Reviewed,
UniProtKB/Swiss-Prot O94523 (ACO1_SCHPO)
Last modified
June 16, 2009.
Version 64.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Probable acyl-CoA desaturase EC=1.14.19.1 Alternative name(s): Stearoyl-CoA desaturase Fatty acid desaturase Delta(9)-desaturase | ||
| Gene names |
| ||
| Organism | Schizosaccharomyces pombe (Fission yeast) [Complete proteome] | ||
| Taxonomic identifier | 4896 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 479 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Utilizes O2 and electrons from the reduced cytochrome b5 domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates By similarity. |
| Catalytic activity | Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O. |
| Cofactor | Iron By similarity. |
| Subcellular location | |
| Domain | The histidine box domains may contain the active site and/or be involved in metal ion binding By similarity. |
| Sequence similarities | Belongs to the fatty acid desaturase family. Contains 1 cytochrome b5 heme-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Electron transport Fatty acid biosynthesis Lipid synthesis Transport |
| Cellular component | Membrane |
| Domain | Transmembrane |
| Ligand | Heme Iron Metal-binding |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW fatty acid biosynthetic processInferred by curator. Source: GeneDB_SPombe transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membraneNon-traceable author statement. Source: GeneDB_SPombe |
| Molecular function | heme binding Inferred from electronic annotation. Source: InterPro stearoyl-CoA 9-desaturase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 479 | 479 | Probable acyl-CoA desaturase | PRO_0000185405 | |||||
Regions | |||||||||
| Transmembrane | 62 – 82 | 21 | Potential | ||||||
| Transmembrane | 90 – 110 | 21 | Potential | ||||||
| Transmembrane | 205 – 225 | 21 | Potential | ||||||
| Transmembrane | 230 – 250 | 21 | Potential | ||||||
| Transmembrane | 305 – 325 | 21 | Potential | ||||||
| Domain | 357 – 433 | 77 | Cytochrome b5 heme-binding | ||||||
| Motif | 110 – 115 | 6 | Histidine box-1 | ||||||
| Motif | 147 – 151 | 5 | Histidine box-2 | ||||||
| Motif | 284 – 288 | 5 | Histidine box-3 | ||||||
Sites | |||||||||
| Metal binding | 390 | 1 | Iron (heme axial ligand) By similarity | ||||||
| Metal binding | 416 | 1 | Iron (heme axial ligand) By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 54 – 59 | 6 | TMQNWW → GPCKTV Ref.2 | ||||||
Sequences
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References
| [1] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972. |
| [2] | "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 54-257, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968. |
Cross-references
Sequence databases | |
|---|---|
| CU329672 Genomic DNA. Translation: CAA22827.1. AB027859 Genomic DNA. Translation: BAA87163.1. | |
| PIR | T40925. |
| RefSeq | NP_588170.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1AWP based on UniProtKB P04166. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2538753. |
| KEGG | spo:SPCC1281.06c. |
| NMPDR | fig|4896.1.peg.508. |
Organism-specific databases | |
| GeneDB_Spombe | SPCC1281.06c. |
Phylogenomic databases | |
| OMA | O94523. HRIHHRY. |
Enzyme and pathway databases | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-000109-MON. |
| BRENDA | 1.14.19.1. 653. |
Gene expression databases | |
| ArrayExpress | O94523. |
Family and domain databases | |
| InterPro | IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR005804. Fatty_acid_desaturase-1. IPR001522. Fatty_acid_desaturase-1_C. IPR015876. Fatty_acid_desaturase-1_core. IPR009160. OLE1_hae_ster_bd. [Graphical view] |
| Gene3D | G3DSA:3.10.120.10. Cyt_B5. 1 hit. |
| Pfam | PF00173. Cyt-b5. 1 hit. PF00487. FA_desaturase. 1 hit. [Graphical view] |
| PIRSF | PIRSF000345. OLE1. 1 hit. |
| PRINTS | PR00075. FACDDSATRASE. |
| ProDom | PD000612. Cyt_B5. 1 hit. PD002221. Desaturase. 1 hit. PD001081. FA_desat_sub. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00191. CYTOCHROME_B5_1. False negative. PS50255. CYTOCHROME_B5_2. 1 hit. PS00476. FATTY_ACID_DESATUR_1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ACO1_SCHPO | ||||||||
| Accession | Primary (citable) accession number: O94523 Secondary accession number(s): Q9UU24 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


