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O94737

- MAPK1_PNECA

UniProt

O94737 - MAPK1_PNECA

Protein

Mitogen-activated protein kinase 1

Gene

MKP1

Organism
Pneumocystis carinii
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Serine-threonine protein kinase which may be involved in maintenance of cell integrity. Functionally complements SLT2 null mutant in S.cerevisiae.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation. Activated by oxidative stress.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521ATPPROSITE-ProRule annotation
    Active sitei149 – 1491Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 359ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent protein binding Source: UniProtKB
    3. MAP kinase activity Source: UniProtKB

    GO - Biological processi

    1. cellular component organization Source: UniProtKB
    2. MAPK cascade Source: UniProtKB
    3. positive regulation of growth rate Source: UniProtKB
    4. response to oxidative stress Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 1 (EC:2.7.11.24)
    Alternative name(s):
    MAP kinase Mkp1
    Gene namesi
    Name:MKP1Imported
    OrganismiPneumocystis carinii
    Taxonomic identifieri4754 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaPneumocystidomycetesPneumocystidaceaePneumocystis

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi52 – 521K → R: Loss of kinase activity. 2 Publications
    Mutagenesisi182 – 1821T → A: No change in activity; when associated with F-184. 1 Publication
    Mutagenesisi184 – 1841Y → F: No change in activity; when associated with A-182. 1 Publication
    Mutagenesisi186 – 1861T → A: Loss of kinase activity; when associated with F-188. 2 Publications
    Mutagenesisi188 – 1881Y → F: Loss of kinase activity; when associated with A-186. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Mitogen-activated protein kinase 1PRO_0000247743Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei186 – 1861Phosphothreonine
    Modified residuei188 – 1881Phosphotyrosine

    Post-translational modificationi

    Dually phosphorylated on Thr-186 and Tyr-188, which activates the enzyme.

    Keywords - PTMi

    Phosphoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliO94737.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 314294Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi186 – 1883TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94737-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSKHSVFNV MGQEFVLQKG YEVIKGLGKG SYGVVCSAKN IEVEDNNKVA    50
    IKKITNIFSK KMLAKRALRE IMLLQHFRNH KNITTLYDMD IVDPSKFNEL 100
    YLYEELMQAN LNSIIRSDQP LTDAHFQSFI YQILCGLKYI HSANVLHRDL 150
    KPSNLLINAD CKLKICDFGL SRGISVNQGQ GTEYMTEYVT TRWYRAPEVM 200
    LSFQSYSKAI DLWSVGCILA ELLGRKPFFK GSNYVDQLNQ IFCILGTPNE 250
    NIISKIKSAS AQSYIRSLPT LPKMPYSKIF PYANPDALDL LNCLLTFDPY 300
    DRISCEEALE HPYLIIWHDP NKEPVCSEQF DFGFEAIDSI EEIRQMIINE 350
    VSRFKGLTGN QKLYHSISNT 370
    Length:370
    Mass (Da):42,396
    Last modified:May 1, 1999 - v1
    Checksum:i7DE7E10B8A06536E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077677 mRNA. Translation: AAD10000.1.
    AF084383 Genomic DNA. Translation: AAD16043.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077677 mRNA. Translation: AAD10000.1 .
    AF084383 Genomic DNA. Translation: AAD16043.1 .

    3D structure databases

    ProteinModelPortali O94737.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitogen-activated protein kinase Mkp1 of Pneumocystis carinii complements the slt2Delta defect in the cell integrity pathway of Saccharomyces cerevisiae."
      Fox D., Smulian A.G.
      Mol. Microbiol. 34:451-462(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF LYS-52; THR-186 AND TYR-188.
    2. "Mkp1 of Pneumocystis carinii associates with the yeast transcription factor Rlm1 via a mechanism independent of the activation state."
      Fox D., Smulian A.G.
      Cell. Signal. 12:381-390(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LYS-52; THR-182; TYR-184; THR-186 AND TYR-188.

    Entry informationi

    Entry nameiMAPK1_PNECA
    AccessioniPrimary (citable) accession number: O94737
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3