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O94523

- ACO1_SCHPO

UniProt

O94523 - ACO1_SCHPO

Protein

Probable acyl-CoA desaturase

Gene

SPCC1281.06c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Utilizes O2 and electrons from the reduced cytochrome b5 domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates.By similarity

    Catalytic activityi

    Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.

    Cofactori

    Iron.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi390 – 3901Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi416 – 4161Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. stearoyl-CoA 9-desaturase activity Source: PomBase

    GO - Biological processi

    1. unsaturated fatty acid biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable acyl-CoA desaturase (EC:1.14.19.1)
    Alternative name(s):
    Delta(9)-desaturase
    Short name:
    Delta-9 desaturase
    Fatty acid desaturase
    Stearoyl-CoA desaturase
    Gene namesi
    ORF Names:SPCC1281.06c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC1281.06c.

    Subcellular locationi

    Membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: PomBase
    2. endoplasmic reticulum membrane Source: PomBase
    3. integral component of membrane Source: UniProtKB-KW
    4. intrinsic component of endoplasmic reticulum membrane Source: PomBase

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Probable acyl-CoA desaturasePRO_0000185405Add
    BLAST

    Proteomic databases

    MaxQBiO94523.

    Interactioni

    Protein-protein interaction databases

    BioGridi275336. 4 interactions.
    MINTiMINT-4684891.
    STRINGi4896.SPCC1281.06c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO94523.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei62 – 8221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei90 – 11021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei230 – 25021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei305 – 32521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini357 – 43377Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi110 – 1156Histidine box-1
    Motifi147 – 1515Histidine box-2
    Motifi284 – 2885Histidine box-3

    Domaini

    The histidine box domains may contain the active site and/or be involved in metal ion binding.By similarity

    Sequence similaritiesi

    Belongs to the fatty acid desaturase family.Curated
    Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1398.
    HOGENOMiHOG000270353.
    KOiK00507.
    OMAiHITETPL.
    OrthoDBiEOG7S2277.
    PhylomeDBiO94523.

    Family and domain databases

    Gene3Di3.10.120.10. 1 hit.
    InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
    IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR005804. Fatty_acid_desaturase-1.
    IPR001522. Fatty_acid_desaturase-1_C.
    IPR015876. Fatty_acid_desaturase-1_core.
    [Graphical view]
    PfamiPF00173. Cyt-b5. 1 hit.
    PF00487. FA_desaturase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000345. OLE1. 1 hit.
    PRINTSiPR00075. FACDDSATRASE.
    SUPFAMiSSF55856. SSF55856. 1 hit.
    PROSITEiPS50255. CYTOCHROME_B5_2. 1 hit.
    PS00476. FATTY_ACID_DESATUR_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94523-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAPSATAFS SATTQPTTEG NASMRKRTIP VVPSVPERKW DPKAPKHIQE    50
    QPWTMQNWWR HLNWLHCMLI FGLPMIAIYG VFTTPLQTKT LIFAIIYYAY 100
    SGLGITAGYH RLWSHRAYKA KKPLEYFLAA GGAAAFEGSI RWWSRDHRAH 150
    HRYTDTDKDP YNVKKGFWYA HVGWMIILQN PRRIGRSDVS DLNSDPFVMF 200
    NHRHFLPIAS FMAFIFPSLF CGLLWGDYRG GYFYAGVCRL VFVHHATFCV 250
    NSLAHLIGSQ PFDDTNSARN HFITALVTLG EGNHNYHHAF PNDYRNGLRW 300
    YEYDPTKIFI YIASLFGLAY NLNTFPDNEI QKGIVQQKQK VLDRWRAKLN 350
    WGIPLEQLPV MEFEDFLEQS KTRPLVLING VVHDMTGFEH PGGQGLLRSA 400
    FGKDATAAFN GGVYDHTNGA HNLLSTYRVA VVRGGMEVEV WKSGAGAQLP 450
    MKDTQGQKIV RVGEQITRIQ PPIEAAAAN 479
    Length:479
    Mass (Da):54,438
    Last modified:May 1, 1999 - v1
    Checksum:iED5666E7B35EFCF2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 596TMQNWW → GPCKTV(PubMed:10759889)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA22827.1.
    AB027859 Genomic DNA. Translation: BAA87163.1.
    PIRiT40925.
    RefSeqiNP_588170.1. NM_001023159.2.

    Genome annotation databases

    EnsemblFungiiSPCC1281.06c.1; SPCC1281.06c.1:pep; SPCC1281.06c.
    GeneIDi2538753.
    KEGGispo:SPCC1281.06c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA22827.1 .
    AB027859 Genomic DNA. Translation: BAA87163.1 .
    PIRi T40925.
    RefSeqi NP_588170.1. NM_001023159.2.

    3D structure databases

    ProteinModelPortali O94523.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 275336. 4 interactions.
    MINTi MINT-4684891.
    STRINGi 4896.SPCC1281.06c-1.

    Proteomic databases

    MaxQBi O94523.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC1281.06c.1 ; SPCC1281.06c.1:pep ; SPCC1281.06c .
    GeneIDi 2538753.
    KEGGi spo:SPCC1281.06c.

    Organism-specific databases

    PomBasei SPCC1281.06c.

    Phylogenomic databases

    eggNOGi COG1398.
    HOGENOMi HOG000270353.
    KOi K00507.
    OMAi HITETPL.
    OrthoDBi EOG7S2277.
    PhylomeDBi O94523.

    Miscellaneous databases

    NextBioi 20799937.

    Family and domain databases

    Gene3Di 3.10.120.10. 1 hit.
    InterProi IPR009160. Acyl-CoA_deSatase_haem/ster-bd.
    IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR005804. Fatty_acid_desaturase-1.
    IPR001522. Fatty_acid_desaturase-1_C.
    IPR015876. Fatty_acid_desaturase-1_core.
    [Graphical view ]
    Pfami PF00173. Cyt-b5. 1 hit.
    PF00487. FA_desaturase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000345. OLE1. 1 hit.
    PRINTSi PR00075. FACDDSATRASE.
    SUPFAMi SSF55856. SSF55856. 1 hit.
    PROSITEi PS50255. CYTOCHROME_B5_2. 1 hit.
    PS00476. FATTY_ACID_DESATUR_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 54-257, SUBCELLULAR LOCATION.
      Strain: ATCC 38364 / 968.

    Entry informationi

    Entry nameiACO1_SCHPO
    AccessioniPrimary (citable) accession number: O94523
    Secondary accession number(s): Q9UU24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3