ID   YN67_SCHPO              Reviewed;         295 AA.
AC   O94511;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   16-JUN-2009, entry version 42.
DE   RecName: Full=Putative enoyl reductase C646.07c;
DE            EC=1.3.1.38;
GN   ORFNames=SPBC646.07c;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the
RT   fission yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- CATALYTIC ACTIVITY: Acyl-CoA + NADP(+) = trans-2,3-dehydroacyl-CoA
CC       + NADPH.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
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DR   EMBL; CU329671; CAA22811.1; -; Genomic_DNA.
DR   PIR; T40583; T40583.
DR   RefSeq; NP_595365.1; -.
DR   GeneID; 2541070; -.
DR   KEGG; spo:SPBC646.07c; -.
DR   NMPDR; fig|4896.1.peg.1231; -.
DR   GeneDB_Spombe; SPBC646.07c; -.
DR   OMA; O94511; KSHPQWY.
DR   BRENDA; 1.3.1.38; 653.
DR   ArrayExpress; O94511; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016631; F:enoyl-[acyl-carrier-protein] reductase acti...; NAS:GeneDB_SPombe.
DR   GO; GO:0019166; F:trans-2-enoyl-CoA reductase (NADPH) activity; IEA:EC.
DR   GO; GO:0030497; P:fatty acid elongation; NAS:GeneDB_SPombe.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
DR   Pfam; PF02544; Steroid_dh; 1.
DR   PROSITE; PS50244; S5A_REDUCTASE; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Endoplasmic reticulum; Lipid synthesis; Membrane;
KW   NADP; Oxidoreductase; Transmembrane.
FT   CHAIN         1    295       Putative enoyl reductase C646.07c.
FT                                /FTId=PRO_0000317698.
FT   TOPO_DOM      1     84       Cytoplasmic (Potential).
FT   TRANSMEM     85    105       Potential.
FT   TOPO_DOM    106    157       Lumenal (Potential).
FT   TRANSMEM    158    178       Potential.
FT   TOPO_DOM    179    186       Cytoplasmic (Potential).
FT   TRANSMEM    187    207       Potential.
FT   TOPO_DOM    208    223       Lumenal (Potential).
FT   TRANSMEM    224    246       Potential.
FT   TOPO_DOM    247    250       Cytoplasmic (Potential).
FT   TRANSMEM    251    268       Potential.
FT   TOPO_DOM    269    295       Lumenal (Potential).
SQ   SEQUENCE   295 AA;  34640 MW;  B4181D13E0F3C333 CRC64;
     MSITLSSRGR KIRKLPKSLE FPLDGSIDKL RDEVSSVTRL PVERLRFSTA DGTTLLPNTT
     LRKYGVGPGA TIWVKDLGPQ IGWRTVFMIE YLGPLVIHLF FILNYKWIYR KDYNLCLNQK
     IAFVLVMLHF MKREYESIFV HRFSLATMPL RNIFKNCAHY HLLSGLFLAY FIYGPWHAND
     YIKPNHLLFL IVGWAFAVLS NFRTHIILRD LRPAGSKKRV IPTGYGFNLV SFPNYFFESL
     GWLFFALLTK SWASWIFLFV GSAQMFVWAK KKHARYLKEF PNYPRSRKIM IPFFL
//