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Protein

NAD-dependent histone deacetylase sir2

Gene

sir2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in silencing within the mating-type region, at the telomeres, and according to PubMed:12867036 also within centromeric DNA regions. Required for the localization of swi6 to the telomeres, silent mating type region, and according to PubMed:12867036 to the centromeric DNA regions. According to PubMed:15545655 not required for the localization of swi6 to centromeric foci. Deacetylates histone H3 on 'Lys-9' and 'Lys-16' of histone H4. This has a direct role in heterochromatin assembly.2 Publications

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei266Proton acceptorPROSITE-ProRule annotation1
Metal bindingi274ZincPROSITE-ProRule annotation1
Metal bindingi277ZincPROSITE-ProRule annotation1
Metal bindingi298ZincPROSITE-ProRule annotation1
Metal bindingi301ZincPROSITE-ProRule annotation1
Binding sitei416NAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 183NADBy similarityAdd BLAST20
Nucleotide bindingi246 – 249NADBy similarity4
Nucleotide bindingi373 – 375NADBy similarity3
Nucleotide bindingi398 – 400NADBy similarity3

GO - Molecular functioni

  • histone deacetylase activity Source: UniProtKB
  • histone deacetylase activity (H3-K14 specific) Source: PomBase
  • histone deacetylase activity (H3-K4 specific) Source: PomBase
  • histone deacetylase activity (H3-K9 specific) Source: PomBase
  • histone deacetylase activity (H4-K16 specific) Source: PomBase
  • NAD+ binding Source: PomBase
  • NAD-dependent histone deacetylase activity (H3-K9 specific) Source: PomBase
  • NAD-dependent histone deacetylase activity (H4-K16 specific) Source: PomBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin silencing Source: UniProtKB
  • chromatin silencing at centromere Source: PomBase
  • chromatin silencing at centromere outer repeat region Source: PomBase
  • chromatin silencing at silent mating-type cassette Source: PomBase
  • chromatin silencing at telomere Source: PomBase
  • DNA repair Source: UniProtKB-KW
  • histone deacetylation Source: PomBase
  • histone H3-K4 deacetylation Source: PomBase
  • histone H3-K9 deacetylation Source: PomBase
  • histone H4-K12 deacetylation Source: PomBase
  • histone H4-K16 deacetylation Source: PomBase
  • negative regulation of transcription by RNA polymerase II Source: PomBase
  • positive regulation of heterochromatin assembly Source: PomBase
  • protein localization to chromosome, telomeric region Source: PomBase
  • regulation of histone H3-K9 methylation Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, Hydrolase, Repressor
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-3371453 Regulation of HSF1-mediated heat shock response
R-SPO-427359 SIRT1 negatively regulates rRNA expression

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent histone deacetylase sir2 (EC:3.5.1.-)
Alternative name(s):
Regulatory protein sir2
Silent information regulator 2
Gene namesi
Name:sir2
ORF Names:SPBC16D10.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16D10.07c
PomBaseiSPBC16D10.07c sir2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001102791 – 475NAD-dependent histone deacetylase sir2Add BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94640
PaxDbiO94640
PRIDEiO94640

PTM databases

iPTMnetiO94640
SwissPalmiO94640

Interactioni

Protein-protein interaction databases

BioGridi280418, 50 interactors
IntActiO94640, 1 interactor
STRINGi4896.SPBC16D10.07c.1

Structurei

3D structure databases

ProteinModelPortaliO94640
SMRiO94640
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini147 – 432Deacetylase sirtuin-typePROSITE-ProRule annotationAdd BLAST286

Sequence similaritiesi

Belongs to the sirtuin family. Class I subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000191845
InParanoidiO94640
KOiK11121
OMAiTNYSQNI
OrthoDBiEOG092C1NXT
PhylomeDBiO94640

Family and domain databases

Gene3Di3.30.1600.10, 2 hits
InterProiView protein in InterPro
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR007654 NAD-dep_histone_deAcase_SIR2_N
IPR003000 Sirtuin
IPR026591 Sirtuin_cat_small_dom_sf
IPR026590 Ssirtuin_cat_dom
PfamiView protein in Pfam
PF04574 DUF592, 1 hit
PF02146 SIR2, 1 hit
SUPFAMiSSF52467 SSF52467, 2 hits
PROSITEiView protein in PROSITE
PS50305 SIRTUIN, 1 hit

Sequencei

Sequence statusi: Complete.

O94640-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNPLDNNM PTTPVEEKIP VASYSPSSSG SSSGASLLVD IMCGSKETED
60 70 80 90 100
EEVDSDEWDK PETENISDLD ERSEMVRYLR ASGYAKFLEK YLIEEELPVR
110 120 130 140 150
SILKKLGINL PSALEEFEDI DLLPLLKEVL KREVARRIKL PHFNTFEDVV
160 170 180 190 200
NLLKKAKNVV VLVGAGISTS LGILDFRSDN GFYARLARHG LSEPSEMFDI
210 220 230 240 250
HTFRENPEIF YTFARDLLPE TNHYSPSHAF IRLLEKKNKL STLFTQNIDN
260 270 280 290 300
LEKKTGLSDN KIIQCHGSFA TATCIKCKHK VDGSELYEDI RNQRVSYCNE
310 320 330 340 350
CGKPPLKLRR VGQNKKEKHY FSDGDSESSE DDLAQPGIMK PDITFFGEAL
360 370 380 390 400
PDSFFNKVGS GELEETDLLI CIGTSLKVAP VSELISVIPP TTPQIYISRT
410 420 430 440 450
PVRHTQFDVN FLSPYCDWVI VEICKRAGWL NELQALCDLP ECHSGSKTRA
460 470
FETDLDIKFE EPSTYHITST TNGSC
Length:475
Mass (Da):53,442
Last modified:January 4, 2005 - v2
Checksum:i2C078420B5CEE4F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAG47122.1
PIRiT39571
RefSeqiNP_001018840.1, NM_001022423.2

Genome annotation databases

EnsemblFungiiSPBC16D10.07c.1; SPBC16D10.07c.1:pep; SPBC16D10.07c
GeneIDi3361342
KEGGispo:SPBC16D10.07c

Similar proteinsi

Entry informationi

Entry nameiSIR2_SCHPO
AccessioniPrimary (citable) accession number: O94640
Secondary accession number(s): Q6H8I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 4, 2005
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health