Reviewed, UniProtKB/Swiss-Prot O94745 (MPPA_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable mitochondrial-processing peptidase subunit alpha
    EC=3.4.24.64
Alternative name(s):
    Alpha-MPP

Gene names

Name:	mas2
ORF Names:	SPBC18E5.12c, SPBC23G7.02c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors By similarity.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Subunit structure	Heterodimer of alpha and beta subunits By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M16 family.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 494		Probable mitochondrial-processing peptidase subunit alpha		PRO_0000026772

Sequences

	Sequence		Length	Mass (Da)
	O94745-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 714F2F4DBF03A276	FASTA	494	54,672
	10 20 30 40 50 60 MLSEYQCLKN IGFSHKTVLK RRLFRKECTP ALKSFYSTQD PALNEVRTEK LKNGVTYVCD 70 80 90 100 110 120 PRPGHFSGLG VYVKAGSRYE TKKFSGVSHF MDRLAFQATE RTPVGEMKAK LENLGGNYMC 130 140 150 160 170 180 STSRESMIYQ AAVFNDDVKS MSKLLAETVL APKIQEDDLV HYRDSIIYEN SELWTKPDAL 190 200 210 220 230 240 LGEFAHVTAF QNNTLGNCLL CTPDKVNGIT ATSIREYLKY FYRPEHLTLA YAGIPQEIAK 250 260 270 280 290 300 EITKELYGHL PSSSLPPLEA IPSHYTGGFM GIKKSEAPPV PYQQEFTHVV IAMEGLPVTD 310 320 330 340 350 360 PDIYALACLQ FLLGGGGSFS AGGPGKGMYS RLYLNVLNQY PWVETCMAFN HSYTDSGLFG 370 380 390 400 410 420 MFVTILDDAA HLAAPLIIRE LCNTVLSVTS EETERAKNQL KSSLLMNLES RMISLEDLGR 430 440 450 460 470 480 QIQTQNGLYI TPKEMIEKID ALTPSDLSRV ARRVLTGNVS NPGNGTGKPT VLIHGNVDEV 490 GDVFALCKKA GIGH

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA22672.1.
PIR	T39763.
RefSeq	NP_595859.1.
3D structure databases
HSSP	HSSP built from PDB template 1HR6 based on UniProtKB P11914.
ModBase	Search...
Protein family/group databases
MEROPS	M16.971.
Genome annotation databases
GeneID	2540713.
KEGG	spo:SPBC18E5.12c.
NMPDR	fig|4896.1.peg.1725.
Organism-specific databases
GeneDB_Spombe	SPBC18E5.12c.
Phylogenomic databases
OMA	O94745. YRGNTVG.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003745-MON.
BRENDA	3.4.24.64. 653.
Gene expression databases
ArrayExpress	O94745.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPA_SCHPO

Accession

Primary (citable) accession number: O94745

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)