O94688 (APC15_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaphase-promoting complex subunit 15

Alternative name(s):
20S cyclosome/APC complex protein apc15

Gene names

Name:	apc15
ORF Names:	SPBC83.04

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. Ref.2
Subunit structure	The APC/C is composed of at least 13 subunits: apc1, apc2, nuc2, apc4, apc5, cut9, apc8, apc10, apc11, hcn1, apc13, apc14 and apc15. Ref.2
Sequence similarities	Belongs to the APC15 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Traceable author statement Ref.2. Source: PomBase regulation of mitotic metaphase/anaphase transition Inferred by curator. Source: PomBase
Cellular_component	anaphase-promoting complex Inferred from direct assay Ref.2. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
apc13	O74358	3	EBI-1251604,EBI-1251583
apc14	O42659	2	EBI-1251604,EBI-1251592
cut9	P41889	2	EBI-1251604,EBI-1160859

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 136	136	Anaphase-promoting complex subunit 15		PRO_0000237685

Sequences

	Sequence		Length	Mass (Da)
	O94688 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 086B97F02A6F0C8B	FASTA	136	15,786
	10 20 30 40 50 60 MSFMSLMANT AHQLWYPTSF PSMKELEKEE VRLETQELAI KQFGFRTIRP IGLNRSMQEQ 70 80 90 100 110 120 LDLEEQEREE ANQDTELDEE ELGSGSFPEE GEMDDMDGDN EEVEDHDIEE VDLDADITNA 130 DASEFYEDIS EYGFQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes." Yoon H.-J., Feoktistova A., Wolfe B.A., Jennings J.L., Link A.J., Gould K.L. Curr. Biol. 12:2048-2054(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB36866.1.
PIR	T40693.
RefSeq	NP_595636.1. NM_001021530.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O94688. 4 interactions.
STRING	4896.SPBC83.04-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC83.04.1; SPBC83.04.1:pep; SPBC83.04.
GeneID	2540655.
KEGG	spo:SPBC83.04.
Organism-specific databases
PomBase	SPBC83.04.
Phylogenomic databases
OrthoDB	EOG48SM4N.
Family and domain databases
InterPro	IPR008402. APC_su15/MND2. [Graphical view]
Pfam	PF05841. Apc15p. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801779.

Entry information

Entry name

APC15_SCHPO

Accession

Primary (citable) accession number: O94688

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program