O94769 (ECM2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Extracellular matrix protein 2

Alternative name(s):
Matrix glycoprotein SC1/ECM2

Gene names

Name:

ECM2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Promotes matrix assembly and cell adhesiveness By similarity.
Subunit structure	Interacts with numerous extracellular matrix proteins By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Expressed predominantly in adipose tissue as well as female-specific organs such as mammary gland, ovary, and uterus.
Sequence similarities	Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 13 LRR (leucine-rich) repeats. Contains 1 LRRNT domain. Contains 1 VWFC domain.
Sequence caution	The sequence CAD97940.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Leucine-rich repeat Repeat Signal
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-matrix adhesion Traceable author statement Ref.1. Source: ProtInc extracellular matrix organization Inferred from electronic annotation. Source: Compara positive regulation of cell-substrate adhesion Inferred from electronic annotation. Source: Compara
Cellular_component	interstitial matrix Inferred from electronic annotation. Source: Compara proteinaceous extracellular matrix Traceable author statement Ref.1. Source: ProtInc
Molecular_function	heparin binding Inferred from electronic annotation. Source: Compara integrin binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94769-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94769-2)

The sequence of this isoform differs from the canonical sequence as follows:
     161-182: Missing.
     645-699: NILPEEICNA...SIVLKPQNIK → CVSDAVLETVTNRSDVAFPLW

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 699	679	Extracellular matrix protein 2		PRO_0000032731
Regions
	Domain	101 – 158	58	VWFC
	Domain	307 – 344	38	LRRNT
	Repeat	368 – 388	21	LRR 1
	Repeat	394 – 415	22	LRR 2
	Repeat	416 – 436	21	LRR 3
	Repeat	439 – 459	21	LRR 4
	Repeat	465 – 484	20	LRR 5
	Repeat	486 – 507	22	LRR 6
	Repeat	510 – 530	21	LRR 7
	Repeat	536 – 557	22	LRR 8
	Repeat	558 – 578	21	LRR 9
	Repeat	582 – 602	21	LRR 10
	Repeat	609 – 630	22	LRR 11
	Repeat	632 – 653	22	LRR 12
	Repeat	661 – 684	24	LRR 13
	Motif	294 – 296	3	Cell attachment site Potential
	Compositional bias	270 – 281	12	Poly-Glu
Amino acid modifications
	Modified residue	213	1	Phosphoserine By similarity
	Glycosylation	378	1	N-linked (GlcNAc...) Potential
	Glycosylation	449	1	N-linked (GlcNAc...) Potential
	Glycosylation	506	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	161 – 182	22	Missing in isoform 2.		VSP_039114
	Alternative sequence	645 – 699	55	NILPE…PQNIK → CVSDAVLETVTNRSDVAFPL W in isoform 2.		VSP_039115
	Natural variant	56	1	Q → P. Ref.3 Corresponds to variant rs10120210 [ dbSNP | Ensembl ].		VAR_024646
	Natural variant	109	1	T → S. Corresponds to variant rs35496743 [ dbSNP | Ensembl ].		VAR_052010
	Natural variant	204	1	R → Q. Corresponds to variant rs34758505 [ dbSNP | Ensembl ].		VAR_052011

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E44E76A40A5C2742	FASTA	699	79,789
	10 20 30 40 50 60 MKIAVLFCFF LLIIFQTDFG KNEEIPRKQR RKIYHRRLRK SSTSHKHRSN RQLGIQQTTV 70 80 90 100 110 120 FTPVARLPIV NFDYSMEEKF ESFSSFPGVE SSYNVLPGKK GHCLVKGITM YNKAVWSPEP 130 140 150 160 170 180 CTTCLCSDGR VLCDETMCHP QRCPQTVIPE GECCPVCSAT VSYSLLSGIA LNDRNEFSGD 190 200 210 220 230 240 SSEQREPTNL LHKQLPPPQV GMDRIVRKEA LQSEEDEEVK EEDTEQKRET PESRNQGQLY 250 260 270 280 290 300 SEGDSRGGDR KQRPGEERRL AHQQQRQGRE EEEDEEEEGE EGEEDEEDEE DPVRGDMFRM 310 320 330 340 350 360 PSRSPLPAPP RGTLRLPSGC SLSYRTISCI NAMLTQIPPL TAPQITSLEL TGNSIASIPD 370 380 390 400 410 420 EAFNGLPNLE RLDLSKNNIT SSGIGPKAFK LLKKLMRLNM DGNNLIQIPS QLPSTLEELK 430 440 450 460 470 480 VNENNLQAID EESLSDLNQL VTLELEGNNL SEANVNPLAF KPLKSLAYLR LGKNKFRIIP 490 500 510 520 530 540 QGLPGSIEEL YLENNQIEEI TEICFNHTRK INVIVLRYNK IEENRIAPLA WINQENLESI 550 560 570 580 590 600 DLSYNKLYHV PSYLPKSLLH LVLLGNQIER IPGYVFGHME PGLEYLYLSF NKLADDGMDR 610 620 630 640 650 660 VSFYGAYHSL RELFLDHNDL KSIPPGIQEM KALHFLRLNN NKIRNILPEE ICNAEEDDDS 670 680 690 NLEHLHLENN YIKIREIPSY TFSCIRSYSS IVLKPQNIK
	Isoform 2 [UniParc]. Checksum: 7F7C9722614F657A	FASTA	643	73,348
	10 20 30 40 50 60 MKIAVLFCFF LLIIFQTDFG KNEEIPRKQR RKIYHRRLRK SSTSHKHRSN RQLGIQQTTV 70 80 90 100 110 120 FTPVARLPIV NFDYSMEEKF ESFSSFPGVE SSYNVLPGKK GHCLVKGITM YNKAVWSPEP 130 140 150 160 170 180 CTTCLCSDGR VLCDETMCHP QRCPQTVIPE GECCPVCSAT EQREPTNLLH KQLPPPQVGM 190 200 210 220 230 240 DRIVRKEALQ SEEDEEVKEE DTEQKRETPE SRNQGQLYSE GDSRGGDRKQ RPGEERRLAH 250 260 270 280 290 300 QQQRQGREEE EDEEEEGEEG EEDEEDEEDP VRGDMFRMPS RSPLPAPPRG TLRLPSGCSL 310 320 330 340 350 360 SYRTISCINA MLTQIPPLTA PQITSLELTG NSIASIPDEA FNGLPNLERL DLSKNNITSS 370 380 390 400 410 420 GIGPKAFKLL KKLMRLNMDG NNLIQIPSQL PSTLEELKVN ENNLQAIDEE SLSDLNQLVT 430 440 450 460 470 480 LELEGNNLSE ANVNPLAFKP LKSLAYLRLG KNKFRIIPQG LPGSIEELYL ENNQIEEITE 490 500 510 520 530 540 ICFNHTRKIN VIVLRYNKIE ENRIAPLAWI NQENLESIDL SYNKLYHVPS YLPKSLLHLV 550 560 570 580 590 600 LLGNQIERIP GYVFGHMEPG LEYLYLSFNK LADDGMDRVS FYGAYHSLRE LFLDHNDLKS 610 620 630 640 IPPGIQEMKA LHFLRLNNNK IRCVSDAVLE TVTNRSDVAF PLW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel gene (ECM2) encoding a putative extracellular matrix protein expressed predominantly in adipose and female-specific tissues and its chromosomal localization to 9q22.3." Nishiu J., Tanaka T., Nakamura Y. Genomics 52:378-381(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-56. Tissue: Retina.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011792 mRNA. Translation: BAA33958.1. AK312820 mRNA. Translation: BAG35677.1. BX537976 mRNA. Translation: CAD97940.1. Different initiation. AL137848, AL157827 Genomic DNA. Translation: CAI16693.1. AL157827, AL137848 Genomic DNA. Translation: CAI12589.1. CH471089 Genomic DNA. Translation: EAW62824.1. BC107493 mRNA. Translation: AAI07494.1.
IPI	IPI00015315. IPI00967874.
RefSeq	NP_001184224.1. NM_001197295.1. NP_001184225.1. NM_001197296.1. NP_001384.1. NM_001393.3.
UniGene	Hs.117060.
3D structure databases
ProteinModelPortal	O94769.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4722311.
STRING	9606.ENSP00000344758.
PTM databases
PhosphoSite	O94769.
Proteomic databases
PaxDb	O94769.
PRIDE	O94769.
Protocols and materials databases
DNASU	1842.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000344604; ENSP00000344758; ENSG00000106823. ENST00000444490; ENSP00000393971; ENSG00000106823.
GeneID	1842.
KEGG	hsa:1842.
UCSC	uc004asf.4. human. uc011lty.2. human.
Organism-specific databases
CTD	1842.
GeneCards	GC09M095257.
HGNC	HGNC:3154. ECM2.
HPA	HPA035347.
MIM	603479. gene.
neXtProt	NX_O94769.
PharmGKB	PA27599.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4886.
HOGENOM	HOG000112319.
HOVERGEN	HBG031529.
InParanoid	O94769.
KO	K08119.
OMA	AVWSPKP.
OrthoDB	EOG4868CR.
PhylomeDB	O94769.
Gene expression databases
ArrayExpress	O94769.
Bgee	O94769.
CleanEx	HS_ECM2.
Genevestigator	O94769.
GermOnline	ENSG00000106823. Homo sapiens.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00093. VWC. 1 hit. [Graphical view]
SMART	SM00214. VWC. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 12 hits. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1842.
NextBio	7543.
SOURCE	Search...

Entry information

Entry name

ECM2_HUMAN

Accession

Primary (citable) accession number: O94769
Secondary accession number(s): B2R730 , E2PU11, Q5T9F2, Q7Z3D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	May 1, 1999
Last modified:	May 29, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.