Reviewed, UniProtKB/Swiss-Prot O94468 (MAG2_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable DNA-3-methyladenine glycosylase 2
    EC=3.2.2.21
Alternative name(s):
    3-methyladenine DNA glycosidase 2
    3MEA DNA glycosylase 2

Gene names

ORF Names:

SPBC23G7.11

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions By similarity.
Catalytic activity	Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.
Sequence similarities	Belongs to the alkylbase DNA glycosidase alkA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	alkylbase DNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Probable DNA-3-methyladenine glycosylase 2		PRO_0000194882
Sites
	Active site	162	1	Proton acceptor By similarity
	Site	142	1	Determinant for substrate specificity and/or activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94468-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 21E7E34A181D80EE	FASTA	213	24,292
	10 20 30 40 50 60 MSKDSDYKRA EKHLSSIDNK WSSLVKKVGP CTLTPHPEHA PYEGIIRAIT SQKLSDAATN 70 80 90 100 110 120 SIINKFCTQC SDNDEFPTPK QIMETDVETL HECGFSKLKS QEIHIVAEAA LNKQIPSKSE 130 140 150 160 170 180 IEKMSEEELM ESLSKIKGVK RWTIEMYSIF TLGRLDIMPA DDSTLKNEAK EFFGLSSKPQ 190 200 210 TEEVEKLTKP CKPYRTIAAW YLWQIPKLHR KGQ

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA22627.1.
PIR	T39957.
RefSeq	NP_595869.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O94468.
Genome annotation databases
GeneID	2540646.
KEGG	spo:SPBC23G7.11.
NMPDR	fig|4896.1.peg.1735.
Organism-specific databases
GeneDB_Spombe	SPBC23G7.11.
Phylogenomic databases
OMA	DIELREC.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003939-MON.
BRENDA	3.2.2.21. 653.
Gene expression databases
ArrayExpress	O94468.
Family and domain databases
InterPro	IPR000035. Alkylbase_DNA_glycsylse_CS. IPR003265. HhH-GPD_domain. [Graphical view]
Pfam	PF00730. HhH-GPD. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. [Graphical view]
PROSITE	PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAG2_SCHPO

Accession

Primary (citable) accession number: O94468

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)