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Protein

Anaphase-promoting complex subunit 8

Gene

cut23

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. Has a role in promoting metaphase to anaphase transition via the ubiquitination of specific mitotic substrates.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of mitotic metaphase/anaphase transition Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 8
Alternative name(s):
20S cyclosome/APC complex protein apc8
Cell untimely torn protein 23
Gene namesi
Name:cut23
Synonyms:apc8
ORF Names:SPAC6F12.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.14.
PomBaseiSPAC6F12.14. cut23.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Anaphase-promoting complex subunit 8PRO_0000106282Add
BLAST

Proteomic databases

MaxQBiO94556.

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits: apc1, apc2, nuc2, apc4, apc5, cut9, apc8, apc10, apc11, hcn1, apc13, apc14 and apc15.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
plo1P505285EBI-1116472,EBI-1112601

Protein-protein interaction databases

BioGridi279325. 15 interactions.
IntActiO94556. 3 interactions.
MINTiMINT-4685182.

Structurei

Secondary structure

1
565
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 3617Combined sources
Helixi40 – 5112Combined sources
Helixi79 – 868Combined sources
Helixi88 – 9912Combined sources
Helixi103 – 1097Combined sources
Turni110 – 1123Combined sources
Helixi116 – 13419Combined sources
Helixi155 – 16814Combined sources
Helixi173 – 18513Combined sources
Helixi189 – 20214Combined sources
Helixi207 – 2159Combined sources
Helixi220 – 2278Combined sources
Helixi235 – 24814Combined sources
Helixi253 – 26513Combined sources
Helixi270 – 28314Combined sources
Helixi296 – 2983Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZN3X-ray1.90A19-301[»]
5FTPX-ray3.10A/B19-301[»]
ProteinModelPortaliO94556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati87 – 12034TPR 1Add
BLAST
Repeati173 – 20634TPR 2Add
BLAST
Repeati270 – 30334TPR 3Add
BLAST
Repeati338 – 37134TPR 4Add
BLAST
Repeati373 – 40533TPR 5Add
BLAST
Repeati407 – 43933TPR 6Add
BLAST
Repeati440 – 47334TPR 7Add
BLAST
Repeati475 – 50733TPR 8Add
BLAST

Sequence similaritiesi

Contains 8 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

HOGENOMiHOG000115852.
InParanoidiO94556.
KOiK03355.
OMAiEDEMEWE.
OrthoDBiEOG7KDFKN.
PhylomeDBiO94556.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR007192. APC8.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF04049. ANAPC8. 1 hit.
PF13181. TPR_8. 4 hits.
[Graphical view]
SMARTiSM00028. TPR. 7 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVSLNTMMG VDGLEDQEQL REIRNCLLKC ISECSERGLV YAVRWAAEML
60 70 80 90 100
NGMNPIEMEH IPFSSTPTGE FDLDPDMANE KLLEVEEKNI YLLAKSYFDC
110 120 130 140 150
KEFERAAYTL QNCKSSKSIF LRLYSKYLAG EKKSEEENET LLNTNLTLSS
160 170 180 190 200
TNREFYYISE VLESLHYQGN KDPYLLYLSG VVYRKRKQDS KAIDFLKSCV
210 220 230 240 250
LKAPFFWSAW LELSLSIDSL ETLTTVVSQL PSTHIMTKIF YVYASHELHQ
260 270 280 290 300
VNSSAYEKLA EAEIIFPNSR YLKTQRALLT YDSRDFDEAE SLFENILTND
310 320 330 340 350
PYRLDDMDTY SNVLFVLENK SKLGFLAQVA SSIDKFRPET CSIIGNYYSL
360 370 380 390 400
LSEHEKAVTY FKRALQLNRN YLSAWTLMGH EYVELKNTHA AIESYRLAVD
410 420 430 440 450
VNRKDYRAWY GLGQTYEVLD MHFYALYYFQ RATALRPYDQ RMWQALGNCY
460 470 480 490 500
EKIDRPQEAI KSYKRALLGS QTNSSILVRL GNLYEELQDL NSAASMYKQC
510 520 530 540 550
IKTEETEISP ETIKARIWLA RWELGKKNYR EAELYLSEVL NGDLELEEAK
560
ALLRELRSRM EHSYD
Length:565
Mass (Da):65,855
Last modified:May 1, 2000 - v2
Checksum:i02B4C9CC07D21BB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11101.2.
PIRiT11665.
RefSeqiNP_593300.1. NM_001018730.2.

Genome annotation databases

EnsemblFungiiSPAC6F12.14.1; SPAC6F12.14.1:pep; SPAC6F12.14.
GeneIDi2542880.
KEGGispo:SPAC6F12.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11101.2.
PIRiT11665.
RefSeqiNP_593300.1. NM_001018730.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZN3X-ray1.90A19-301[»]
5FTPX-ray3.10A/B19-301[»]
ProteinModelPortaliO94556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279325. 15 interactions.
IntActiO94556. 3 interactions.
MINTiMINT-4685182.

Proteomic databases

MaxQBiO94556.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6F12.14.1; SPAC6F12.14.1:pep; SPAC6F12.14.
GeneIDi2542880.
KEGGispo:SPAC6F12.14.

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.14.
PomBaseiSPAC6F12.14. cut23.

Phylogenomic databases

HOGENOMiHOG000115852.
InParanoidiO94556.
KOiK03355.
OMAiEDEMEWE.
OrthoDBiEOG7KDFKN.
PhylomeDBiO94556.

Miscellaneous databases

NextBioi20803920.
PROiO94556.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR007192. APC8.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF04049. ANAPC8. 1 hit.
PF13181. TPR_8. 4 hits.
[Graphical view]
SMARTiSM00028. TPR. 7 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses."
    Yamashita Y.M., Nakaseko Y., Kumada K., Nakagawa T., Yanagida M.
    Genes Cells 4:445-463(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes."
    Yoon H.-J., Feoktistova A., Wolfe B.A., Jennings J.L., Link A.J., Gould K.L.
    Curr. Biol. 12:2048-2054(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiAPC8_SCHPO
AccessioniPrimary (citable) accession number: O94556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.