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Protein

DNA repair protein rev1

Gene

rev1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei240 – 2401dCTPBy similarity
Metal bindingi283 – 2831Magnesium 1PROSITE-ProRule annotation
Metal bindingi283 – 2831Magnesium 2PROSITE-ProRule annotation
Metal bindingi284 – 2841Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei329 – 3291dCTPBy similarity
Metal bindingi378 – 3781Magnesium 1PROSITE-ProRule annotation
Binding sitei378 – 3781dCTPBy similarity
Metal bindingi379 – 3791Magnesium 1PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2875dCTP bindingBy similarity
Nucleotide bindingi317 – 3237dCTP bindingBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein rev1By similarity (EC:2.7.7.-)
Alternative name(s):
Reversionless protein 1By similarity
Gene namesi
Name:rev1Imported
ORF Names:SPBC1347.01c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1347.01c.
PomBaseiSPBC1347.01c. rev1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: PomBase
  • mitotic spindle Source: PomBase
  • nucleolus Source: PomBase
  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935DNA repair protein rev1PRO_0000361684Add
BLAST

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei578 – 5781Interaction with target DNABy similarity
Sitei589 – 5891Interaction with target DNABy similarity
Sitei591 – 5911Interaction with target DNABy similarity

Protein-protein interaction databases

BioGridi276276. 5 interactions.
IntActiO94623. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO94623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 14789BRCTPROSITE-ProRule annotationAdd
BLAST
Domaini279 – 460182UmuCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni235 – 24511Interaction with target DNABy similarityAdd
BLAST
Regioni310 – 3123Interaction with target DNABy similarity
Regioni460 – 4634Interaction with target DNABy similarity
Regioni517 – 5259Interaction with target DNABy similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Sequence analysis
Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiO94623.
KOiK03515.
OMAiMKEADLP.
OrthoDBiEOG783N3S.
PhylomeDBiO94623.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR031991. Rev1_C.
IPR001126. UmuC.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF16727. REV1_C. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFNQRKRRR PVGIADFDEA NDEAYESVGF HDYADYFSRK QRKLQNQNAA
60 70 80 90 100
LYKSIDEDSK SDLFHGLAIA INGYTKPSYT ELRQMIVSNG GTFIQYVDGK
110 120 130 140 150
TSISYLVCSF LTPSKARQWK HQKVVKPEWI VDCIKQKKIL PWINYRTFQA
160 170 180 190 200
SSAQATLSFV ASKPSQPEGN LEDIQTSSQE EEHDNEKDKT KESKAKGFLD
210 220 230 240 250
DLSGLSASSL HNYQLLKNPN VRNSTTQNQD FLENFFSSSR LHHLSTWKAD
260 270 280 290 300
FKNEIQAMTT ASEPVRPIMK DKSKKSRFLL HVDFDCFFAS VSTRFSHELR
310 320 330 340 350
LKPVAVAHGI KNSEIASCNY EARKFGIKNG MYVGTAKNLC PSLRVVDYDF
360 370 380 390 400
GAYESVSREF YTILVNTLHD YIKVISIDEA LLDITSSVSS FQDCFEIAES
410 420 430 440 450
IRSQVREKTN CEVSVGIGPN VLLARLALRK AKPHNVYSLS IENVFDVLSP
460 470 480 490 500
LSVQDLPGVG SSQAQKLFNL YGVRTIGQLQ RIEKFNLQET FGVNYGLHLY
510 520 530 540 550
NISRGIDTDI INNETPRRSI SVDVNWGVRF VFQEDGIDFL KRLLHELLSR
560 570 580 590 600
MGKCQVLLHQ IQLRILKRAD GAPFSPPKYL GAGEVTSFTK SSTFTSATNS
610 620 630 640 650
FDLIWKKVTS MYKTINVDPG DVRGIGLQAL KIIKDNSKIR KDYRSIQSIT
660 670 680 690 700
SRNKVSLKGA SVDISSKDKE IISQKKQLSP KLIPSTPYDL PSSSQISSSA
710 720 730 740 750
LAQLPPSMQS DIQQQLRLQK RSITEYPSQL DPLFMVELPT PIRNEVNDNH
760 770 780 790 800
EIAMNKRLSL KSHADNKIDE RGKKKIRQEN AFDKLLQISK KSKTINKPNV
810 820 830 840 850
DYLTLKELPK DLQKQILKES NLQKSDLISE VKLEKPHIVT FQHVQSLEDL
860 870 880 890 900
RGLLTKWYSK ASKGPNIHDV NYFANYVCRV IREEKNLGKA QMMLKWLYQL
910 920 930
NRKECNKPWE KAIDKIIETV QGECLQRNIP PLMIF
Length:935
Mass (Da):106,526
Last modified:August 21, 2007 - v3
Checksum:iB1A472DDA37085FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22130.2.
RefSeqiNP_596693.2. NM_001022616.3.

Genome annotation databases

EnsemblFungiiSPBC1347.01c.1; SPBC1347.01c.1:pep; SPBC1347.01c.
GeneIDi2539723.
KEGGispo:SPBC1347.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22130.2.
RefSeqiNP_596693.2. NM_001022616.3.

3D structure databases

ProteinModelPortaliO94623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276276. 5 interactions.
IntActiO94623. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC1347.01c.1; SPBC1347.01c.1:pep; SPBC1347.01c.
GeneIDi2539723.
KEGGispo:SPBC1347.01c.

Organism-specific databases

EuPathDBiFungiDB:SPBC1347.01c.
PomBaseiSPBC1347.01c. rev1.

Phylogenomic databases

InParanoidiO94623.
KOiK03515.
OMAiMKEADLP.
OrthoDBiEOG783N3S.
PhylomeDBiO94623.

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.

Miscellaneous databases

PROiO94623.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR031991. Rev1_C.
IPR001126. UmuC.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF16727. REV1_C. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiREV1_SCHPO
AccessioniPrimary (citable) accession number: O94623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: August 21, 2007
Last modified: June 8, 2016
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.