O94517 (PSA1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable proteasome subunit alpha type-1
EC=3.4.25.1

Gene names

ORF Names:

SPBC646.16

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.2.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: PomBase regulation of mitotic cell cycle Non-traceable author statement. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.2. Source: PomBase nucleus Inferred from direct assay Ref.2. Source: PomBase proteasome core complex, alpha-subunit complex Inferred from sequence or structural similarity. Source: PomBase
Molecular_function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Probable proteasome subunit alpha type-1		PRO_0000124140

Sequences

	Sequence		Length	Mass (Da)
	O94517 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D8F66AE6155CCD59	FASTA	244	26,539
	10 20 30 40 50 60 MSQARGFDRT ITVFSPEGRL YQVEYAFKAF NNAGITSVGV TGKNCACVIS QKKVPDKLID 70 80 90 100 110 120 ASTVKHVFPI TKGIGCVMTG SIADARAQVS RARSEAAEFE YKNGYPMPCD VLAKRMANIA 130 140 150 160 170 180 QVSTQRAAMR PLGVAMTLVA VDDEIGPSLF KLDPAGFYIG YKATSAGPKQ TETINWLEKR 190 200 210 220 230 240 FKKDGIPSNL TDTVETGIQA LMSSLSTDFK STELQIGVVE DDKPFRVLSV EEIEAHLQSI AEKD

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA22820.1.
PIR	T40592.
RefSeq	NP_595374.1. NM_001021282.2.
3D structure databases
ProteinModelPortal	O94517.
SMR	O94517. Positions 4-243.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	277654. 10 interactions.
MINT	MINT-4684852.
STRING	4896.SPBC646.16-1.
Protein family/group databases
MEROPS	T01.971.
Proteomic databases
MaxQB	O94517.
PaxDb	O94517.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC646.16.1; SPBC646.16.1:pep; SPBC646.16.
GeneID	2541139.
KEGG	spo:SPBC646.16.
Organism-specific databases
PomBase	SPBC646.16.
Phylogenomic databases
eggNOG	COG0638.
HOGENOM	HOG000091084.
KO	K02730.
OMA	MSRTSYD.
OrthoDB	EOG7SBP0C.
PhylomeDB	O94517.
Family and domain databases
Gene3D	3.60.20.10. 1 hit.
InterPro	IPR029055. Ntn_hydrolases_N. IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
SUPFAM	SSF56235. SSF56235. 1 hit.
PROSITE	PS00388. PROTEASOME_ALPHA_1. 1 hit. PS51475. PROTEASOME_ALPHA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802252.
PRO	O94517.

Entry information

Entry name

PSA1_SCHPO

Accession

Primary (citable) accession number: O94517

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 1999
Last modified:	June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program