Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase D3

Gene

PRKD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei605ATPPROSITE-ProRule annotation1
Active sitei699Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri154 – 204Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri271 – 321Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi582 – 590ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW
  • protein kinase C activity Source: ProtInc
  • protein serine/threonine kinase activity Source: Reactome

GO - Biological processi

  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: ProtInc
  • protein kinase D signaling Source: GO_Central
  • protein phosphorylation Source: ProtInc
  • sphingolipid biosynthetic process Source: Reactome

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13 2681
ReactomeiR-HSA-1660661 Sphingolipid de novo biosynthesis
SignaLinkiO94806
SIGNORiO94806

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase D3 (EC:2.7.11.13)
Alternative name(s):
Protein kinase C nu type
Protein kinase EPK2
nPKC-nu
Gene namesi
Name:PRKD3
Synonyms:EPK2, PRKCN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115825.9
HGNCiHGNC:9408 PRKD3
MIMi607077 gene
neXtProtiNX_O94806

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi156T → A: Slight loss in ability to bind DAG and phorbol-ester; when associated with F-158. 1 Publication1
Mutagenesisi158Y → F: Slight loss in ability to bind DAG and phorbol-ester; when associated with A-156. 1 Publication1
Mutagenesisi165P → G: No effect on ability to bind phorbol ester, loss of ability to bind DAG, reduced DAG-induced membrane translocation. 1 Publication1
Mutagenesisi166T → A: Slight loss in ability to bind DAG and phorbol-ester. 1 Publication1
Mutagenesisi170Y → F: Slight loss in ability to bind DAG and phorbol-ester. 1 Publication1
Mutagenesisi282P → G: No effect on ability to bind phorbol ester, increase in ability to bind DAG. 1 Publication1
Mutagenesisi284I → V: Slight increase in ability to bind DAG, no effect on phorbol-ester binding. 1
Mutagenesisi293K → W: Increased ability to bind DAG, no effect on phorbol-ester binding. 1 Publication1

Organism-specific databases

DisGeNETi23683
OpenTargetsiENSG00000115825
PharmGKBiPA33772

Chemistry databases

ChEMBLiCHEMBL2595
GuidetoPHARMACOLOGYi2174

Polymorphism and mutation databases

BioMutaiPRKD3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557171 – 890Serine/threonine-protein kinase D3Add BLAST890

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineCombined sources1
Modified residuei27PhosphoserineCombined sources1
Modified residuei37PhosphoserineCombined sources1
Modified residuei41PhosphoserineCombined sources1
Modified residuei44PhosphoserineCombined sources1
Modified residuei213PhosphoserineCombined sources1
Modified residuei216PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei391PhosphoserineBy similarity1
Modified residuei395PhosphoserineBy similarity1
Modified residuei426PhosphotyrosineBy similarity1
Modified residuei442PhosphoserineBy similarity1
Modified residuei457PhosphotyrosineBy similarity1
Modified residuei535PhosphothreonineCombined sources1
Modified residuei539PhosphoserineBy similarity1
Modified residuei731Phosphoserine; by PKCBy similarity1
Modified residuei735Phosphoserine; by autocatalysisBy similarity1
Modified residuei742PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO94806
MaxQBiO94806
PaxDbiO94806
PeptideAtlasiO94806
PRIDEiO94806

PTM databases

iPTMnetiO94806
PhosphoSitePlusiO94806

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000115825
CleanExiHS_PRKD3
ExpressionAtlasiO94806 baseline and differential
GenevisibleiO94806 HS

Organism-specific databases

HPAiHPA029529

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
VAMP2P630277EBI-1255366,EBI-520113

Protein-protein interaction databases

BioGridi117199, 11 interactors
IntActiO94806, 13 interactors
MINTiO94806
STRINGi9606.ENSP00000234179

Chemistry databases

BindingDBiO94806

Structurei

Secondary structure

1890
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi417 – 429Combined sources13
Beta strandi434 – 443Combined sources10
Beta strandi445 – 449Combined sources5
Turni462 – 464Combined sources3
Beta strandi477 – 479Combined sources3
Beta strandi485 – 489Combined sources5
Beta strandi494 – 497Combined sources4
Turni508 – 514Combined sources7
Helixi517 – 532Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D9ZNMR-A417-532[»]
ProteinModelPortaliO94806
SMRiO94806
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO94806

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini416 – 532PHPROSITE-ProRule annotationAdd BLAST117
Domaini576 – 832Protein kinasePROSITE-ProRule annotationAdd BLAST257

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri154 – 204Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri271 – 321Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4236 Eukaryota
ENOG410XQZ3 LUCA
GeneTreeiENSGT00910000144054
HOGENOMiHOG000015135
HOVERGENiHBG003564
InParanoidiO94806
KOiK06070
OMAiGRPTICQ
OrthoDBiEOG091G02RG
PhylomeDBiO94806
TreeFamiTF314320

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR015727 Protein_Kinase_C_mu-related
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR22968 PTHR22968, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PIRSFiPIRSF000552 PKC_mu_nu_D2, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00233 PH, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O94806-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSANNSPPSA QKSVLPTAIP AVLPAASPCS SPKTGLSARL SNGSFSAPSL
60 70 80 90 100
TNSRGSVHTV SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC
110 120 130 140 150
GFFGMYDKIL LFRHDMNSEN ILQLITSADE IHEGDLVEVV LSALATVEDF
160 170 180 190 200
QIRPHTLYVH SYKAPTFCDY CGEMLWGLVR QGLKCEGCGL NYHKRCAFKI
210 220 230 240 250
PNNCSGVRKR RLSNVSLPGP GLSVPRPLQP EYVALPSEES HVHQEPSKRI
260 270 280 290 300
PSWSGRPIWM EKMVMCRVKV PHTFAVHSYT RPTICQYCKR LLKGLFRQGM
310 320 330 340 350
QCKDCKFNCH KRCASKVPRD CLGEVTFNGE PSSLGTDTDI PMDIDNNDIN
360 370 380 390 400
SDSSRGLDDT EEPSPPEDKM FFLDPSDLDV ERDEEAVKTI SPSTSNNIPL
410 420 430 440 450
MRVVQSIKHT KRKSSTMVKE GWMVHYTSRD NLRKRHYWRL DSKCLTLFQN
460 470 480 490 500
ESGSKYYKEI PLSEILRISS PRDFTNISQG SNPHCFEIIT DTMVYFVGEN
510 520 530 540 550
NGDSSHNPVL AATGVGLDVA QSWEKAIRQA LMPVTPQASV CTSPGQGKDH
560 570 580 590 600
KDLSTSISVS NCQIQENVDI STVYQIFADE VLGSGQFGIV YGGKHRKTGR
610 620 630 640 650
DVAIKVIDKM RFPTKQESQL RNEVAILQNL HHPGIVNLEC MFETPERVFV
660 670 680 690 700
VMEKLHGDML EMILSSEKSR LPERITKFMV TQILVALRNL HFKNIVHCDL
710 720 730 740 750
KPENVLLASA EPFPQVKLCD FGFARIIGEK SFRRSVVGTP AYLAPEVLRS
760 770 780 790 800
KGYNRSLDMW SVGVIIYVSL SGTFPFNEDE DINDQIQNAA FMYPPNPWRE
810 820 830 840 850
ISGEAIDLIN NLLQVKMRKR YSVDKSLSHP WLQDYQTWLD LREFETRIGE
860 870 880 890
RYITHESDDA RWEIHAYTHN LVYPKHFIMA PNPDDMEEDP
Length:890
Mass (Da):100,471
Last modified:May 1, 1999 - v1
Checksum:i66D5E7E7235064F5
GO
Isoform 2 (identifier: O94806-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     595-611: HRKTGRDVAIKVIDKMR → QLQPFAYCTHYFKNWKM
     612-890: Missing.

Show »
Length:611
Mass (Da):68,123
Checksum:i1714FAD7DA82C942
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03714742N → D. Corresponds to variant dbSNP:rs11896614Ensembl.1
Natural variantiVAR_037148128A → T1 PublicationCorresponds to variant dbSNP:rs17852819Ensembl.1
Natural variantiVAR_050561225P → S. Corresponds to variant dbSNP:rs34280934Ensembl.1
Natural variantiVAR_061532445L → I. Corresponds to variant dbSNP:rs55912911Ensembl.1
Natural variantiVAR_037149546Q → R1 PublicationCorresponds to variant dbSNP:rs17856887Ensembl.1
Natural variantiVAR_042336716V → M in a glioblastoma multiforme sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029405595 – 611HRKTG…IDKMR → QLQPFAYCTHYFKNWKM in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_029406612 – 890Missing in isoform 2. 1 PublicationAdd BLAST279

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015982 mRNA Translation: BAA36514.1
AC007390 Genomic DNA Translation: AAY14817.1
CH471053 Genomic DNA Translation: EAX00398.1
CH471053 Genomic DNA Translation: EAX00399.1
BC030706 mRNA Translation: AAH30706.1
CCDSiCCDS1789.1 [O94806-1]
RefSeqiNP_005804.1, NM_005813.4 [O94806-1]
XP_005264294.1, XM_005264237.3 [O94806-1]
UniGeneiHs.660757
Hs.716034

Genome annotation databases

EnsembliENST00000234179; ENSP00000234179; ENSG00000115825 [O94806-1]
ENST00000379066; ENSP00000368356; ENSG00000115825 [O94806-1]
GeneIDi23683
KEGGihsa:23683
UCSCiuc002rqd.4 human [O94806-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKPCD3_HUMAN
AccessioniPrimary (citable) accession number: O94806
Secondary accession number(s): D6W587, Q53TR7, Q8NEL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health