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Reviewed, UniProtKB/Swiss-Prot O94806 (KPCD3_HUMAN)

Last modified November 25, 2008. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase D3
    EC=2.7.11.13
Alternative name(s):
    Protein kinase C nu type
    nPKC-nu
    Protein kinase EPK2
Gene names
Name: PRKD3
Synonyms: EPK2, PRKCN
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. Upon diacylglycerol-activation, phosphorylates a range of cellular proteins. Also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

Contains 1 PH domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

VAMP2P630272EBI-1255366,EBI-520113

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O94806-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O94806-2)

The sequence of this isoform differs from the canonical sequence as follows:
     595-611: HRKTGRDVAIKVIDKMR → QLQPFAYCTHYFKNWKM
     612-890: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Serine/threonine-protein kinase D3
PRO_0000055717

Regions

Domain416 – 532117PH
Domain576 – 832257Protein kinase
Zinc finger154 – 20451Phorbol-ester/DAG-type 1
Zinc finger271 – 32151Phorbol-ester/DAG-type 2
Nucleotide binding582 – 5909ATP By similarity

Sites

Active site6991Proton acceptor By similarity
Binding site6051ATP By similarity

Amino acid modifications

Modified residue411Phosphoserine
Modified residue441Phosphoserine
Modified residue2131Phosphoserine By similarity
Modified residue2161Phosphoserine By similarity
Modified residue3641Phosphoserine
Modified residue3911Phosphoserine
Modified residue7351Phosphoserine

Natural variations

Alternative sequence595 – 61117HRKTG…IDKMR → QLQPFAYCTHYFKNWKM in isoform 2.
VSP_029405
Alternative sequence612 – 890279Missing in isoform 2.
VSP_029406
Natural variant421N → D: dbSNP rs11896614.
VAR_037147
Natural variant1281A → T: dbSNP rs17852819.
VAR_037148
Natural variant5461Q → R: dbSNP rs17856887.
VAR_037149
Natural variant7161V → M in a glioblastoma multiforme sample; somatic mutation.
VAR_042336

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 66D5E7E7235064F5

FASTA890100,471
        10         20         30         40         50         60 
MSANNSPPSA QKSVLPTAIP AVLPAASPCS SPKTGLSARL SNGSFSAPSL TNSRGSVHTV 

        70         80         90        100        110        120 
SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC GFFGMYDKIL LFRHDMNSEN 

       130        140        150        160        170        180 
ILQLITSADE IHEGDLVEVV LSALATVEDF QIRPHTLYVH SYKAPTFCDY CGEMLWGLVR 

       190        200        210        220        230        240 
QGLKCEGCGL NYHKRCAFKI PNNCSGVRKR RLSNVSLPGP GLSVPRPLQP EYVALPSEES 

       250        260        270        280        290        300 
HVHQEPSKRI PSWSGRPIWM EKMVMCRVKV PHTFAVHSYT RPTICQYCKR LLKGLFRQGM 

       310        320        330        340        350        360 
QCKDCKFNCH KRCASKVPRD CLGEVTFNGE PSSLGTDTDI PMDIDNNDIN SDSSRGLDDT 

       370        380        390        400        410        420 
EEPSPPEDKM FFLDPSDLDV ERDEEAVKTI SPSTSNNIPL MRVVQSIKHT KRKSSTMVKE 

       430        440        450        460        470        480 
GWMVHYTSRD NLRKRHYWRL DSKCLTLFQN ESGSKYYKEI PLSEILRISS PRDFTNISQG 

       490        500        510        520        530        540 
SNPHCFEIIT DTMVYFVGEN NGDSSHNPVL AATGVGLDVA QSWEKAIRQA LMPVTPQASV 

       550        560        570        580        590        600 
CTSPGQGKDH KDLSTSISVS NCQIQENVDI STVYQIFADE VLGSGQFGIV YGGKHRKTGR 

       610        620        630        640        650        660 
DVAIKVIDKM RFPTKQESQL RNEVAILQNL HHPGIVNLEC MFETPERVFV VMEKLHGDML 

       670        680        690        700        710        720 
EMILSSEKSR LPERITKFMV TQILVALRNL HFKNIVHCDL KPENVLLASA EPFPQVKLCD 

       730        740        750        760        770        780 
FGFARIIGEK SFRRSVVGTP AYLAPEVLRS KGYNRSLDMW SVGVIIYVSL SGTFPFNEDE 

       790        800        810        820        830        840 
DINDQIQNAA FMYPPNPWRE ISGEAIDLIN NLLQVKMRKR YSVDKSLSHP WLQDYQTWLD 

       850        860        870        880        890 
LREFETRIGE RYITHESDDA RWEIHAYTHN LVYPKHFIMA PNPDDMEEDP

« Hide

Isoform 2 [UniParc].

Checksum: 1714FAD7DA82C942
Show »

61168,123

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References

« Hide 'large scale' references
[1]"PKCnu, a new member of the protein kinase C family, composes a fourth subfamily with PKCmu."
Hayashi A., Seki N., Hattori A., Kozuma S., Saito T.
Biochim. Biophys. Acta 1450:99-106(1999) [PubMed: 10231560] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS THR-128 AND ARG-546.
Tissue: Testis.
[3]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41 AND SER-44, MASS SPECTROMETRY.
Tissue: Epithelium.
[4]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, MASS SPECTROMETRY.
Tissue: Epithelium.
[5]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391 AND SER-735, MASS SPECTROMETRY.
[6]"Solution structure of the PH domain of protein kinase C, nu type from human."
RIKEN structural genomics initiative (RSGI)
Submitted (JAN-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 414-532.
[7]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed: 17344846] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-716.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AB015982 mRNA. Translation: BAA36514.1.
BC030706 mRNA. Translation: AAH30706.1.
RefSeqNP_005804.1.
UniGeneHs.660757
Hs.696257

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2D9ZNMR-A414-532[»]
ModBaseSearch...

Protein-protein interaction databases

IntActO94806.

PTM databases

PhosphoSiteO94806.

Genome annotation databases

EnsemblENSG00000115825. Homo sapiens. [Contig view]
GeneID23683.
KEGGhsa:23683.

Organism-specific databases

H-InvDBHIX0001974.
HGNCHGNC:9408. PRKD3.
MIM607077. gene.
PharmGKBPA33772.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMO94806.
HOVERGENO94806.

Gene expression databases

ArrayExpressO94806.
CleanExHS_PRKD3.
GermOnlineENSG00000115825. Homo sapiens.

Family and domain databases

InterProIPR002219. DAG_PE_bd.
IPR001849. PH.
IPR011993. PH_type.
IPR015727. PKC_mu_like.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_bd_CS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
PANTHERPTHR22968. PKC_mu_like. 1 hit.
PfamPF00130. C1_1. 2 hits.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR00008. DAGPEDOMAIN.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00109. C1. 2 hits.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio46561.
SOURCESearch...

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Entry information

Entry nameKPCD3_HUMAN
AccessionPrimary (citable) accession number: O94806
Secondary accession number(s): Q8NEL8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: November 25, 2008
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents