O94468 (MAG2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable DNA-3-methyladenine glycosylase 2
EC=3.2.2.21

Alternative name(s):
3-methyladenine DNA glycosidase 2
3MEA DNA glycosylase 2

Gene names

ORF Names:

SPBC23G7.11

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions By similarity.
Catalytic activity	Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.
Sequence similarities	Belongs to the alkylbase DNA glycosidase AlkA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA dealkylation involved in DNA repair Inferred from sequence or structural similarity. Source: GeneDB_Spombe base-excision repair, AP site formation Inferred from genetic interaction. Source: GeneDB_Spombe
Cellular component	nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	DNA-3-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-3-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Probable DNA-3-methyladenine glycosylase 2		PRO_0000194882
Sites
	Active site	162	1	Proton acceptor By similarity
	Site	142	1	Determinant for substrate specificity and/or activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94468 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 21E7E34A181D80EE	FASTA	213	24,292
	10 20 30 40 50 60 MSKDSDYKRA EKHLSSIDNK WSSLVKKVGP CTLTPHPEHA PYEGIIRAIT SQKLSDAATN 70 80 90 100 110 120 SIINKFCTQC SDNDEFPTPK QIMETDVETL HECGFSKLKS QEIHIVAEAA LNKQIPSKSE 130 140 150 160 170 180 IEKMSEEELM ESLSKIKGVK RWTIEMYSIF TLGRLDIMPA DDSTLKNEAK EFFGLSSKPQ 190 200 210 TEEVEKLTKP CKPYRTIAAW YLWQIPKLHR KGQ

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA22627.1.
PIR	T39957.
RefSeq	NP_595869.1. NM_001021775.1.
3D structure databases
ProteinModelPortal	O94468.
ModBase	Search...
Protein-protein interaction databases
STRING	O94468.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC23G7.11.1; SPBC23G7.11.1:pep; SPBC23G7.11.
GeneID	2540646.
KEGG	spo:SPBC23G7.11.
NMPDR	fig|4896.1.peg.1735.
Organism-specific databases
GeneDB_Spombe	SPBC23G7.11.
Phylogenomic databases
eggNOG	fuNOG07498.
GeneTree	EFGT00050000005429.
HOGENOM	HBG544181.
OMA	LYLWEIS.
OrthoDB	EOG43248C.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003939-MONOMER.
Gene expression databases
ArrayExpress	O94468.
Family and domain databases
InterPro	IPR000035. Alkylbase_DNA_glycsylse_CS. IPR011257. DNA_glycosylase. IPR003265. HhH-GPD_domain. IPR023170. HTH_base_excis_C. [Graphical view]
Gene3D	G3DSA:1.10.340.30. DNA_glycosylase. 1 hit. G3DSA:1.10.1670.10. HTH_base_excis_C. 1 hit.
KO	K01247.
Pfam	PF00730. HhH-GPD. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. [Graphical view]
SUPFAM	SSF48150. DNA_glycsylse. 1 hit.
PROSITE	PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAG2_SCHPO

Accession

Primary (citable) accession number: O94468

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program