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Protein

Elongation factor 3

Gene

tef3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The main role of EF-3 may be to transduce nucleoside triphosphate energy into mechanical energy for translocation during translation. EF-3 stimulates EF-1-alpha-dependent binding of aminoacyl-tRNA to the ribosome.

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi467 – 4748ATP 1PROSITE-ProRule annotation
Nucleotide bindingi707 – 7148ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 3
Short name:
EF-3
Gene namesi
Name:tef3
Synonyms:ef3
ORF Names:SPCC417.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC417.08.
PomBaseiSPCC417.08. tef3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Elongation factor 3PRO_0000093457Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei714 – 7141Phosphoserine1 Publication
Modified residuei768 – 7681Phosphoserine1 Publication
Modified residuei783 – 7831Phosphoserine1 Publication
Modified residuei1043 – 10431Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94489.

PTM databases

iPTMnetiO94489.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi275863. 7 interactions.
DIPiDIP-59126N.
MINTiMINT-4684611.

Structurei

3D structure databases

ProteinModelPortaliO94489.
SMRiO94489. Positions 5-984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati48 – 8538HEAT 1Add
BLAST
Repeati91 – 12838HEAT 2Add
BLAST
Repeati130 – 16738HEAT 3Add
BLAST
Repeati171 – 20838HEAT 4Add
BLAST
Repeati210 – 24637HEAT 5Add
BLAST
Repeati247 – 28438HEAT 6Add
BLAST
Domaini411 – 647237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini673 – 1000328ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi427 – 4337Poly-Glu
Compositional biasi1020 – 103213Lys-rich (basic)Add
BLAST

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation
Contains 6 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000180957.
InParanoidiO94489.
KOiK03235.
OMAiQHIETEG.
OrthoDBiEOG7Q2NDN.
PhylomeDBiO94489.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015688. Elongation_fac_3.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19211:SF5. PTHR19211:SF5. 2 hits.
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF52540. SSF52540. 3 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKSENKQS VKALEELLKH LTVCDAAEEA DAAKALASFV NGPIEEQDAP
60 70 80 90 100
SQVFSAISKQ LNDKNATARE RVLKGLEAVA NHGSVAADVE PYLVELLPAV
110 120 130 140 150
IAKVADKQNA VRDAAIAASK AIVRCTTPYA VKAIVPSVLE SIHTTGKWNE
160 170 180 190 200
KMNSLQLLDV LVEVAPSQLS YSLPQIIPVV SESMWDTKAE VKKQSKETMT
210 220 230 240 250
KVCTLIANAD IDRFIPELIN CIAHPEEVPE TIHSLGATTF VTEVQAPTLS
260 270 280 290 300
IMVPLLARGL NERSTPIKRK TAVIIDNMSK LVEDPQVVAP FLPKLLPGLY
310 320 330 340 350
HIKDTIGDPE CRSVVQRAIT TLERVGNVVD GKIPEVSTAA NPEVCLETLK
360 370 380 390 400
AVLGEIKVPT NEEVIAKYVA NIAAQLVEEK DNENESWVLN ITPYLTAFID
410 420 430 440 450
EAHIHKIVEQ LRTRSIAKIP GGASHAEEEE EGEDLCNCEF SLAYGAKILL
460 470 480 490 500
NRTRLRLKRG RRYGLCGPNG SGKSTLMRAI VNGQVEGFPT HLRTVYVEHD
510 520 530 540 550
IDESEADTPS VDFILQDPAV PIKDRDEIVK ALKENSFTDE LINMPIGSLS
560 570 580 590 600
GGWKMKLALT RAMFKNPDIL LLDEPTNHLD VVNVAWLENF LVNQKDVSSI
610 620 630 640 650
IVSHDSGFLD HVVQAIIHYE RFKLRKYLGN MSEFVKKVPS AKSYYELGAS
660 670 680 690 700
EMEFKFPEPG FLEGVKTKQR AIIKVQHMSF QYPGTSKPQL NDISFQVSLS
710 720 730 740 750
SRIAVIGPNG AGKSTLIKVL TGELLPTVGE IYQHENCRIA YVAQAAFTHL
760 770 780 790 800
GHHPDKTPSE YIQWRFQSGE DLEAMDKASR VISEADEEAM KNKIFKIEGT
810 820 830 840 850
QRKILGIHSR RKLKNSYEYE CSFLVGENIG MKSERWVPMM SSDNAWLPRG
860 870 880 890 900
ELMETHAKMV AEVDRAEALK SGQFRPLVRK EIEEHCSLLG LDAELVSHSR
910 920 930 940 950
IKGLSGGQKV KLVLAACTWL RPHVIVLDEP TNYLDRDSLG ALSKGLKNFG
960 970 980 990 1000
GGVVLVTHSR EFTEGLTEEV WAVNNGHMTP SGHNWVSGQG SGPRIQEKEE
1010 1020 1030 1040
GDTFDAFGNK IEKAKKAKKL TGAELRKKKK ERMARRKAGL EVSDDEL
Length:1,047
Mass (Da):115,802
Last modified:May 1, 1999 - v1
Checksum:i10F5F10DB2E867F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti770 – 7712ED → DH in BAA11573 (PubMed:9099890).Curated
Sequence conflicti777 – 7771K → P in BAA11573 (PubMed:9099890).Curated
Sequence conflicti800 – 8023TQR → NSS in BAA11573 (PubMed:9099890).Curated
Sequence conflicti950 – 9501G → R in BAA11573 (PubMed:9099890).Curated
Sequence conflicti959 – 9591S → Y in BAA33896 (PubMed:10361693).Curated
Sequence conflicti962 – 9621F → I in BAA13887 (PubMed:9501991).Curated
Sequence conflicti972 – 9721A → S in BAA13887 (PubMed:9501991).Curated
Sequence conflicti977 – 9771H → N in BAA13887 (PubMed:9501991).Curated
Sequence conflicti982 – 9821G → V in BAA13887 (PubMed:9501991).Curated
Sequence conflicti988 – 9881G → V in BAA13887 (PubMed:9501991).Curated
Sequence conflicti1001 – 10022GD → AE in BAA13887 (PubMed:9501991).Curated
Sequence conflicti1009 – 10146NKIEKA → TKNEKS in BAA13887 (PubMed:9501991).Curated
Sequence conflicti1023 – 10231A → S in BAA13887 (PubMed:9501991).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA22654.1.
D89226 mRNA. Translation: BAA13887.1.
D82575 mRNA. Translation: BAA11573.1.
AB018538 Genomic DNA. Translation: BAA33896.1.
PIRiT41343.
T43058.
T43434.
RefSeqiNP_588285.1. NM_001023275.2.

Genome annotation databases

EnsemblFungiiSPCC417.08.1; SPCC417.08.1:pep; SPCC417.08.
GeneIDi2539295.
KEGGispo:SPCC417.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA22654.1.
D89226 mRNA. Translation: BAA13887.1.
D82575 mRNA. Translation: BAA11573.1.
AB018538 Genomic DNA. Translation: BAA33896.1.
PIRiT41343.
T43058.
T43434.
RefSeqiNP_588285.1. NM_001023275.2.

3D structure databases

ProteinModelPortaliO94489.
SMRiO94489. Positions 5-984.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275863. 7 interactions.
DIPiDIP-59126N.
MINTiMINT-4684611.

PTM databases

iPTMnetiO94489.

Proteomic databases

MaxQBiO94489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC417.08.1; SPCC417.08.1:pep; SPCC417.08.
GeneIDi2539295.
KEGGispo:SPCC417.08.

Organism-specific databases

EuPathDBiFungiDB:SPCC417.08.
PomBaseiSPCC417.08. tef3.

Phylogenomic databases

HOGENOMiHOG000180957.
InParanoidiO94489.
KOiK03235.
OMAiQHIETEG.
OrthoDBiEOG7Q2NDN.
PhylomeDBiO94489.

Enzyme and pathway databases

UniPathwayiUPA00345.

Miscellaneous databases

PROiO94489.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015688. Elongation_fac_3.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19211:SF5. PTHR19211:SF5. 2 hits.
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF52540. SSF52540. 3 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 731-1047.
    Strain: PR745.
  3. "Comprehensive cloning of Schizosaccharomyces pombe genes encoding translation elongation factors."
    Mita K., Morimyo M., Ito K., Sugaya K., Ebihara K., Hongo E., Higashi T., Hirayama Y., Nakamura Y.
    Gene 187:259-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 749-1047.
    Strain: 972 / ATCC 24843.
  4. "Detection and analysis of translation elongation factor 3 genes from various yeasts."
    Uritani M., Shoumura Y., Yamada S.
    Biosci. Biotechnol. Biochem. 63:769-772(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 450-962, GENE NAME.
    Strain: JY741.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-714; SER-768; SER-783 AND SER-1043, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEF3_SCHPO
AccessioniPrimary (citable) accession number: O94489
Secondary accession number(s): P78876, Q10266, Q9UUL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.