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O94523

- ACO1_SCHPO

UniProt

O94523 - ACO1_SCHPO

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Protein

Probable acyl-CoA desaturase

Gene

SPCC1281.06c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Utilizes O2 and electrons from the reduced cytochrome b5 domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates.By similarity

Catalytic activityi

Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.

Cofactori

Iron.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi390 – 3901Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi416 – 4161Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. stearoyl-CoA 9-desaturase activity Source: PomBase

GO - Biological processi

  1. unsaturated fatty acid biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable acyl-CoA desaturase (EC:1.14.19.1)
Alternative name(s):
Delta(9)-desaturase
Short name:
Delta-9 desaturase
Fatty acid desaturase
Stearoyl-CoA desaturase
Gene namesi
ORF Names:SPCC1281.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome III

Organism-specific databases

PomBaseiSPCC1281.06c.

Subcellular locationi

Membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: PomBase
  2. endoplasmic reticulum membrane Source: PomBase
  3. integral component of membrane Source: UniProtKB-KW
  4. intrinsic component of endoplasmic reticulum membrane Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Probable acyl-CoA desaturasePRO_0000185405Add
BLAST

Proteomic databases

MaxQBiO94523.

Interactioni

Protein-protein interaction databases

BioGridi275336. 4 interactions.
MINTiMINT-4684891.
STRINGi4896.SPCC1281.06c-1.

Structurei

3D structure databases

ProteinModelPortaliO94523.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei62 – 8221HelicalSequence AnalysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence AnalysisAdd
BLAST
Transmembranei205 – 22521HelicalSequence AnalysisAdd
BLAST
Transmembranei230 – 25021HelicalSequence AnalysisAdd
BLAST
Transmembranei305 – 32521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini357 – 43377Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi110 – 1156Histidine box-1
Motifi147 – 1515Histidine box-2
Motifi284 – 2885Histidine box-3

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.By similarity

Sequence similaritiesi

Belongs to the fatty acid desaturase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1398.
HOGENOMiHOG000270353.
InParanoidiO94523.
KOiK00507.
OMAiHITETPL.
OrthoDBiEOG7S2277.
PhylomeDBiO94523.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR005804. Fatty_acid_desaturase-1.
IPR001522. Fatty_acid_desaturase-1_C.
IPR015876. Fatty_acid_desaturase-1_core.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFiPIRSF000345. OLE1. 1 hit.
PRINTSiPR00075. FACDDSATRASE.
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS50255. CYTOCHROME_B5_2. 1 hit.
PS00476. FATTY_ACID_DESATUR_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94523-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAPSATAFS SATTQPTTEG NASMRKRTIP VVPSVPERKW DPKAPKHIQE
60 70 80 90 100
QPWTMQNWWR HLNWLHCMLI FGLPMIAIYG VFTTPLQTKT LIFAIIYYAY
110 120 130 140 150
SGLGITAGYH RLWSHRAYKA KKPLEYFLAA GGAAAFEGSI RWWSRDHRAH
160 170 180 190 200
HRYTDTDKDP YNVKKGFWYA HVGWMIILQN PRRIGRSDVS DLNSDPFVMF
210 220 230 240 250
NHRHFLPIAS FMAFIFPSLF CGLLWGDYRG GYFYAGVCRL VFVHHATFCV
260 270 280 290 300
NSLAHLIGSQ PFDDTNSARN HFITALVTLG EGNHNYHHAF PNDYRNGLRW
310 320 330 340 350
YEYDPTKIFI YIASLFGLAY NLNTFPDNEI QKGIVQQKQK VLDRWRAKLN
360 370 380 390 400
WGIPLEQLPV MEFEDFLEQS KTRPLVLING VVHDMTGFEH PGGQGLLRSA
410 420 430 440 450
FGKDATAAFN GGVYDHTNGA HNLLSTYRVA VVRGGMEVEV WKSGAGAQLP
460 470
MKDTQGQKIV RVGEQITRIQ PPIEAAAAN
Length:479
Mass (Da):54,438
Last modified:May 1, 1999 - v1
Checksum:iED5666E7B35EFCF2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 596TMQNWW → GPCKTV(PubMed:10759889)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAA22827.1.
AB027859 Genomic DNA. Translation: BAA87163.1.
PIRiT40925.
RefSeqiNP_588170.1. NM_001023159.2.

Genome annotation databases

EnsemblFungiiSPCC1281.06c.1; SPCC1281.06c.1:pep; SPCC1281.06c.
GeneIDi2538753.
KEGGispo:SPCC1281.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAA22827.1 .
AB027859 Genomic DNA. Translation: BAA87163.1 .
PIRi T40925.
RefSeqi NP_588170.1. NM_001023159.2.

3D structure databases

ProteinModelPortali O94523.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 275336. 4 interactions.
MINTi MINT-4684891.
STRINGi 4896.SPCC1281.06c-1.

Proteomic databases

MaxQBi O94523.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPCC1281.06c.1 ; SPCC1281.06c.1:pep ; SPCC1281.06c .
GeneIDi 2538753.
KEGGi spo:SPCC1281.06c.

Organism-specific databases

PomBasei SPCC1281.06c.

Phylogenomic databases

eggNOGi COG1398.
HOGENOMi HOG000270353.
InParanoidi O94523.
KOi K00507.
OMAi HITETPL.
OrthoDBi EOG7S2277.
PhylomeDBi O94523.

Miscellaneous databases

NextBioi 20799937.

Family and domain databases

Gene3Di 3.10.120.10. 1 hit.
InterProi IPR009160. Acyl-CoA_deSatase_haem/ster-bd.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR005804. Fatty_acid_desaturase-1.
IPR001522. Fatty_acid_desaturase-1_C.
IPR015876. Fatty_acid_desaturase-1_core.
[Graphical view ]
Pfami PF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000345. OLE1. 1 hit.
PRINTSi PR00075. FACDDSATRASE.
SUPFAMi SSF55856. SSF55856. 1 hit.
PROSITEi PS50255. CYTOCHROME_B5_2. 1 hit.
PS00476. FATTY_ACID_DESATUR_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 54-257, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.

Entry informationi

Entry nameiACO1_SCHPO
AccessioniPrimary (citable) accession number: O94523
Secondary accession number(s): Q9UU24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3