Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-1,3/1,6-mannosyltransferase ALG2

Gene

ALG2

Organism
Rhizomucor pusillus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.

Catalytic activityi

GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.
GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3/1,6-mannosyltransferase ALG2 (EC:2.4.1.132, EC:2.4.1.257)
Alternative name(s):
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene namesi
Name:ALG2
OrganismiRhizomucor pusillus
Taxonomic identifieri4840 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotinaMucoralesLichtheimiaceaeRhizomucor

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei73 – 9523HelicalSequence analysisAdd
BLAST
Transmembranei434 – 45320HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi368 – 3681G → R: Temperature-sensitive phenotype. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455Alpha-1,3/1,6-mannosyltransferase ALG2PRO_0000080268Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliO94738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKSLNVAF IHPDLGIGGA ERLVVDAAVG IQKKGHQVIF YTSHHDPNHC
60 70 80 90 100
FEETRDGTLK VQVRGDWLPR TIFGRFYILC AILRQFVLVA SLILWERHSY
110 120 130 140 150
DIFFVDQLSA CVPLLKWFTT AKILFYCHFP DKLLTQRNST IKKLYRAPVD
160 170 180 190 200
KMEELTTGMS DLIAVNSGFT AGMFKKSFPS VHQTPQILYP PINFDAYDRP
210 220 230 240 250
VDRNDPTVKI LETDKRVLLS INRFERKKNV ELALRAFAAL KIKNMVPKDV
260 270 280 290 300
FANYRLVLAG GYDKRVRENV EYLEELDQLA TEEFGLQTFT IHPSSAAADV
310 320 330 340 350
PADAQVVFLC SFNDAQRTFL LDQAKLLLYT PSNEHFGITP VEGMYASVPV
360 370 380 390 400
IAVNTGGPVE TVKNKETGLL LPSDPDVWAE GIRDFIIEKY NGKQMGQHGR
410 420 430 440 450
QHVQSKFSLP AFADRLEAMM IELETSTPDQ SSSGAVYLLG AIGVLFACII

YCIKQ
Length:455
Mass (Da):51,222
Last modified:May 1, 1999 - v1
Checksum:i8128C7C2365E46EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015055 mRNA. Translation: BAA34297.1.
AB015054 Genomic DNA. Translation: BAA34296.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015055 mRNA. Translation: BAA34297.1.
AB015054 Genomic DNA. Translation: BAA34296.1.

3D structure databases

ProteinModelPortaliO94738.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of alg2 encoding a mannosyltransferase in the zygomycete fungus Rhizomucor pusillus."
    Yamazaki H., Shiraishi N., Takeuchi K., Ohnishi Y., Horinouchi S.
    Gene 221:179-184(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF GLY-368.

Entry informationi

Entry nameiALG2_RHIPU
AccessioniPrimary (citable) accession number: O94738
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 1999
Last modified: May 11, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.