via asparagine Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_22394. Synthesis of UDP-N-acetyl-glucosamine.
UniPathwayiUPA00113; UER00528.

Protein family/group databases

MEROPSiC44.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 2
Glutamine:fructose-6-phosphate amidotransferase 2
Short name:
GFAT 2
Short name:
GFAT2
Hexosephosphate aminotransferase 2
Gene namesi
Name:GFPT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:4242. GFPT2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28652.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 682681Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
PRO_0000135283Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei245 – 2451Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94808.
PaxDbiO94808.
PRIDEiO94808.

PTM databases

PhosphoSiteiO94808.

Expressioni

Tissue specificityi

Highest levels of expression in heart, placenta, and spinal cord.

Gene expression databases

ArrayExpressiO94808.
BgeeiO94808.
CleanExiHS_GFPT2.
GenevestigatoriO94808.

Organism-specific databases

HPAiHPA049528.
HPA056892.

Interactioni

Protein-protein interaction databases

BioGridi115270. 5 interactions.
STRINGi9606.ENSP00000253778.

Structurei

3D structure databases

ProteinModelPortaliO94808.
SMRiO94808. Positions 16-682.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 288287Glutamine amidotransferase type-2
Add
BLAST
Domaini360 – 499140SIS 1
Add
BLAST
Domaini531 – 672142SIS 2
Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiO94808.
KOiK00820.
OMAiRCLPEMI.
OrthoDBiEOG73NG2S.
PhylomeDBiO94808.
TreeFamiTF300864.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O94808-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK    50
ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS 100
AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA 150
KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR 200
GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA 250
VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS 300
DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN 350
TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE 400
LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT 450
VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL 500
QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY 550
ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF 600
AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS 650
VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE 682
Length:682
Mass (Da):76,931
Last modified:January 23, 2007 - v3
Checksum:iA090D75526F3192B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti471 – 4711I → V.1 Publication
Corresponds to variant rs2303007 [ dbSNP | Ensembl ].
VAR_013311

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB016789 mRNA. Translation: BAA74731.1.
BT009818 mRNA. Translation: AAP88820.1.
BC000012 mRNA. Translation: AAH00012.1.
CCDSiCCDS43411.1.
RefSeqiNP_005101.1. NM_005110.2.
UniGeneiHs.696497.

Genome annotation databases

EnsembliENST00000253778; ENSP00000253778; ENSG00000131459.
GeneIDi9945.
KEGGihsa:9945.
UCSCiuc003mlw.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB016789 mRNA. Translation: BAA74731.1 .
BT009818 mRNA. Translation: AAP88820.1 .
BC000012 mRNA. Translation: AAH00012.1 .
CCDSi CCDS43411.1.
RefSeqi NP_005101.1. NM_005110.2.
UniGenei Hs.696497.

3D structure databases

ProteinModelPortali O94808.
SMRi O94808. Positions 16-682.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115270. 5 interactions.
STRINGi 9606.ENSP00000253778.

Chemistry

DrugBanki DB00130. L-Glutamine.

Protein family/group databases

MEROPSi C44.972.

PTM databases

PhosphoSitei O94808.

Proteomic databases

MaxQBi O94808.
PaxDbi O94808.
PRIDEi O94808.

Protocols and materials databases

DNASUi 9945.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000253778 ; ENSP00000253778 ; ENSG00000131459 .
GeneIDi 9945.
KEGGi hsa:9945.
UCSCi uc003mlw.1. human.

Organism-specific databases

CTDi 9945.
GeneCardsi GC05M179727.
HGNCi HGNC:4242. GFPT2.
HPAi HPA049528.
HPA056892.
MIMi 603865. gene.
neXtProti NX_O94808.
PharmGKBi PA28652.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0449.
HOGENOMi HOG000258898.
HOVERGENi HBG051724.
InParanoidi O94808.
KOi K00820.
OMAi RCLPEMI.
OrthoDBi EOG73NG2S.
PhylomeDBi O94808.
TreeFami TF300864.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00528 .
Reactomei REACT_22394. Synthesis of UDP-N-acetyl-glucosamine.

Miscellaneous databases

GenomeRNAii 9945.
NextBioi 37520.
PROi O94808.
SOURCEi Search...

Gene expression databases

ArrayExpressi O94808.
Bgeei O94808.
CleanExi HS_GFPT2.
Genevestigatori O94808.

Family and domain databases

Gene3Di 3.60.20.10. 2 hits.
InterProi IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
Pfami PF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 2 hits.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and mapping of a novel subtype of glutamine:fructose-6-phosphate amidotransferase (GFAT2) in human and mouse."
    Oki T., Yamazaki K., Kuromitsu J., Okada M., Tanaka I.
    Genomics 57:227-234(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Thymus.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-471.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGFPT2_HUMAN
AccessioniPrimary (citable) accession number: O94808
Secondary accession number(s): Q53XM2, Q9BWS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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