Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ferric/cupric reductase transmembrane component 2

Gene

frp2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Metalloreductase responsible for reducing extracellular iron and copper prior to import (By similarity). Catalyzes the reductive uptake of Fe3+-salts and Fe3+ bound to catecholate or hydroxamate siderophores (By similarity). Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane (By similarity). Also participates in Cu2+ reduction and Cu+ uptake (By similarity).By similarity

Catalytic activityi

2 Fe(II)-siderophore + NADP+ + H+ = 2 Fe(III)-siderophore + NADPH.By similarity

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarity
  • hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi150Iron (heme 1 axial ligand)By similarity1
Metal bindingi164Iron (heme 2 axial ligand)By similarity1
Metal bindingi218Iron (heme 1 axial ligand)By similarity1
Metal bindingi232Iron (heme 2 axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi310 – 316FADSequence analysis7
Nucleotide bindingi419 – 427NADSequence analysis9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Ion transport, Iron transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric/cupric reductase transmembrane component 2Curated (EC:1.16.1.9By similarity)
Alternative name(s):
Ferric-chelate reductase 2Curated
Gene namesi
Name:frp2
ORF Names:SPBC947.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC947.05c
PomBaseiSPBC947.05c frp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Transmembranei184 – 204HelicalSequence analysisAdd BLAST21
Transmembranei210 – 230HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003372601 – 564Ferric/cupric reductase transmembrane component 2Add BLAST564

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi350N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi442N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi496N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi548N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO94727
PaxDbiO94727
PRIDEiO94727

PTM databases

SwissPalmiO94727

Interactioni

Protein-protein interaction databases

BioGridi277741, 14 interactors
STRINGi4896.SPBC947.05c.1

Structurei

3D structure databases

ProteinModelPortaliO94727
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 229Ferric oxidoreductaseSequence analysisAdd BLAST116
Domaini254 – 410FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST157

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000112645
InParanoidiO94727
OMAiLDSYGHA
OrthoDBiEOG092C2JFA
PhylomeDBiO94727

Family and domain databases

InterProiView protein in InterPro
IPR000778 Cyt_b245_heavy_chain
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
PRINTSiPR00466 GP91PHOX
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

O94727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILARDDKWT LGSIALIFVL LIGFALLFLL ERFRVKEKSR TFKDCVNVYQ
60 70 80 90 100
CPSKGERVYL ALRHWFIFLA THKAQMTLIL SPLVMLVTIP FTGKETKNSI
110 120 130 140 150
ASYDWNLTGV AARLGYLSCG LFFVSYFFSL KNNPFCLMLF SSHEKMNYLH
160 170 180 190 200
RWLSVYAVLI SVLHGILFMI FSAQSYKPLL YDKISIYGYF ITVVLFLMTV
210 220 230 240 250
ASLPSVRRKF FEWFFVLHHT CSVLIIFLIW LHHPRTIVYM KACIIIYAFD
260 270 280 290 300
RGCRLFRSIW NRSNFRIYLL NEDMIYMVGR KPKRSFFALP WAAGSHVYIN
310 320 330 340 350
IPSLSYWQVH PFTLASAPFD DFIELFVAVH SGFTERLANR LYSMPHEYPN
360 370 380 390 400
FSLAPGTPES LSNTYRELNS FKSYAVEIEN TAQGHTYEPE DLYLETTVFM
410 420 430 440 450
DGPYGTTSNV FKEYSYVLLI AGGVGFSYTL PILRDLILKE CNVTSITFIW
460 470 480 490 500
SCRSLSLLKV ASKSLNSLLH QSNVRLKIIN HFTGSISCKE SSEFSNQTTE
510 520 530 540 550
NSEMEFFDDR PDLDMYIQKF FDYVGYQTAA LAACGSQSFL KRIKNSVNKS
560
ISSTTDIYQH YEEL
Length:564
Mass (Da):65,262
Last modified:May 1, 1999 - v1
Checksum:i8890551FB5AAFD40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA17033.1
PIRiT40777
RefSeqiNP_595271.1, NM_001021178.1

Genome annotation databases

EnsemblFungiiSPBC947.05c.1; SPBC947.05c.1:pep; SPBC947.05c
GeneIDi2541227
KEGGispo:SPBC947.05c

Entry informationi

Entry nameiFRP2_SCHPO
AccessioniPrimary (citable) accession number: O94727
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health