Reviewed, UniProtKB/Swiss-Prot O94567 (SYNC_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable asparaginyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

ORF Names:

SPBC1773.10c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred by curator. Source: GeneDB_SPombe aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Probable asparaginyl-tRNA synthetase, cytoplasmic		PRO_0000176499

Sequences

	Sequence		Length	Mass (Da)
	O94567-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 934483A10962BCAC	FASTA	568	63,870
	10 20 30 40 50 60 MAGLESKVSD MKIKSDVVFY IDEASGNDET GNGSQTSPFK TAIHALETDA NCTIFVKKNG 70 80 90 100 110 120 SEEFEPITTN ALKKAKKGVE QAAKKKAKAA EAEAAAAARA AAAKEAEAKR LEAAKNIVLK 130 140 150 160 170 180 EPKDAPAAKK IAIIDSTNFR DSRVRVNGWV HRMRTQKGII FIILRDGTGF LQCVLSGKVY 190 200 210 220 230 240 DRASYDFINL GPESTVCLYG VIKELPEGKS APGNHELVVD YYQILHAAPT GEEAFTNRLN 250 260 270 280 290 300 AEAEPSYLLD QRHLVIRGET ASSVLKVRAR ALRAMRDTFE NLKMTEVTPP CMVQTQVEGG 310 320 330 340 350 360 ATLFKFNYYG QDAYLTQSSQ LYLEAALPAL GSVYTIQESF RAEKSLTRRH LSEFTHVEFE 370 380 390 400 410 420 LPFVNFGEFL EIIEEFICQT IDRLLDDPIA TPLIKQLNPD FVKPSRPFMR LSYEDAIKYL 430 440 450 460 470 480 NEHNILTPEG EQHKFGDDIA EAAERKMTDQ INRPIFLTYF PLEIKSFYMK RVVDRPELTE 490 500 510 520 530 540 SVDCLMPNVG EIVGGSMRIS DIQELLAAYK REGIDPAPYY WFTEQRKYGT TEHGGCGLGL 550 560 ERFLAWLCDR YTVRECCLFP RFTERCTP

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA21915.1.
PIR	T39675.
RefSeq	NP_595125.1.
3D structure databases
HSSP	HSSP built from PDB template 1B8A based on UniProtKB Q52428.
ModBase	Search...
Protein-protein interaction databases
STRING	O94567.
Genome annotation databases
GeneID	2540046.
KEGG	spo:SPBC1773.10c.
NMPDR	fig|4896.1.peg.991.
Organism-specific databases
GeneDB_Spombe	SPBC1773.10c.
Phylogenomic databases
OMA	SEYTHIE.
Enzyme and pathway databases
BRENDA	6.1.1.22. 653.
Gene expression databases
ArrayExpress	O94567.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYNC_SCHPO

Accession

Primary (citable) accession number: O94567

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)