Reviewed, UniProtKB/Swiss-Prot O94582 (TRPE_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I

Gene names

Name:	trp3
ORF Names:	SPCC1442.09

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Probable anthranilate synthase component 1		PRO_0000154133
Amino acid modifications
	Modified residue	390	1	Phosphoserine Ref.3
	Modified residue	392	1	Phosphoserine Ref.3
	Modified residue	488	1	Phosphoserine Ref.3
Experimental info
	Sequence conflict	144	1	D → N in BAA13917. Ref.2
	Sequence conflict	156	1	K → Q in BAA13917. Ref.2
	Sequence conflict	263	1	Y → F in BAA13917. Ref.2
	Sequence conflict	305	1	K → E in BAA13917. Ref.2
	Sequence conflict	320	1	K → Q in BAA13917. Ref.2
	Sequence conflict	351	1	M → R in BAA13917. Ref.2
	Sequence conflict	402	1	Y → N in BAA13917. Ref.2
	Sequence conflict	411	1	I → R in BAA13917. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O94582-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 442D522BA59EBA31	FASTA	489	54,960
	10 20 30 40 50 60 MKIYPDLKQV QELAEKHKAN KIPIYGVIPA DMLTPSVAYL KLNQGKKYSF ILESVTQGES 70 80 90 100 110 120 VSRYSFIGSP YRILMANGKT DPLARLEREL KEVKTAPVEG LPSFSGGAVG YVSYDCIKYF 130 140 150 160 170 180 EPTTEMPLED TLGLPEAMFF MTDDLVAFDH AYQTVKIISH VCIQQGRPIE EAYEAAVFKI 190 200 210 220 230 240 NMLKKKLESP EIPLPEQKKV HLGYEAKSNV GEDGYKAFVS NLKEHIFNGD IFQAVPSQRI 250 260 270 280 290 300 ARRTDLHPFN LYRHLRTVNP SPYMFYIHCD DFDIIGASPE LLVKSEHGRI INHPIAGTVP 310 320 330 340 350 360 RGKTKEEDEA YAKDLLASVK DRAEHVMLVD LARNDVSRVC DLDTTSVDKL MTIEKFSHVQ 370 380 390 400 410 420 HLVSQVSGVL RPDKTRFDAF RSIFPAGTVS GSPKVRAIQL VYGLEKEKRG IYAGAVGRWG 430 440 450 460 470 480 YEDDNMDTCI AIRTMVYKDG TVYLQAGGGI VFDSDEQDEY VETLNKLRSN VTAIEETEKL YAEEENSSA

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 126-489. Strain: PR745.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-390; SER-392 AND SER-488, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAA21443.1. D89256 mRNA. Translation: BAA13917.1.
PIR	T40974. T43181.
RefSeq	NP_588323.1.
3D structure databases
HSSP	HSSP built from PDB template 1K0E based on UniProtKB P05041.
ModBase	Search...
Genome annotation databases
GeneID	2538842.
KEGG	spo:SPCC1442.09.
NMPDR	fig|4896.1.peg.661.
Organism-specific databases
GeneDB_Spombe	SPCC1442.09.
Phylogenomic databases
OMA	O94582. RFSILAY.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000246-MON.
BRENDA	4.1.3.27. 653.
Gene expression databases
ArrayExpress	O94582.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR005256. Anth_synthI. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
ProDom	PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00564. trpE_most. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_SCHPO

Accession

Primary (citable) accession number: O94582
Secondary accession number(s): P78905

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)