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Protein

Anaphase-promoting complex subunit 8

Gene

cut23

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. Has a role in promoting metaphase to anaphase transition via the ubiquitination of specific mitotic substrates.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of mitotic metaphase/anaphase transition Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 8
Alternative name(s):
20S cyclosome/APC complex protein apc8
Cell untimely torn protein 23
Gene namesi
Name:cut23
Synonyms:apc8
ORF Names:SPAC6F12.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.14.
PomBaseiSPAC6F12.14. cut23.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062821 – 565Anaphase-promoting complex subunit 8Add BLAST565

Proteomic databases

MaxQBiO94556.
PRIDEiO94556.

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits: apc1, apc2, nuc2, apc4, apc5, cut9, apc8, apc10, apc11, hcn1, apc13, apc14 and apc15.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
plo1P505285EBI-1116472,EBI-1112601

Protein-protein interaction databases

BioGridi279325. 15 interactors.
IntActiO94556. 3 interactors.
MINTiMINT-4685182.

Structurei

Secondary structure

1565
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 36Combined sources17
Helixi40 – 51Combined sources12
Helixi79 – 86Combined sources8
Helixi88 – 99Combined sources12
Helixi103 – 109Combined sources7
Turni110 – 112Combined sources3
Helixi116 – 134Combined sources19
Beta strandi150 – 152Combined sources3
Helixi155 – 168Combined sources14
Helixi173 – 185Combined sources13
Helixi189 – 202Combined sources14
Helixi207 – 215Combined sources9
Helixi220 – 227Combined sources8
Helixi235 – 248Combined sources14
Helixi253 – 265Combined sources13
Helixi270 – 283Combined sources14
Helixi296 – 298Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZN3X-ray1.90A19-301[»]
5FTPX-ray3.10A/B19-301[»]
ProteinModelPortaliO94556.
SMRiO94556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati87 – 120TPR 1Add BLAST34
Repeati173 – 206TPR 2Add BLAST34
Repeati270 – 303TPR 3Add BLAST34
Repeati338 – 371TPR 4Add BLAST34
Repeati373 – 405TPR 5Add BLAST33
Repeati407 – 439TPR 6Add BLAST33
Repeati440 – 473TPR 7Add BLAST34
Repeati475 – 507TPR 8Add BLAST33

Sequence similaritiesi

Contains 8 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

HOGENOMiHOG000115852.
InParanoidiO94556.
KOiK03355.
OMAiEDEMEWE.
OrthoDBiEOG092C176G.
PhylomeDBiO94556.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR007192. APC8.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF04049. ANAPC8. 1 hit.
PF13181. TPR_8. 4 hits.
[Graphical view]
SMARTiSM00028. TPR. 7 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVSLNTMMG VDGLEDQEQL REIRNCLLKC ISECSERGLV YAVRWAAEML
60 70 80 90 100
NGMNPIEMEH IPFSSTPTGE FDLDPDMANE KLLEVEEKNI YLLAKSYFDC
110 120 130 140 150
KEFERAAYTL QNCKSSKSIF LRLYSKYLAG EKKSEEENET LLNTNLTLSS
160 170 180 190 200
TNREFYYISE VLESLHYQGN KDPYLLYLSG VVYRKRKQDS KAIDFLKSCV
210 220 230 240 250
LKAPFFWSAW LELSLSIDSL ETLTTVVSQL PSTHIMTKIF YVYASHELHQ
260 270 280 290 300
VNSSAYEKLA EAEIIFPNSR YLKTQRALLT YDSRDFDEAE SLFENILTND
310 320 330 340 350
PYRLDDMDTY SNVLFVLENK SKLGFLAQVA SSIDKFRPET CSIIGNYYSL
360 370 380 390 400
LSEHEKAVTY FKRALQLNRN YLSAWTLMGH EYVELKNTHA AIESYRLAVD
410 420 430 440 450
VNRKDYRAWY GLGQTYEVLD MHFYALYYFQ RATALRPYDQ RMWQALGNCY
460 470 480 490 500
EKIDRPQEAI KSYKRALLGS QTNSSILVRL GNLYEELQDL NSAASMYKQC
510 520 530 540 550
IKTEETEISP ETIKARIWLA RWELGKKNYR EAELYLSEVL NGDLELEEAK
560
ALLRELRSRM EHSYD
Length:565
Mass (Da):65,855
Last modified:May 1, 2000 - v2
Checksum:i02B4C9CC07D21BB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11101.2.
PIRiT11665.
RefSeqiNP_593300.1. NM_001018730.2.

Genome annotation databases

EnsemblFungiiSPAC6F12.14.1; SPAC6F12.14.1:pep; SPAC6F12.14.
GeneIDi2542880.
KEGGispo:SPAC6F12.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11101.2.
PIRiT11665.
RefSeqiNP_593300.1. NM_001018730.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZN3X-ray1.90A19-301[»]
5FTPX-ray3.10A/B19-301[»]
ProteinModelPortaliO94556.
SMRiO94556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279325. 15 interactors.
IntActiO94556. 3 interactors.
MINTiMINT-4685182.

Proteomic databases

MaxQBiO94556.
PRIDEiO94556.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6F12.14.1; SPAC6F12.14.1:pep; SPAC6F12.14.
GeneIDi2542880.
KEGGispo:SPAC6F12.14.

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.14.
PomBaseiSPAC6F12.14. cut23.

Phylogenomic databases

HOGENOMiHOG000115852.
InParanoidiO94556.
KOiK03355.
OMAiEDEMEWE.
OrthoDBiEOG092C176G.
PhylomeDBiO94556.

Miscellaneous databases

PROiO94556.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR007192. APC8.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF04049. ANAPC8. 1 hit.
PF13181. TPR_8. 4 hits.
[Graphical view]
SMARTiSM00028. TPR. 7 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPC8_SCHPO
AccessioniPrimary (citable) accession number: O94556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.