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O94609

- UBA1_SCHPO

UniProt

O94609 - UBA1_SCHPO

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Protein

Ubiquitin-activating enzyme E1 1

Gene
ptr3, SPBC1604.21c, SPBC211.09
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei593 – 5931Glycyl thioester intermediate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi437 – 46630ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: PomBase
  2. magnesium ion binding Source: PomBase
  3. protein binding Source: PomBase
  4. ubiquitin activating enzyme activity Source: PomBase

GO - Biological processi

  1. mRNA export from nucleus Source: PomBase
  2. protein ubiquitination Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-activating enzyme E1 1
Alternative name(s):
Poly(A)+ RNA transport protein 3
Gene namesi
Name:ptr3
ORF Names:SPBC1604.21c, SPBC211.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC1604.21c.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10121012Ubiquitin-activating enzyme E1 1PRO_0000194976Add
BLAST

Proteomic databases

MaxQBiO94609.
PaxDbiO94609.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

BioGridi277267. 1 interaction.
DIPiDIP-48686N.
MINTiMINT-4685507.
STRINGi4896.SPBC1604.21c-1.

Structurei

Secondary structure

1
1012
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 2711
Helixi29 – 368
Beta strandi39 – 435
Helixi47 – 5913
Beta strandi62 – 676
Helixi74 – 785
Helixi85 – 873
Helixi92 – 10110
Beta strandi109 – 1113
Helixi118 – 1225
Beta strandi124 – 1285
Helixi133 – 14513
Beta strandi149 – 1568
Beta strandi159 – 1657
Beta strandi170 – 1767
Beta strandi182 – 1898
Beta strandi193 – 1975
Beta strandi209 – 2146
Helixi221 – 2233
Beta strandi233 – 2386
Beta strandi247 – 2515
Beta strandi253 – 2575
Beta strandi261 – 2633
Helixi268 – 2714
Beta strandi272 – 2743
Helixi282 – 2843
Helixi287 – 30418
Turni305 – 3073
Helixi315 – 33117
Helixi340 – 3489
Turni349 – 3513
Helixi355 – 37218
Beta strandi382 – 3876
Helixi389 – 3913
Beta strandi394 – 3963
Helixi400 – 4034
Helixi411 – 4177
Helixi419 – 4268
Beta strandi429 – 4335
Helixi437 – 44913
Turni450 – 4523
Beta strandi454 – 4563
Beta strandi458 – 4625
Helixi469 – 4713
Turni472 – 4743
Helixi480 – 4823
Helixi487 – 49812
Helixi500 – 5023
Beta strandi505 – 5095
Helixi515 – 5173
Turni518 – 5203
Helixi523 – 5275
Beta strandi530 – 5345
Helixi539 – 55214
Beta strandi556 – 5627
Beta strandi565 – 5717
Turni573 – 5753
Helixi579 – 5813
Helixi592 – 5976
Helixi602 – 61716
Helixi619 – 6279
Helixi633 – 6375
Turni638 – 6403
Helixi644 – 65512
Turni656 – 6583
Helixi663 – 67816
Helixi680 – 6889
Beta strandi698 – 7025
Helixi719 – 73517
Helixi744 – 7529
Helixi788 – 7925
Helixi797 – 7993
Helixi819 – 83315
Helixi841 – 8488
Helixi856 – 87419
Helixi880 – 8823
Beta strandi885 – 8895
Helixi890 – 8923
Beta strandi894 – 8985
Beta strandi905 – 9084
Beta strandi911 – 9144
Turni915 – 9173
Beta strandi919 – 9246
Helixi927 – 93610
Beta strandi941 – 9477
Beta strandi950 – 9545
Helixi959 – 9635
Turni964 – 9674
Helixi970 – 9778
Beta strandi988 – 9969
Beta strandi1007 – 10115

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4II2X-ray2.20A13-1012[»]
4II3X-ray2.90A/C13-1012[»]
ProteinModelPortaliO94609.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0476.
HOGENOMiHOG000167329.
KOiK03178.
OMAiKFHPVKQ.
OrthoDBiEOG7FZ07P.
PhylomeDBiO94609.

Family and domain databases

Gene3Di1.10.3240.10. 2 hits.
3.40.50.720. 4 hits.
InterProiIPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SMARTiSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01408. Ube1. 1 hit.
PROSITEiPS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94609-1 [UniParc]FASTAAdd to Basket

« Hide

MSNNMNIDQT DQNTIDEGLY SRQLYVLGHE AMKQMSQSNV LIIGCKGLGV     50
EIAKNVCLAG VKSVTLYDPQ PTRIEDLSSQ YFLTEDDIGV PRAKVTVSKL 100
AELNQYVPVS VVDELSTEYL KNFKCVVVTE TSLTKQLEIN DFTHKNHIAY 150
IAADSRGLFG SIFCDFGENF ICTDTDGNEP LTGMIASITD DGVVTMLEET 200
RHGLENGDFV KFTEVKGMPG LNDGTPRKVE VKGPYTFSIG SVKDLGSAGY 250
NGVFTQVKVP TKISFKSLRE SLKDPEYVYP DFGKMMRPPQ YHIAFQALSA 300
FADAHEGSLP RPRNDIDAAE FFEFCKKIAS TLQFDVELDE KLIKEISYQA 350
RGDLVAMSAF LGGAVAQEVL KATTSKFYPL KQYFYFDSLE SLPSSVTISE 400
ETCKPRGCRY DGQIAVFGSE FQEKIASLST FLVGAGAIGC EMLKNWAMMG 450
VATGESGHIS VTDMDSIEKS NLNRQFLFRP RDVGKLKSEC ASTAVSIMNP 500
SLTGKITSYQ ERVGPESEGI FGDEFFEKLS LVTNALDNVE ARMYVDRRCV 550
FFEKPLLESG TLGTKGNTQV VVPHLTESYG SSQDPPEKSF PICTLKNFPN 600
RIEHTIAWAR DLFEGLFKQP IDNVNMYLSS PNFLETSLKT SSNPREVLEN 650
IRDYLVTEKP LSFEECIMWA RLQFDKFFNN NIQQLLFNFP KDSVTSTGQP 700
FWSGPKRAPT PLSFDIHNRE HFDFIVAAAS LYAFNYGLKS ETDPAIYERV 750
LAGYNPPPFA PKSGIKIQVN ENEEAPETAA NKDKQELKSI ADSLPPPSSL 800
VGFRLTPAEF EKDDDSNHHI DFITAASNLR AMNYDITPAD RFKTKFVAGK 850
IVPAMCTSTA VVSGLVCLEL VKLVDGKKKI EEYKNGFFNL AIGLFTFSDP 900
IASPKMKVNG KEIDKIWDRY NLPDCTLQEL IDYFQKEEGL EVTMLSSGVS 950
LLYANFQPPK KLAERLPLKI SELVEQITKK KLEPFRKHLV LEICCDDANG 1000
EDVEVPFICI KL 1012
Length:1,012
Mass (Da):112,949
Last modified:May 1, 1999 - v1
Checksum:iAB5207808ACC6C2D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87259 Genomic DNA. Translation: BAA75198.1.
CU329671 Genomic DNA. Translation: CAA22354.1.
PIRiT50344.
T52000.
RefSeqiXP_001713148.1. XM_001713096.2.

Genome annotation databases

EnsemblFungiiSPBC1604.21c.1; SPBC1604.21c.1:pep; SPBC1604.21c.
GeneIDi2540744.
KEGGispo:SPBC1604.21c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87259 Genomic DNA. Translation: BAA75198.1 .
CU329671 Genomic DNA. Translation: CAA22354.1 .
PIRi T50344.
T52000.
RefSeqi XP_001713148.1. XM_001713096.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4II2 X-ray 2.20 A 13-1012 [» ]
4II3 X-ray 2.90 A/C 13-1012 [» ]
ProteinModelPortali O94609.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277267. 1 interaction.
DIPi DIP-48686N.
MINTi MINT-4685507.
STRINGi 4896.SPBC1604.21c-1.

Proteomic databases

MaxQBi O94609.
PaxDbi O94609.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC1604.21c.1 ; SPBC1604.21c.1:pep ; SPBC1604.21c .
GeneIDi 2540744.
KEGGi spo:SPBC1604.21c.

Organism-specific databases

PomBasei SPBC1604.21c.

Phylogenomic databases

eggNOGi COG0476.
HOGENOMi HOG000167329.
KOi K03178.
OMAi KFHPVKQ.
OrthoDBi EOG7FZ07P.
PhylomeDBi O94609.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

NextBioi 20801866.
PROi O94609.

Family and domain databases

Gene3Di 1.10.3240.10. 2 hits.
3.40.50.720. 4 hits.
InterProi IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view ]
Pfami PF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view ]
PRINTSi PR01849. UBIQUITINACT.
SMARTi SM00985. UBA_e1_C. 1 hit.
[Graphical view ]
SUPFAMi SSF69572. SSF69572. 2 hits.
TIGRFAMsi TIGR01408. Ube1. 1 hit.
PROSITEi PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe."
    Azad A.K., Tani T., Shiki N., Tsuneyoshi S., Urushiyama S., Ohshima Y.
    Mol. Biol. Cell 8:825-841(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiUBA1_SCHPO
AccessioniPrimary (citable) accession number: O94609
Secondary accession number(s): Q9P7R2, Q9USY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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