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Protein

26S proteasome complex subunit SEM1

Gene

SEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Versatile protein that might stabilize multiple protein complexes involved in diverse pathways. Subunit of the 26S proteasome which plays a role in ubiquitin-dependent proteolysis. Associates also with the TREX-2 complex that is required for transcription-coupled mRNA export, and the COP9 signalosome, which is involved in deneddylation.2 Publications

GO - Biological processi

  • double-strand break repair via homologous recombination Source: GO_Central
  • exocytosis Source: SGD
  • filamentous growth Source: SGD
  • histone deubiquitination Source: SGD
  • maintenance of DNA trinucleotide repeats Source: SGD
  • mRNA export from nucleus Source: SGD
  • proteasome assembly Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • regulation of cell cycle Source: SGD
  • SAGA complex localization to transcription regulatory region Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-30088-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome complex subunit SEM1
Gene namesi
Name:SEM1
Synonyms:DSH1
Ordered Locus Names:YDR363W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR363W-A.
SGDiS000007235. SEM1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
  • proteasome regulatory particle, lid subcomplex Source: SGD
  • proteasome storage granule Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Proteasome

Pathology & Biotechi

Disruption phenotypei

Impairs mRNA export and transcription elongation, and induces strong transcription-associated hyperrecombination phenotypes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001229702 – 8926S proteasome complex subunit SEM1Add BLAST88

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO94742.
PRIDEiO94742.

PTM databases

iPTMnetiO94742.

Interactioni

Subunit structurei

Part of the 26S proteasome. Associates with the nuclear pore complex (NPC)-associated TREX-2 complex and the COP9 signalosome. Interacts with CSN12 and THP3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSM3P383482EBI-31337,EBI-21152

Protein-protein interaction databases

BioGridi32420. 353 interactors.
DIPiDIP-8754N.
IntActiO94742. 15 interactors.
MINTiMINT-2779426.

Structurei

Secondary structure

189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni62 – 66Combined sources5
Helixi72 – 87Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCKelectron microscopy3.50I1-89[»]
3JCOelectron microscopy4.80Y1-89[»]
3JCPelectron microscopy4.60Y1-89[»]
3T5VX-ray2.90C/F1-89[»]
4CR2electron microscopy7.70Y1-89[»]
4CR3electron microscopy9.30Y1-89[»]
4CR4electron microscopy8.80Y1-89[»]
4TRQX-ray3.10C/F30-89[»]
5A5Belectron microscopy9.50Y1-89[»]
5L3TX-ray4.93C1-89[»]
ProteinModelPortaliO94742.
SMRiO94742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DSS1/SEM1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018216.
InParanoidiO94742.
KOiK10881.
OMAiIWEENWD.

Family and domain databases

InterProiIPR007834. DSS1_SEM1.
[Graphical view]
PfamiPF05160. DSS1_SEM1. 1 hit.
[Graphical view]
SMARTiSM01385. DSS1_SEM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O94742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDVAAAQA QSKIDLTKKK NEEINKKSLE EDDEFEDFPI DTWANGETIK
60 70 80
SNAVTQTNIW EENWDDVEVD DDFTNELKAE LDRYKRENQ
Length:89
Mass (Da):10,386
Last modified:May 1, 1999 - v1
Checksum:iB66B578ABB01E672
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059310 mRNA. Translation: AAD08804.1.
AF065136 Genomic DNA. Translation: AAC96096.1.
U28372 Genomic DNA. No translation available.
AY557719 Genomic DNA. Translation: AAS56045.1.
BK006938 Genomic DNA. Translation: DAA12202.1.
PIRiS78744.
RefSeqiNP_010651.3. NM_001184328.3.

Genome annotation databases

EnsemblFungiiYDR363W-A; YDR363W-A; YDR363W-A.
GeneIDi851967.
KEGGisce:YDR363W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059310 mRNA. Translation: AAD08804.1.
AF065136 Genomic DNA. Translation: AAC96096.1.
U28372 Genomic DNA. No translation available.
AY557719 Genomic DNA. Translation: AAS56045.1.
BK006938 Genomic DNA. Translation: DAA12202.1.
PIRiS78744.
RefSeqiNP_010651.3. NM_001184328.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCKelectron microscopy3.50I1-89[»]
3JCOelectron microscopy4.80Y1-89[»]
3JCPelectron microscopy4.60Y1-89[»]
3T5VX-ray2.90C/F1-89[»]
4CR2electron microscopy7.70Y1-89[»]
4CR3electron microscopy9.30Y1-89[»]
4CR4electron microscopy8.80Y1-89[»]
4TRQX-ray3.10C/F30-89[»]
5A5Belectron microscopy9.50Y1-89[»]
5L3TX-ray4.93C1-89[»]
ProteinModelPortaliO94742.
SMRiO94742.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32420. 353 interactors.
DIPiDIP-8754N.
IntActiO94742. 15 interactors.
MINTiMINT-2779426.

PTM databases

iPTMnetiO94742.

Proteomic databases

MaxQBiO94742.
PRIDEiO94742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR363W-A; YDR363W-A; YDR363W-A.
GeneIDi851967.
KEGGisce:YDR363W-A.

Organism-specific databases

EuPathDBiFungiDB:YDR363W-A.
SGDiS000007235. SEM1.

Phylogenomic databases

GeneTreeiENSGT00390000018216.
InParanoidiO94742.
KOiK10881.
OMAiIWEENWD.

Enzyme and pathway databases

BioCyciYEAST:G3O-30088-MONOMER.

Miscellaneous databases

PROiO94742.

Family and domain databases

InterProiIPR007834. DSS1_SEM1.
[Graphical view]
PfamiPF05160. DSS1_SEM1. 1 hit.
[Graphical view]
SMARTiSM01385. DSS1_SEM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEM1_YEAST
AccessioniPrimary (citable) accession number: O94742
Secondary accession number(s): D6VSZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.