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Protein

Ergothioneine biosynthesis protein 1

Gene

egt1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the SAM-dependent triple methylation of the alpha-amino group of histidine to form hercynine and subsequent conjugation with cysteine and oxygen to form hercynylcysteine sulfoxide, the first two steps in the biosynthesis pathway of ergothioneine (PubMed:24828577). May play a role in meiosis (PubMed:16303567).2 Publications

Catalytic activityi

3 S-adenosyl-L-methionine + L-histidine = 3 S-adenosyl-L-homocysteine + hercynine.By similarity
Hercynine + L-cysteine + O2 = S-hercyn-2-yl-L-cysteine S-oxide + H2O.By similarity

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per monomer.By similarity

Pathwayi: ergothioneine biosynthesis

This protein is involved in the pathway ergothioneine biosynthesis, which is part of Amino-acid biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway ergothioneine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51L-histidineBy similarity1
Binding sitei85S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei91S-adenosyl-L-methionineBy similarity1
Binding sitei112S-adenosyl-L-methionineBy similarity1
Binding sitei172L-histidineBy similarity1
Binding sitei212L-histidineBy similarity1
Metal bindingi382Iron; via tele nitrogenBy similarity1
Metal bindingi476Iron; via tele nitrogenBy similarity1
Metal bindingi480Iron; via tele nitrogenBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: PomBase
  • dimethylhistidine N-methyltransferase activity Source: UniProtKB-EC
  • hercynylcysteine sulfoxide synthase Source: PomBase
  • hercynylselenocysteine synthase Source: PomBase
  • histidine N-methyltransferase activity Source: PomBase
  • monooxygenase activity Source: UniProtKB-KW

GO - Biological processi

  • ergothioneine biosynthetic process Source: PomBase
  • hercynylcysteine sulfoxide biosynthetic process Source: PomBase
  • hercynylselenocysteine biosynthetic process Source: PomBase
  • meiotic cell cycle Source: UniProtKB-KW
  • N-alpha,N-alpha,N-alpha-trimethyl-L-histidine biosynthesis from histidine Source: PomBase
  • selenoneine biosynthetic process Source: PomBase

Keywordsi

Molecular functionMethyltransferase, Monooxygenase, Oxidoreductase, Transferase
Biological processMeiosis
LigandIron, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA01014

Names & Taxonomyi

Protein namesi
Recommended name:
Ergothioneine biosynthesis protein 11 Publication
Alternative name(s):
Meiotically up-regulated gene 158 protein1 Publication
Including the following 2 domains:
L-histidine N(alpha)-methyltransferaseBy similarity (EC:2.1.1.44By similarity)
Hercynylcysteine S-oxide synthaseBy similarity (EC:1.14.99.51By similarity)
Gene namesi
Name:egt11 Publication
Synonyms:mug1581 Publication
ORF Names:SPBC1604.01Imported, SPBC1677.01c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1604.01
PomBaseiSPBC1604.01 egt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Completely lacks ergothioneine and its precursors (trimethyl histidine/hercynine and hercynylcysteine sulfoxide).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002785111 – 773Ergothioneine biosynthesis protein 1Add BLAST773

Proteomic databases

MaxQBiO94632
PaxDbiO94632
PRIDEiO94632

Interactioni

Protein-protein interaction databases

STRINGi4896.SPBC1604.01.1

Structurei

3D structure databases

ProteinModelPortaliO94632
SMRiO94632
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 322L-histidine N(alpha)-methyltransferaseCuratedAdd BLAST307
Regioni142 – 143S-adenosyl-L-methionine bindingBy similarity2
Regioni287 – 289L-histidine bindingBy similarity3
Regioni347 – 772Hercynylcysteine S-oxide synthaseCuratedAdd BLAST426

Sequence similaritiesi

In the N-terminal section; belongs to the methyltransferase superfamily. EgtD family.Curated
In the C-terminal section; belongs to the EgtB family.Curated

Phylogenomic databases

InParanoidiO94632
KOiK20246
OMAiITNGEYA
OrthoDBiEOG092C3SV3
PhylomeDBiO94632

Family and domain databases

InterProiView protein in InterPro
IPR016187 CTDL_fold
IPR019257 MeTrfase_dom
IPR029063 SAM-dependent_MTases
IPR017805 SAM_MeTrfase_EsaF-type_put
IPR005532 SUMF_dom
PfamiView protein in Pfam
PF03781 FGE-sulfatase, 1 hit
PF10017 Methyltransf_33, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
SSF56436 SSF56436, 1 hit
TIGRFAMsiTIGR03439 methyl_EasF, 1 hit

Sequencei

Sequence statusi: Complete.

O94632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEIENIGAL EVLFSPESIE QSLKRCQLPS TLLYDEKGLR LFDEITNLKE
60 70 80 90 100
YYLYESELDI LKKFSDSIAN QLLSPDLPNT VIELGCGNMR KTKLLLDAFE
110 120 130 140 150
KKGCDVHFYA LDLNEAELQK GLQELRQTTN YQHVKVSGIC GCFERLLQCL
160 170 180 190 200
DRFRSEPNSR ISMLYLGASI GNFDRKSAAS FLRSFASRLN IHDNLLISFD
210 220 230 240 250
HRNKAELVQL AYDDPYRITE KFEKNILASV NAVFGENLFD ENDWEYKSVY
260 270 280 290 300
DEDLGVHRAY LQAKNEVTVI KGPMFFQFKP SHLILIEESW KNSDQECRQI
310 320 330 340 350
IEKGDFKLVS KYESTIADYS TYVITKQFPA MLQLPLQPCP SLAEWDALRK
360 370 380 390 400
VWLFITNKLL NKDNMYTAWI PLRHPPIFYI GHVPVFNDIY LTKIVKNKAT
410 420 430 440 450
ANKKHFWEWF QRGIDPDIED PSKCHWHSEV PESWPSPDQL REYEKESWEY
460 470 480 490 500
HIVKLCKAMD ELSTSEKRIL WLCYEHVAMH VETTLYIYVQ SFQNANQTVS
510 520 530 540 550
ICGSLPEPAE KLTKAPLWVN VPETEIAVGM PLTTQYTSVG SNLQSSDLSA
560 570 580 590 600
HENTDELFYF AWDNEKPMRK KLVSSFSIAN RPISNGEYLD FINKKSKTER
610 620 630 640 650
VYPKQWAEID GTLYIRTMYG LLPLDDYLGW PVMTSYDDLN NYASSQGCRL
660 670 680 690 700
PTEDELNCFY DRVLERTDEP YVSTEGKATG FQQLHPLALS DNSSNQIFTG
710 720 730 740 750
AWEWTSTVLE KHEDFEPEEL YPDYTRDFFD GKHNVVLGGS FATATRISNR
760 770
RSFRNFYQAG YKYAWIGARL VKN
Length:773
Mass (Da):89,714
Last modified:February 20, 2007 - v4
Checksum:i4E9EF9AC7A4D89C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA22334.1
PIRiT39513
RefSeqiNP_596639.2, NM_001022560.3

Genome annotation databases

EnsemblFungiiSPBC1604.01.1; SPBC1604.01.1:pep; SPBC1604.01
GeneIDi2539656
KEGGispo:SPBC1604.01

Similar proteinsi

Entry informationi

Entry nameiEGT1_SCHPO
AccessioniPrimary (citable) accession number: O94632
Secondary accession number(s): O94367, Q1MTP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: May 23, 2018
This is version 102 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

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