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O94737

- MAPK1_PNECA

UniProt

O94737 - MAPK1_PNECA

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Protein

Mitogen-activated protein kinase 1

Gene

MKP1

Organism
Pneumocystis carinii
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine-threonine protein kinase which may be involved in maintenance of cell integrity. Functionally complements SLT2 null mutant in S.cerevisiae.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation. Activated by oxidative stress.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATPPROSITE-ProRule annotation
Active sitei149 – 1491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 359ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent protein binding Source: UniProtKB
  3. MAP kinase activity Source: UniProtKB

GO - Biological processi

  1. cellular component organization Source: UniProtKB
  2. MAPK cascade Source: UniProtKB
  3. positive regulation of growth rate Source: UniProtKB
  4. response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 1 (EC:2.7.11.24)
Alternative name(s):
MAP kinase Mkp1
Gene namesi
Name:MKP1Imported
OrganismiPneumocystis carinii
Taxonomic identifieri4754 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaPneumocystidomycetesPneumocystidaceaePneumocystis

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 521K → R: Loss of kinase activity. 2 Publications
Mutagenesisi182 – 1821T → A: No change in activity; when associated with F-184. 1 Publication
Mutagenesisi184 – 1841Y → F: No change in activity; when associated with A-182. 1 Publication
Mutagenesisi186 – 1861T → A: Loss of kinase activity; when associated with F-188. 2 Publications
Mutagenesisi188 – 1881Y → F: Loss of kinase activity; when associated with A-186. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Mitogen-activated protein kinase 1PRO_0000247743Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei186 – 1861Phosphothreonine
Modified residuei188 – 1881Phosphotyrosine

Post-translational modificationi

Dually phosphorylated on Thr-186 and Tyr-188, which activates the enzyme.

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliO94737.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 314294Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi186 – 1883TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94737-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSKHSVFNV MGQEFVLQKG YEVIKGLGKG SYGVVCSAKN IEVEDNNKVA
60 70 80 90 100
IKKITNIFSK KMLAKRALRE IMLLQHFRNH KNITTLYDMD IVDPSKFNEL
110 120 130 140 150
YLYEELMQAN LNSIIRSDQP LTDAHFQSFI YQILCGLKYI HSANVLHRDL
160 170 180 190 200
KPSNLLINAD CKLKICDFGL SRGISVNQGQ GTEYMTEYVT TRWYRAPEVM
210 220 230 240 250
LSFQSYSKAI DLWSVGCILA ELLGRKPFFK GSNYVDQLNQ IFCILGTPNE
260 270 280 290 300
NIISKIKSAS AQSYIRSLPT LPKMPYSKIF PYANPDALDL LNCLLTFDPY
310 320 330 340 350
DRISCEEALE HPYLIIWHDP NKEPVCSEQF DFGFEAIDSI EEIRQMIINE
360 370
VSRFKGLTGN QKLYHSISNT
Length:370
Mass (Da):42,396
Last modified:May 1, 1999 - v1
Checksum:i7DE7E10B8A06536E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077677 mRNA. Translation: AAD10000.1.
AF084383 Genomic DNA. Translation: AAD16043.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077677 mRNA. Translation: AAD10000.1 .
AF084383 Genomic DNA. Translation: AAD16043.1 .

3D structure databases

ProteinModelPortali O94737.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mitogen-activated protein kinase Mkp1 of Pneumocystis carinii complements the slt2Delta defect in the cell integrity pathway of Saccharomyces cerevisiae."
    Fox D., Smulian A.G.
    Mol. Microbiol. 34:451-462(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF LYS-52; THR-186 AND TYR-188.
  2. "Mkp1 of Pneumocystis carinii associates with the yeast transcription factor Rlm1 via a mechanism independent of the activation state."
    Fox D., Smulian A.G.
    Cell. Signal. 12:381-390(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-52; THR-182; TYR-184; THR-186 AND TYR-188.

Entry informationi

Entry nameiMAPK1_PNECA
AccessioniPrimary (citable) accession number: O94737
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3