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Protein

26S proteasome complex subunit SEM1

Gene

SEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Versatile protein that might stabilize multiple protein complexes involved in diverse pathways. Subunit of the 26S proteasome which plays a role in ubiquitin-dependent proteolysis. Associates also with the TREX-2 complex that is required for transcription-coupled mRNA export, and the COP9 signalosome, which is involved in deneddylation.2 Publications

Miscellaneous

Present with 5590 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • filamentous growth Source: SGD
  • histone deubiquitination Source: SGD
  • maintenance of DNA trinucleotide repeats Source: SGD
  • mRNA export from nucleus Source: SGD
  • proteasome assembly Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • regulation of cell cycle Source: SGD
  • SAGA complex localization to transcription regulatory region Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Biological processTranscription

Enzyme and pathway databases

BioCyciYEAST:G3O-30088-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome complex subunit SEM1
Gene namesi
Name:SEM1
Synonyms:DSH1
Ordered Locus Names:YDR363W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR363W-A
SGDiS000007235 SEM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

Pathology & Biotechi

Disruption phenotypei

Impairs mRNA export and transcription elongation, and induces strong transcription-associated hyperrecombination phenotypes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001229702 – 8926S proteasome complex subunit SEM1Add BLAST88

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO94742
PaxDbiO94742
PRIDEiO94742

PTM databases

iPTMnetiO94742

Interactioni

Subunit structurei

Part of the 26S proteasome. Associates with the nuclear pore complex (NPC)-associated TREX-2 complex and the COP9 signalosome. Interacts with CSN12 and THP3.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32420, 479 interactors
DIPiDIP-8754N
IntActiO94742, 18 interactors
MINTiO94742
STRINGi4932.YDR363W-A

Structurei

Secondary structure

189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi28 – 31Combined sources4
Beta strandi32 – 34Combined sources3
Beta strandi64 – 67Combined sources4
Helixi72 – 87Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCKelectron microscopy3.50I1-89[»]
3JCOelectron microscopy4.80Y1-89[»]
3JCPelectron microscopy4.60Y1-89[»]
3T5VX-ray2.90C/F1-89[»]
4CR2electron microscopy7.70Y1-89[»]
4CR3electron microscopy9.30Y1-89[»]
4CR4electron microscopy8.80Y1-89[»]
4TRQX-ray3.10C/F30-89[»]
5A5Belectron microscopy9.50Y1-89[»]
5G5Pelectron microscopy5.30C1-89[»]
5L3TX-ray4.93C1-89[»]
5MPBelectron microscopy7.80Y1-89[»]
5MPCelectron microscopy7.70Y1-89[»]
5MPDelectron microscopy4.10Y1-89[»]
5MPEelectron microscopy4.50Y1-89[»]
5UBPX-ray2.30C1-89[»]
5WVIelectron microscopy6.30Y1-89[»]
5WVKelectron microscopy4.20Y1-89[»]
DisProtiDP01014
ProteinModelPortaliO94742
SMRiO94742
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DSS1/SEM1 family.Curated

Phylogenomic databases

InParanoidiO94742
KOiK10881
OMAiSTQHLWE

Family and domain databases

InterProiView protein in InterPro
IPR007834 DSS1_SEM1
PANTHERiPTHR16771 PTHR16771, 1 hit
PfamiView protein in Pfam
PF05160 DSS1_SEM1, 1 hit
SMARTiView protein in SMART
SM01385 DSS1_SEM1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O94742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDVAAAQA QSKIDLTKKK NEEINKKSLE EDDEFEDFPI DTWANGETIK
60 70 80
SNAVTQTNIW EENWDDVEVD DDFTNELKAE LDRYKRENQ
Length:89
Mass (Da):10,386
Last modified:May 1, 1999 - v1
Checksum:iB66B578ABB01E672
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059310 mRNA Translation: AAD08804.1
AF065136 Genomic DNA Translation: AAC96096.1
U28372 Genomic DNA No translation available.
AY557719 Genomic DNA Translation: AAS56045.1
BK006938 Genomic DNA Translation: DAA12202.1
PIRiS78744
RefSeqiNP_010651.3, NM_001184328.3

Genome annotation databases

EnsemblFungiiYDR363W-A; YDR363W-A; YDR363W-A
GeneIDi851967
KEGGisce:YDR363W-A

Similar proteinsi

Entry informationi

Entry nameiSEM1_YEAST
AccessioniPrimary (citable) accession number: O94742
Secondary accession number(s): D6VSZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health