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Protein

Probable homoserine dehydrogenase

Gene

SPBC776.03

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 3 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable aspartokinase (SPBC19F5.04)
  2. Probable aspartate-semialdehyde dehydrogenase (SPCC1827.06c)
  3. Probable homoserine dehydrogenase (SPBC776.03)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable aspartokinase (SPBC19F5.04)
  2. Probable aspartate-semialdehyde dehydrogenase (SPCC1827.06c)
  3. Probable homoserine dehydrogenase (SPBC776.03)
  4. Probable homoserine kinase (SPBC4C3.03)
  5. Threonine synthase (thrc)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991NADPBy similarity
Binding sitei123 – 1231NADPBy similarity
Binding sitei216 – 2161SubstrateBy similarity
Active sitei231 – 2311Proton donorSequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 208NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis, Methionine biosynthesis, Threonine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00050; UER00063.
UPA00051; UER00465.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable homoserine dehydrogenase (EC:1.1.1.3)
Short name:
HDH
Gene namesi
ORF Names:SPBC776.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC776.03.
PomBaseiSPBC776.03.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Probable homoserine dehydrogenasePRO_0000066706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94671.
PRIDEiO94671.

PTM databases

iPTMnetiO94671.

Interactioni

Protein-protein interaction databases

BioGridi277663. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO94671.
SMRiO94671. Positions 4-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homoserine dehydrogenase family.Curated

Phylogenomic databases

HOGENOMiHOG000024037.
InParanoidiO94671.
KOiK00003.
OMAiVYHEATV.
OrthoDBiEOG7BGHWP.
PhylomeDBiO94671.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005106. Asp/hSer_DH_NAD-bd.
IPR001342. HDH_cat.
IPR019811. HDH_CS.
IPR022697. HDH_short.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF036497. HDH_short. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS01042. HOMOSER_DHGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASRTNVNV AIVGTGNIGG ELLNQIKGFN ENASTNGTTS FNVVAISSME
60 70 80 90 100
GHYVSKDYQP INLSEWKNLT SQNQGAYSLD ALVDFLAKSP LPAILVDNTA
110 120 130 140 150
SEAIAQSYPK FLSKKINIAT PNKKAFSASN DVYQNIIKAS KESGALLMHE
160 170 180 190 200
ASVGAGLPII STLKELIATG DEIIKIEGIF SGTLSYIFNV WSPNGKKGTA
210 220 230 240 250
SFSDIVKIAK QNGYTEPDPR DDLNGMDVAR KVTILSRIAG VHVESASSFP
260 270 280 290 300
VKSLIPEPLK SAVNAEEFLA GLPNFDSEFA SMREEAEKEG KVVRFVGEAD
310 320 330 340 350
VANKTTLVKL EKYDASHPFA NLQSSDNIIS FTTKRYHTRP LVVIGAGAGA
360 370
AVTAAGVLGD MIKIMSQVRA SDAPSA
Length:376
Mass (Da):40,037
Last modified:May 1, 1999 - v1
Checksum:i3A2BC3A2CBA9263C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22876.1.
PIRiT40673.
RefSeqiNP_596318.1. NM_001022240.2.

Genome annotation databases

EnsemblFungiiSPBC776.03.1; SPBC776.03.1:pep; SPBC776.03.
GeneIDi2541148.
KEGGispo:SPBC776.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22876.1.
PIRiT40673.
RefSeqiNP_596318.1. NM_001022240.2.

3D structure databases

ProteinModelPortaliO94671.
SMRiO94671. Positions 4-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277663. 1 interaction.

PTM databases

iPTMnetiO94671.

Proteomic databases

MaxQBiO94671.
PRIDEiO94671.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC776.03.1; SPBC776.03.1:pep; SPBC776.03.
GeneIDi2541148.
KEGGispo:SPBC776.03.

Organism-specific databases

EuPathDBiFungiDB:SPBC776.03.
PomBaseiSPBC776.03.

Phylogenomic databases

HOGENOMiHOG000024037.
InParanoidiO94671.
KOiK00003.
OMAiVYHEATV.
OrthoDBiEOG7BGHWP.
PhylomeDBiO94671.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00063.
UPA00051; UER00465.

Miscellaneous databases

NextBioi20802261.
PROiO94671.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005106. Asp/hSer_DH_NAD-bd.
IPR001342. HDH_cat.
IPR019811. HDH_CS.
IPR022697. HDH_short.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF036497. HDH_short. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS01042. HOMOSER_DHGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiDHOM_SCHPO
AccessioniPrimary (citable) accession number: O94671
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1999
Last modified: January 20, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.