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Protein

DNA repair protein rev1

Gene

rev1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei240dCTPBy similarity1
Metal bindingi283Magnesium 1PROSITE-ProRule annotation1
Metal bindingi283Magnesium 2PROSITE-ProRule annotation1
Metal bindingi284Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei329dCTPBy similarity1
Metal bindingi378Magnesium 1PROSITE-ProRule annotation1
Binding sitei378dCTPBy similarity1
Metal bindingi379Magnesium 1PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 287dCTP bindingBy similarity5
Nucleotide bindingi317 – 323dCTP bindingBy similarity7

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: PomBase
  • error-free translesion synthesis Source: PomBase
  • error-prone translesion synthesis Source: PomBase
  • mitochondrial DNA repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein rev1By similarity (EC:2.7.7.-)
Alternative name(s):
Reversionless protein 1By similarity
Gene namesi
Name:rev1Imported
ORF Names:SPBC1347.01c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1347.01c.
PomBaseiSPBC1347.01c. rev1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: PomBase
  • mitotic spindle Source: PomBase
  • nucleolus Source: PomBase
  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003616841 – 935DNA repair protein rev1Add BLAST935

Proteomic databases

PRIDEiO94623.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei578Interaction with target DNABy similarity1
Sitei589Interaction with target DNABy similarity1
Sitei591Interaction with target DNABy similarity1

Protein-protein interaction databases

BioGridi276276. 5 interactors.
IntActiO94623. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliO94623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 147BRCTPROSITE-ProRule annotationAdd BLAST89
Domaini279 – 460UmuCPROSITE-ProRule annotationAdd BLAST182

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 245Interaction with target DNABy similarityAdd BLAST11
Regioni310 – 312Interaction with target DNABy similarity3
Regioni460 – 463Interaction with target DNABy similarity4
Regioni517 – 525Interaction with target DNABy similarity9

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Sequence analysis
Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiO94623.
KOiK03515.
OMAiEIKRIMM.
OrthoDBiEOG092C08ZW.
PhylomeDBiO94623.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR031991. Rev1_C.
IPR001126. UmuC.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF16727. REV1_C. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFNQRKRRR PVGIADFDEA NDEAYESVGF HDYADYFSRK QRKLQNQNAA
60 70 80 90 100
LYKSIDEDSK SDLFHGLAIA INGYTKPSYT ELRQMIVSNG GTFIQYVDGK
110 120 130 140 150
TSISYLVCSF LTPSKARQWK HQKVVKPEWI VDCIKQKKIL PWINYRTFQA
160 170 180 190 200
SSAQATLSFV ASKPSQPEGN LEDIQTSSQE EEHDNEKDKT KESKAKGFLD
210 220 230 240 250
DLSGLSASSL HNYQLLKNPN VRNSTTQNQD FLENFFSSSR LHHLSTWKAD
260 270 280 290 300
FKNEIQAMTT ASEPVRPIMK DKSKKSRFLL HVDFDCFFAS VSTRFSHELR
310 320 330 340 350
LKPVAVAHGI KNSEIASCNY EARKFGIKNG MYVGTAKNLC PSLRVVDYDF
360 370 380 390 400
GAYESVSREF YTILVNTLHD YIKVISIDEA LLDITSSVSS FQDCFEIAES
410 420 430 440 450
IRSQVREKTN CEVSVGIGPN VLLARLALRK AKPHNVYSLS IENVFDVLSP
460 470 480 490 500
LSVQDLPGVG SSQAQKLFNL YGVRTIGQLQ RIEKFNLQET FGVNYGLHLY
510 520 530 540 550
NISRGIDTDI INNETPRRSI SVDVNWGVRF VFQEDGIDFL KRLLHELLSR
560 570 580 590 600
MGKCQVLLHQ IQLRILKRAD GAPFSPPKYL GAGEVTSFTK SSTFTSATNS
610 620 630 640 650
FDLIWKKVTS MYKTINVDPG DVRGIGLQAL KIIKDNSKIR KDYRSIQSIT
660 670 680 690 700
SRNKVSLKGA SVDISSKDKE IISQKKQLSP KLIPSTPYDL PSSSQISSSA
710 720 730 740 750
LAQLPPSMQS DIQQQLRLQK RSITEYPSQL DPLFMVELPT PIRNEVNDNH
760 770 780 790 800
EIAMNKRLSL KSHADNKIDE RGKKKIRQEN AFDKLLQISK KSKTINKPNV
810 820 830 840 850
DYLTLKELPK DLQKQILKES NLQKSDLISE VKLEKPHIVT FQHVQSLEDL
860 870 880 890 900
RGLLTKWYSK ASKGPNIHDV NYFANYVCRV IREEKNLGKA QMMLKWLYQL
910 920 930
NRKECNKPWE KAIDKIIETV QGECLQRNIP PLMIF
Length:935
Mass (Da):106,526
Last modified:August 21, 2007 - v3
Checksum:iB1A472DDA37085FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22130.2.
RefSeqiNP_596693.2. NM_001022616.3.

Genome annotation databases

EnsemblFungiiSPBC1347.01c.1; SPBC1347.01c.1:pep; SPBC1347.01c.
GeneIDi2539723.
KEGGispo:SPBC1347.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22130.2.
RefSeqiNP_596693.2. NM_001022616.3.

3D structure databases

ProteinModelPortaliO94623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276276. 5 interactors.
IntActiO94623. 1 interactor.

Proteomic databases

PRIDEiO94623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC1347.01c.1; SPBC1347.01c.1:pep; SPBC1347.01c.
GeneIDi2539723.
KEGGispo:SPBC1347.01c.

Organism-specific databases

EuPathDBiFungiDB:SPBC1347.01c.
PomBaseiSPBC1347.01c. rev1.

Phylogenomic databases

InParanoidiO94623.
KOiK03515.
OMAiEIKRIMM.
OrthoDBiEOG092C08ZW.
PhylomeDBiO94623.

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.

Miscellaneous databases

PROiO94623.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR031991. Rev1_C.
IPR001126. UmuC.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF16727. REV1_C. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiREV1_SCHPO
AccessioniPrimary (citable) accession number: O94623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: August 21, 2007
Last modified: November 30, 2016
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.