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Protein

NAD-dependent histone deacetylase sir2

Gene

sir2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in silencing within the mating-type region, at the telomeres, and according to PubMed:12867036 also within centromeric DNA regions. Required for the localization of swi6 to the telomeres, silent mating type region, and according to PubMed:12867036 to the centromeric DNA regions. According to PubMed:15545655 not required for the localization of swi6 to centromeric foci. Deacetylates histone H3 on 'Lys-9' and 'Lys-16' of histone H4. This has a direct role in heterochromatin assembly.2 Publications

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei266 – 2661Proton acceptorPROSITE-ProRule annotation
Metal bindingi274 – 2741ZincPROSITE-ProRule annotation
Metal bindingi277 – 2771ZincPROSITE-ProRule annotation
Metal bindingi298 – 2981ZincPROSITE-ProRule annotation
Metal bindingi301 – 3011ZincPROSITE-ProRule annotation
Binding sitei416 – 4161NAD; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 18320NADBy similarityAdd
BLAST
Nucleotide bindingi246 – 2494NADBy similarity
Nucleotide bindingi373 – 3753NADBy similarity
Nucleotide bindingi398 – 4003NADBy similarity

GO - Molecular functioni

  • histone deacetylase activity Source: UniProtKB
  • histone deacetylase activity (H3-K14 specific) Source: PomBase
  • histone deacetylase activity (H3-K4 specific) Source: PomBase
  • histone deacetylase activity (H3-K9 specific) Source: PomBase
  • histone deacetylase activity (H4-K16 specific) Source: PomBase
  • NAD+ binding Source: PomBase
  • NAD-dependent histone deacetylase activity (H3-K9 specific) Source: PomBase
  • NAD-dependent histone deacetylase activity (H4-K16 specific) Source: PomBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin silencing Source: UniProtKB
  • chromatin silencing at centromere Source: PomBase
  • chromatin silencing at centromere outer repeat region Source: PomBase
  • chromatin silencing at silent mating-type cassette Source: PomBase
  • chromatin silencing at telomere Source: PomBase
  • DNA repair Source: UniProtKB-KW
  • histone deacetylation Source: PomBase
  • histone H3-K4 deacetylation Source: PomBase
  • histone H3-K9 deacetylation Source: PomBase
  • histone H4-K12 deacetylation Source: PomBase
  • histone H4-K16 deacetylation Source: PomBase
  • negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  • positive regulation of heterochromatin assembly Source: PomBase
  • protein localization to chromosome, telomeric region Source: PomBase
  • regulation of histone H3-K9 methylation Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Repressor

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent histone deacetylase sir2 (EC:3.5.1.-)
Alternative name(s):
Regulatory protein sir2
Silent information regulator 2
Gene namesi
Name:sir2
ORF Names:SPBC16D10.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16D10.07c.
PomBaseiSPBC16D10.07c. sir2.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric outer repeat region Source: GOC
  • chromosome, telomeric region Source: UniProtKB-SubCell
  • mating-type region heterochromatin Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear pericentric heterochromatin Source: PomBase
  • nuclear rDNA heterochromatin Source: PomBase
  • nuclear subtelomeric heterochromatin Source: PomBase
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475NAD-dependent histone deacetylase sir2PRO_0000110279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94640.

PTM databases

iPTMnetiO94640.
SwissPalmiO94640.

Interactioni

Protein-protein interaction databases

BioGridi280418. 48 interactions.
IntActiO94640. 1 interaction.
MINTiMINT-4685768.

Structurei

3D structure databases

ProteinModelPortaliO94640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 432286Deacetylase sirtuin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the sirtuin family. Class I subfamily.Curated
Contains 1 deacetylase sirtuin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000191845.
InParanoidiO94640.
KOiK11121.
OMAiHENEVST.
OrthoDBiEOG092C1NXT.
PhylomeDBiO94640.

Family and domain databases

Gene3Di3.30.1600.10. 3 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR007654. NAD-dep_histone_deAcase_SIR2_N.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF04574. DUF592. 1 hit.
PF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 2 hits.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94640-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNPLDNNM PTTPVEEKIP VASYSPSSSG SSSGASLLVD IMCGSKETED
60 70 80 90 100
EEVDSDEWDK PETENISDLD ERSEMVRYLR ASGYAKFLEK YLIEEELPVR
110 120 130 140 150
SILKKLGINL PSALEEFEDI DLLPLLKEVL KREVARRIKL PHFNTFEDVV
160 170 180 190 200
NLLKKAKNVV VLVGAGISTS LGILDFRSDN GFYARLARHG LSEPSEMFDI
210 220 230 240 250
HTFRENPEIF YTFARDLLPE TNHYSPSHAF IRLLEKKNKL STLFTQNIDN
260 270 280 290 300
LEKKTGLSDN KIIQCHGSFA TATCIKCKHK VDGSELYEDI RNQRVSYCNE
310 320 330 340 350
CGKPPLKLRR VGQNKKEKHY FSDGDSESSE DDLAQPGIMK PDITFFGEAL
360 370 380 390 400
PDSFFNKVGS GELEETDLLI CIGTSLKVAP VSELISVIPP TTPQIYISRT
410 420 430 440 450
PVRHTQFDVN FLSPYCDWVI VEICKRAGWL NELQALCDLP ECHSGSKTRA
460 470
FETDLDIKFE EPSTYHITST TNGSC
Length:475
Mass (Da):53,442
Last modified:January 4, 2005 - v2
Checksum:i2C078420B5CEE4F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAG47122.1.
PIRiT39571.
RefSeqiNP_001018840.1. NM_001022423.2.

Genome annotation databases

EnsemblFungiiSPBC16D10.07c.1; SPBC16D10.07c.1:pep; SPBC16D10.07c.
GeneIDi3361342.
KEGGispo:SPBC16D10.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAG47122.1.
PIRiT39571.
RefSeqiNP_001018840.1. NM_001022423.2.

3D structure databases

ProteinModelPortaliO94640.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280418. 48 interactions.
IntActiO94640. 1 interaction.
MINTiMINT-4685768.

PTM databases

iPTMnetiO94640.
SwissPalmiO94640.

Proteomic databases

MaxQBiO94640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC16D10.07c.1; SPBC16D10.07c.1:pep; SPBC16D10.07c.
GeneIDi3361342.
KEGGispo:SPBC16D10.07c.

Organism-specific databases

EuPathDBiFungiDB:SPBC16D10.07c.
PomBaseiSPBC16D10.07c. sir2.

Phylogenomic databases

HOGENOMiHOG000191845.
InParanoidiO94640.
KOiK11121.
OMAiHENEVST.
OrthoDBiEOG092C1NXT.
PhylomeDBiO94640.

Enzyme and pathway databases

ReactomeiR-SPO-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

PROiO94640.

Family and domain databases

Gene3Di3.30.1600.10. 3 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR007654. NAD-dep_histone_deAcase_SIR2_N.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF04574. DUF592. 1 hit.
PF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 2 hits.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIR2_SCHPO
AccessioniPrimary (citable) accession number: O94640
Secondary accession number(s): Q6H8I3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.