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Protein

Vacuolar membrane protease

Gene

SPCC1919.12c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May be involved in vacuolar sorting and osmoregulation.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi147Zinc 1; catalyticBy similarity1
Metal bindingi159Zinc 1; catalyticBy similarity1
Metal bindingi159Zinc 2; catalyticBy similarity1
Active sitei191Proton acceptorBy similarity1
Metal bindingi192Zinc 2; catalyticBy similarity1
Metal bindingi217Zinc 1; catalyticBy similarity1
Sitei291Transition state stabilizerBy similarity1
Metal bindingi292Zinc 2; catalyticBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar membrane proteaseBy similarity (EC:3.4.-.-Curated)
Alternative name(s):
FXNA-related family protease 1By similarity
Gene namesi
ORF Names:SPCC1919.12cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1919.12c.
PomBaseiSPCC1919.12c.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicBy similarityAdd BLAST16
Transmembranei17 – 37Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini38 – 347VacuolarBy similarityAdd BLAST310
Transmembranei348 – 368Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini369 – 386CytoplasmicBy similarityAdd BLAST18
Transmembranei387 – 407Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini408 – 417VacuolarBy similarity10
Transmembranei418 – 438Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini439 – 456CytoplasmicBy similarityAdd BLAST18
Transmembranei457 – 477Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini478 – 484VacuolarBy similarity7
Transmembranei485 – 505Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini506 – 566CytoplasmicBy similarityAdd BLAST61
Transmembranei567 – 587Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini588 – 608VacuolarBy similarityAdd BLAST21
Transmembranei609 – 629Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini630 – 636CytoplasmicBy similarity7
Transmembranei637 – 657Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini658 – 843VacuolarBy similarityAdd BLAST186

GO - Cellular componenti

  • cytosol Source: PomBase
  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741391 – 843Vacuolar membrane proteaseAdd BLAST843

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi96N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi109N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi117N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi209N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi275N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi322N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi677N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi703N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi707N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi754N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi788N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO94479.
PRIDEiO94479.

Interactioni

Protein-protein interaction databases

BioGridi275729. 4 interactors.
MINTiMINT-4684521.

Structurei

3D structure databases

ProteinModelPortaliO94479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M28 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiO94479.
OrthoDBiEOG092C22R5.
PhylomeDBiO94479.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNSRRHIFE RICAKAFQSS LTCSIFGFTV LLILYLLDWK RIAQVPGPNL
60 70 80 90 100
LKDLEFQRAW NDLEYISSLP HPYNSKQNEH VRSYILKSMR ELEATNQSYI
110 120 130 140 150
TVIDDTLTNI TFESTDNDTL TYFEGDNILV KFEGKSKDLF PILLSAHFDS
160 170 180 190 200
VSTGYGATDD GMGVATVMAI ARYYAKNQPN RDLIININNA EEDYLFGAKA
210 220 230 240 250
FASHKLSKNV TAFVNLEGAG SGGKAMLFRS SNGHVSSAYF KGNHYPLASI
260 270 280 290 300
LGNDFFKRGV IRSQTDYIVY EKMHNHTAGL DIAFYENRDI YHTRKDDINH
310 320 330 340 350
LMPSSLRHMM YTASNAVKNL LNDSKSDLTK FRKPMFFLAF GKYWQLNLPI
360 370 380 390 400
YQVLNIIFAV ICPIVLLLTL IRFPSLYEQL KKPRYTVCFV VSCIFVSIFD
410 420 430 440 450
TLTVLLLTWI NPYVINSHTG LILALFYLTN LIALAFSFRA AATHSKLSSE
460 470 480 490 500
DLSSIEIVFI WYAQILWYLV FIVSVILSIY FQLGSTYWVT LSYLCTFTCC
510 520 530 540 550
IMTIIRINYF VDNVVTTQTT HEEDALIGSS INTSSHQHYG STLNSTPHRR
560 570 580 590 600
NSIALSNRAH VKLIDNIWTV IYFIFNVPFP VFLCYDILVE TILPAGSQTL
610 620 630 640 650
TDSVFSSKLY KLVIFVVFLS LVNSGPFIFR ALSKKSLAVL TMLWITLFVQ
660 670 680 690 700
ALSVNPFTES APLKLSFVQM YDMDRMNNTV YVKNISPFTQ DVLSLNPHFL
710 720 730 740 750
FSNGSCNTSL CYYESTDPDF GGLKTPMSIH IEREKHQLDI SINSGSKWCY
760 770 780 790 800
VDFNTSVFFE AINGNSISGM YSSVRMGQRS FYAPYTLNLT ITEVVKAEVT
810 820 830 840
CLYDDIHEGI IPAYNTFVEH LPSWVAGVKA STGLLKVKSS IVI
Length:843
Mass (Da):95,647
Last modified:May 1, 1999 - v1
Checksum:i80E1A404253574E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti521 – 528HEEDALIG → IQRRRTDW in BAA87092 (PubMed:10759889).Curated8
Sequence conflicti624 – 639SGPFI…KSLAV → QWTFHISCLVKKVACC in BAA87121 (PubMed:10759889).CuratedAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA22643.1.
AB027788 Genomic DNA. Translation: BAA87092.1.
AB027817 Genomic DNA. Translation: BAA87121.1.
PIRiT41237.
RefSeqiNP_588494.1. NM_001023484.2.

Genome annotation databases

EnsemblFungiiSPCC1919.12c.1; SPCC1919.12c.1:pep; SPCC1919.12c.
GeneIDi2539157.
KEGGispo:SPCC1919.12c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA22643.1.
AB027788 Genomic DNA. Translation: BAA87092.1.
AB027817 Genomic DNA. Translation: BAA87121.1.
PIRiT41237.
RefSeqiNP_588494.1. NM_001023484.2.

3D structure databases

ProteinModelPortaliO94479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275729. 4 interactors.
MINTiMINT-4684521.

Proteomic databases

MaxQBiO94479.
PRIDEiO94479.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1919.12c.1; SPCC1919.12c.1:pep; SPCC1919.12c.
GeneIDi2539157.
KEGGispo:SPCC1919.12c.

Organism-specific databases

EuPathDBiFungiDB:SPCC1919.12c.
PomBaseiSPCC1919.12c.

Phylogenomic databases

InParanoidiO94479.
OrthoDBiEOG092C22R5.
PhylomeDBiO94479.

Miscellaneous databases

PROiO94479.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFF1_SCHPO
AccessioniPrimary (citable) accession number: O94479
Secondary accession number(s): Q9UU46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: May 1, 1999
Last modified: October 5, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.