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O94609 (UBA1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-activating enzyme E1 1
Alternative name(s):
Poly(A)+ RNA transport protein 3
Gene names
Name:ptr3
ORF Names:SPBC1604.21c, SPBC211.09
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›

Protein attributes

Sequence length1012 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm. Nucleus.

Sequence similarities

Belongs to the ubiquitin-activating E1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10121012Ubiquitin-activating enzyme E1 1
PRO_0000194976

Regions

Nucleotide binding437 – 46630ATP By similarity

Sites

Active site5931Glycyl thioester intermediate By similarity

Secondary structure

1 ............................................................................................................................................................................ 1012
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O94609 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: AB5207808ACC6C2D

FASTA1,012112,949
        10         20         30         40         50         60 
MSNNMNIDQT DQNTIDEGLY SRQLYVLGHE AMKQMSQSNV LIIGCKGLGV EIAKNVCLAG 

        70         80         90        100        110        120 
VKSVTLYDPQ PTRIEDLSSQ YFLTEDDIGV PRAKVTVSKL AELNQYVPVS VVDELSTEYL 

       130        140        150        160        170        180 
KNFKCVVVTE TSLTKQLEIN DFTHKNHIAY IAADSRGLFG SIFCDFGENF ICTDTDGNEP 

       190        200        210        220        230        240 
LTGMIASITD DGVVTMLEET RHGLENGDFV KFTEVKGMPG LNDGTPRKVE VKGPYTFSIG 

       250        260        270        280        290        300 
SVKDLGSAGY NGVFTQVKVP TKISFKSLRE SLKDPEYVYP DFGKMMRPPQ YHIAFQALSA 

       310        320        330        340        350        360 
FADAHEGSLP RPRNDIDAAE FFEFCKKIAS TLQFDVELDE KLIKEISYQA RGDLVAMSAF 

       370        380        390        400        410        420 
LGGAVAQEVL KATTSKFYPL KQYFYFDSLE SLPSSVTISE ETCKPRGCRY DGQIAVFGSE 

       430        440        450        460        470        480 
FQEKIASLST FLVGAGAIGC EMLKNWAMMG VATGESGHIS VTDMDSIEKS NLNRQFLFRP 

       490        500        510        520        530        540 
RDVGKLKSEC ASTAVSIMNP SLTGKITSYQ ERVGPESEGI FGDEFFEKLS LVTNALDNVE 

       550        560        570        580        590        600 
ARMYVDRRCV FFEKPLLESG TLGTKGNTQV VVPHLTESYG SSQDPPEKSF PICTLKNFPN 

       610        620        630        640        650        660 
RIEHTIAWAR DLFEGLFKQP IDNVNMYLSS PNFLETSLKT SSNPREVLEN IRDYLVTEKP 

       670        680        690        700        710        720 
LSFEECIMWA RLQFDKFFNN NIQQLLFNFP KDSVTSTGQP FWSGPKRAPT PLSFDIHNRE 

       730        740        750        760        770        780 
HFDFIVAAAS LYAFNYGLKS ETDPAIYERV LAGYNPPPFA PKSGIKIQVN ENEEAPETAA 

       790        800        810        820        830        840 
NKDKQELKSI ADSLPPPSSL VGFRLTPAEF EKDDDSNHHI DFITAASNLR AMNYDITPAD 

       850        860        870        880        890        900 
RFKTKFVAGK IVPAMCTSTA VVSGLVCLEL VKLVDGKKKI EEYKNGFFNL AIGLFTFSDP 

       910        920        930        940        950        960 
IASPKMKVNG KEIDKIWDRY NLPDCTLQEL IDYFQKEEGL EVTMLSSGVS LLYANFQPPK 

       970        980        990       1000       1010 
KLAERLPLKI SELVEQITKK KLEPFRKHLV LEICCDDANG EDVEVPFICI KL

« Hide

References

« Hide 'large scale' references
[1]"Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe."
Azad A.K., Tani T., Shiki N., Tsuneyoshi S., Urushiyama S., Ohshima Y.
Mol. Biol. Cell 8:825-841(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D87259 Genomic DNA. Translation: BAA75198.1.
CU329671 Genomic DNA. Translation: CAA22354.1.
PIRT50344.
T52000.
RefSeqXP_001713148.1. XM_001713096.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4II2X-ray2.20A13-1012[»]
4II3X-ray2.90A/C13-1012[»]
ProteinModelPortalO94609.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48686N.
STRING4896.SPBC1604.21c-1.

Proteomic databases

PaxDbO94609.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC1604.21c.1; SPBC1604.21c.1:pep; SPBC1604.21c.
GeneID2540744.
KEGGspo:SPBC1604.21c.

Organism-specific databases

PomBaseSPBC1604.21c.

Phylogenomic databases

eggNOGCOG0476.
HOGENOMHOG000167329.
KOK03178.
OMAFESGDYV.
OrthoDBEOG4NKG3P.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D1.10.3240.10. 2 hits.
3.40.50.720. 4 hits.
InterProIPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamPF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view]
PRINTSPR01849. UBIQUITINACT.
SMARTSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMSSF69572. MoeB. 2 hits.
TIGRFAMsTIGR01408. Ube1. 1 hit.
PROSITEPS00536. UBIQUITIN_ACTIVAT_1. False negative.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801866.

Entry information

Entry nameUBA1_SCHPO
AccessionPrimary (citable) accession number: O94609
Secondary accession number(s): Q9P7R2, Q9USY9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents