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O94737 (MAPK1_PNECA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 1

EC=2.7.11.24
Alternative name(s):
MAP kinase Mkp1
Gene names
Name:MKP1
OrganismPneumocystis carinii
Taxonomic identifier4754 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaPneumocystidomycetesPneumocystidaceaePneumocystis

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine-threonine protein kinase which may be involved in maintenance of cell integrity. Functionally complements SLT2 null mutant in S.cerevisiae. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1

Enzyme regulation

Activated by threonine and tyrosine phosphorylation. Activated by oxidative stress. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-186 and Tyr-188, which activates the enzyme.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Mitogen-activated protein kinase 1
PRO_0000247743

Regions

Domain21 – 314294Protein kinase
Nucleotide binding27 – 359ATP By similarity
Motif186 – 1883TXY

Sites

Active site1491Proton acceptor By similarity
Binding site521ATP By similarity

Amino acid modifications

Modified residue1861Phosphothreonine Ref.1
Modified residue1881Phosphotyrosine Ref.1

Experimental info

Mutagenesis521K → R: Loss of kinase activity. Ref.1 Ref.2
Mutagenesis1821T → A: No change in activity; when associated with F-184. Ref.2
Mutagenesis1841Y → F: No change in activity; when associated with A-182. Ref.2
Mutagenesis1861T → A: Loss of kinase activity; when associated with F-188. Ref.1 Ref.2
Mutagenesis1881Y → F: Loss of kinase activity; when associated with A-186. Ref.1 Ref.2

Sequences

Sequence LengthMass (Da)Tools
O94737 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 7DE7E10B8A06536E

FASTA37042,396
        10         20         30         40         50         60 
MDSKHSVFNV MGQEFVLQKG YEVIKGLGKG SYGVVCSAKN IEVEDNNKVA IKKITNIFSK 

        70         80         90        100        110        120 
KMLAKRALRE IMLLQHFRNH KNITTLYDMD IVDPSKFNEL YLYEELMQAN LNSIIRSDQP 

       130        140        150        160        170        180 
LTDAHFQSFI YQILCGLKYI HSANVLHRDL KPSNLLINAD CKLKICDFGL SRGISVNQGQ 

       190        200        210        220        230        240 
GTEYMTEYVT TRWYRAPEVM LSFQSYSKAI DLWSVGCILA ELLGRKPFFK GSNYVDQLNQ 

       250        260        270        280        290        300 
IFCILGTPNE NIISKIKSAS AQSYIRSLPT LPKMPYSKIF PYANPDALDL LNCLLTFDPY 

       310        320        330        340        350        360 
DRISCEEALE HPYLIIWHDP NKEPVCSEQF DFGFEAIDSI EEIRQMIINE VSRFKGLTGN 

       370 
QKLYHSISNT 

« Hide

References

[1]"Mitogen-activated protein kinase Mkp1 of Pneumocystis carinii complements the slt2Delta defect in the cell integrity pathway of Saccharomyces cerevisiae."
Fox D., Smulian A.G.
Mol. Microbiol. 34:451-462(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF LYS-52; THR-186 AND TYR-188.
[2]"Mkp1 of Pneumocystis carinii associates with the yeast transcription factor Rlm1 via a mechanism independent of the activation state."
Fox D., Smulian A.G.
Cell. Signal. 12:381-390(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF LYS-52; THR-182; TYR-184; THR-186 AND TYR-188.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF077677 mRNA. Translation: AAD10000.1.
AF084383 Genomic DNA. Translation: AAD16043.1.

3D structure databases

ProteinModelPortalO94737.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAPK1_PNECA
AccessionPrimary (citable) accession number: O94737
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families