Reviewed,
UniProtKB/Swiss-Prot O94806 (KPCD3_HUMAN)
Last modified
November 25, 2008.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (7) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase D3 EC=2.7.11.13 Alternative name(s): Protein kinase C nu type nPKC-nu Protein kinase EPK2 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 890 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. Upon diacylglycerol-activation, phosphorylates a range of cellular proteins. Also serves as the receptor for phorbol esters, a class of tumor promoters. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Tissue specificity | Ubiquitous. |
| Sequence similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. |
Ontologies
Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Phorbol-ester binding Repeat Zinc-finger |
| Ligand | ATP-binding Metal-binding Nucleotide-binding Zinc |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | 3D-structure |
Gene Ontology (GO) | |
| Biological process | activation of protein kinase C activity Ref.1 Traceable author statement. Source: ProtInc protein amino acid phosphorylation Ref.1Traceable author statement. Source: ProtInc |
| Molecular function | ATP binding Inferred from electronic annotation. Source: InterPro diacylglycerol bindingInferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct protein kinase C activity Ref.1Traceable author statement. Source: ProtInc zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O94806-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O94806-2) The sequence of this isoform differs from the canonical sequence as follows: 595-611: HRKTGRDVAIKVIDKMR → QLQPFAYCTHYFKNWKM 612-890: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 890 | 890 | Serine/threonine-protein kinase D3 | PRO_0000055717 | |||||
Regions | |||||||||
| Domain | 416 – 532 | 117 | PH | ||||||
| Domain | 576 – 832 | 257 | Protein kinase | ||||||
| Zinc finger | 154 – 204 | 51 | Phorbol-ester/DAG-type 1 | ||||||
| Zinc finger | 271 – 321 | 51 | Phorbol-ester/DAG-type 2 | ||||||
| Nucleotide binding | 582 – 590 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 699 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 605 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 41 | 1 | Phosphoserine | ||||||
| Modified residue | 44 | 1 | Phosphoserine | ||||||
| Modified residue | 213 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 216 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 364 | 1 | Phosphoserine | ||||||
| Modified residue | 391 | 1 | Phosphoserine | ||||||
| Modified residue | 735 | 1 | Phosphoserine | ||||||
Natural variations | |||||||||
| Alternative sequence | 595 – 611 | 17 | HRKTG…IDKMR → QLQPFAYCTHYFKNWKM in isoform 2. | VSP_029405 | |||||
| Alternative sequence | 612 – 890 | 279 | Missing in isoform 2. | VSP_029406 | |||||
| Natural variant | 42 | 1 | N → D: dbSNP rs11896614. | VAR_037147 | |||||
| Natural variant | 128 | 1 | A → T: dbSNP rs17852819. | VAR_037148 | |||||
| Natural variant | 546 | 1 | Q → R: dbSNP rs17856887. | VAR_037149 | |||||
| Natural variant | 716 | 1 | V → M in a glioblastoma multiforme sample; somatic mutation. | VAR_042336 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "PKCnu, a new member of the protein kinase C family, composes a fourth subfamily with PKCmu." Hayashi A., Seki N., Hattori A., Kozuma S., Saito T. Biochim. Biophys. Acta 1450:99-106(1999) [PubMed: 10231560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Embryo. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS THR-128 AND ARG-546. Tissue: Testis. |
| [3] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41 AND SER-44, MASS SPECTROMETRY. Tissue: Epithelium. |
| [4] | "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, MASS SPECTROMETRY. Tissue: Epithelium. |
| [5] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391 AND SER-735, MASS SPECTROMETRY. |
| [6] | "Solution structure of the PH domain of protein kinase C, nu type from human." RIKEN structural genomics initiative (RSGI) Submitted (JAN-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 414-532. |
| [7] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-716. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AB015982 mRNA. Translation: BAA36514.1. BC030706 mRNA. Translation: AAH30706.1. | |||||||||||||
| RefSeq | NP_005804.1. | ||||||||||||
| UniGene | Hs.660757 Hs.696257 | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | O94806. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | O94806. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000115825. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 23683. | ||||||||||||
| KEGG | hsa:23683. | ||||||||||||
Organism-specific databases | |||||||||||||
| H-InvDB | HIX0001974. | ||||||||||||
| HGNC | HGNC:9408. PRKD3. | ||||||||||||
| MIM | 607077. gene. | ||||||||||||
| PharmGKB | PA33772. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | O94806. | ||||||||||||
| HOVERGEN | O94806. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | O94806. | ||||||||||||
| CleanEx | HS_PRKD3. | ||||||||||||
| GermOnline | ENSG00000115825. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR002219. DAG_PE_bd. IPR001849. PH. IPR011993. PH_type. IPR015727. PKC_mu_like. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. | ||||||||||||
| PANTHER | PTHR22968. PKC_mu_like. 1 hit. | ||||||||||||
| Pfam | PF00130. C1_1. 2 hits. PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR00008. DAGPEDOMAIN. | ||||||||||||
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| SMART | SM00109. C1. 2 hits. SM00233. PH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 46561. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | KPCD3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O94806 Secondary accession number(s): Q8NEL8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

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