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Protein

Phospholipid:diacylglycerol acyltransferase

Gene

plh1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Triacylglycerol formation by an acyl-CoA independent pathway. The enzyme specifically transfers acyl groups from the sn-2 position of a phospholipid to diacylglycerol, thus forming an sn-1-lysophospholipid. Plays a major role in triacylglycerol formation at log phase. Cells defective in triacylglycerol synthesis undergo apoptosis upon entry into the stationary phase. Generation of reactive oxygen species (ROS) is essential to lipoapoptosis.1 Publication

Catalytic activityi

Phospholipid + 1,2-diacylglycerol = lysophospholipid + triacylglycerol.1 Publication

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei293 – 2931Acyl-ester intermediateSequence analysis
Active sitei535 – 5351Charge relay systemBy similarity
Active sitei586 – 5861Charge relay systemBy similarity

GO - Molecular functioni

  • O-acyltransferase activity Source: InterPro
  • phospholipid:diacylglycerol acyltransferase activity Source: PomBase

GO - Biological processi

  • diacylglycerol metabolic process Source: PomBase
  • lipid storage Source: PomBase
  • triglyceride biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00282.

Protein family/group databases

ESTHERischpo-pdat. PC-sterol_acyltransferase.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid:diacylglycerol acyltransferase (EC:2.3.1.158)
Short name:
PDAT
Alternative name(s):
Pombe LRO1 homolog 1
Gene namesi
Name:plh1
ORF Names:SPBC776.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC776.14.
PomBaseiSPBC776.14. plh1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5656CytoplasmicSequence analysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence analysisAdd
BLAST
Topological domaini78 – 632555LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 632632Phospholipid:diacylglycerol acyltransferasePRO_0000058267Add
BLAST

Proteomic databases

MaxQBiO94680.

Interactioni

Protein-protein interaction databases

BioGridi277701. 24 interactions.
MINTiMINT-4686137.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 144Poly-Lys

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000196602.
InParanoidiO94680.
KOiK00679.
OMAiWAPDDQP.
OrthoDBiEOG092C115H.

Family and domain databases

Gene3Di3.40.50.1820. 4 hits.
InterProiIPR029058. AB_hydrolase.
IPR003386. LACT/PDAT_acylTrfase.
[Graphical view]
PfamiPF02450. LCAT. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 3 hits.

Sequencei

Sequence statusi: Complete.

O94680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSKKSKTH KKKKEVKSPI DLPNSKKPTR ALSEQPSASE TQSVSNKSRK
60 70 80 90 100
SKFGKRLNFI LGAILGICGA FFFAVGDDNA VFDPATLDKF GNMLGSSDLF
110 120 130 140 150
DDIKGYLSYN VFKDAPFTTD KPSQSPSGNE VQVGLDMYNE GYRSDHPVIM
160 170 180 190 200
VPGVISSGLE SWSFNNCSIP YFRKRLWGSW SMLKAMFLDK QCWLEHLMLD
210 220 230 240 250
KKTGLDPKGI KLRAAQGFEA ADFFITGYWI WSKVIENLAA IGYEPNNMLS
260 270 280 290 300
ASYDWRLSYA NLEERDKYFS KLKMFIEYSN IVHKKKVVLI SHSMGSQVTY
310 320 330 340 350
YFFKWVEAEG YGNGGPTWVN DHIEAFINIS GSLIGAPKTV AALLSGEMKD
360 370 380 390 400
TAQLNQFSVY GLEKFFSRSE RAMMVRTMGG VSSMLPKGGD VVWGNASWAP
410 420 430 440 450
DDLNQTNFSN GAIIRYREDI DKDHDEFDID DALQFLKNVT DDDFKVMLAK
460 470 480 490 500
NYSHGLAWTE KEVLKNNEMP SKWINPLETS LPYAPDMKIY CVHGVGKPTE
510 520 530 540 550
RGYYYTNNPE GQPVIDSSVN DGTKVENGIV MDDGDGTLPI LALGLVCNKV
560 570 580 590 600
WQTKRFNPAN TSITNYEIKH EPAAFDLRGG PRSAEHVDIL GHSELNEIIL
610 620 630
KVSSGHGDSV PNRYISDIQE IINEINLDKP RN
Length:632
Mass (Da):70,833
Last modified:October 3, 2012 - v2
Checksum:iD7F2D81FDA76EDE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22887.2.
PIRiT40685.
RefSeqiNP_596330.2. NM_001022251.2.

Genome annotation databases

EnsemblFungiiSPBC776.14.1; SPBC776.14.1:pep; SPBC776.14.
GeneIDi2541187.
KEGGispo:SPBC776.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22887.2.
PIRiT40685.
RefSeqiNP_596330.2. NM_001022251.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277701. 24 interactions.
MINTiMINT-4686137.

Protein family/group databases

ESTHERischpo-pdat. PC-sterol_acyltransferase.

Proteomic databases

MaxQBiO94680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC776.14.1; SPBC776.14.1:pep; SPBC776.14.
GeneIDi2541187.
KEGGispo:SPBC776.14.

Organism-specific databases

EuPathDBiFungiDB:SPBC776.14.
PomBaseiSPBC776.14. plh1.

Phylogenomic databases

HOGENOMiHOG000196602.
InParanoidiO94680.
KOiK00679.
OMAiWAPDDQP.
OrthoDBiEOG092C115H.

Enzyme and pathway databases

UniPathwayiUPA00282.

Miscellaneous databases

PROiO94680.

Family and domain databases

Gene3Di3.40.50.1820. 4 hits.
InterProiIPR029058. AB_hydrolase.
IPR003386. LACT/PDAT_acylTrfase.
[Graphical view]
PfamiPF02450. LCAT. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPDAT_SCHPO
AccessioniPrimary (citable) accession number: O94680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: October 3, 2012
Last modified: September 7, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.