Reviewed, UniProtKB/Swiss-Prot O94671 (DHOM_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable homoserine dehydrogenase
      Short name=HDH
    EC=1.1.1.3

Gene names

ORF Names:

SPBC776.03

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Sequence similarities	Belongs to the homoserine dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Probable homoserine dehydrogenase		PRO_0000066706
Regions
	Nucleotide binding	13 – 20	8	NADP By similarity
Sites
	Active site	231	1	Proton donor Potential
	Binding site	99	1	NADP By similarity
	Binding site	123	1	NADP By similarity
	Binding site	216	1	Substrate By similarity
Amino acid modifications
	Modified residue	201	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O94671-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3A2BC3A2CBA9263C	FASTA	376	40,037
	10 20 30 40 50 60 MSASRTNVNV AIVGTGNIGG ELLNQIKGFN ENASTNGTTS FNVVAISSME GHYVSKDYQP 70 80 90 100 110 120 INLSEWKNLT SQNQGAYSLD ALVDFLAKSP LPAILVDNTA SEAIAQSYPK FLSKKINIAT 130 140 150 160 170 180 PNKKAFSASN DVYQNIIKAS KESGALLMHE ASVGAGLPII STLKELIATG DEIIKIEGIF 190 200 210 220 230 240 SGTLSYIFNV WSPNGKKGTA SFSDIVKIAK QNGYTEPDPR DDLNGMDVAR KVTILSRIAG 250 260 270 280 290 300 VHVESASSFP VKSLIPEPLK SAVNAEEFLA GLPNFDSEFA SMREEAEKEG KVVRFVGEAD 310 320 330 340 350 360 VANKTTLVKL EKYDASHPFA NLQSSDNIIS FTTKRYHTRP LVVIGAGAGA AVTAAGVLGD 370 MIKIMSQVRA SDAPSA

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA22876.1.
PIR	T40673.
RefSeq	NP_596318.1.
3D structure databases
HSSP	HSSP built from PDB template 1EBF based on UniProtKB P31116.
ModBase	Search...
Protein-protein interaction databases
STRING	O94671.
Genome annotation databases
GeneID	2541148.
KEGG	spo:SPBC776.03.
NMPDR	fig|4896.1.peg.2184.
Organism-specific databases
GeneDB_Spombe	SPBC776.03.
Phylogenomic databases
OMA	PNKKAFS.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004336-MON.
BRENDA	1.1.1.3. 653.
Gene expression databases
ArrayExpress	O94671.
Family and domain databases
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR001342. Homoserine_dehydrogenase_cat. IPR019811. Homoserine_dehydrogenase_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PROSITE	PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHOM_SCHPO

Accession

Primary (citable) accession number: O94671

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)