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Protein

Probable DNA-3-methyladenine glycosylase 2

Gene

mag2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Invlved in base excision repair of methyl methanesulfonate-damaged DNA by hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.1 Publication

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei142 – 1421Determinant for substrate specificity and/or activityBy similarity
Active sitei162 – 1621Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: GO_Central
  • base-excision repair, AP site formation Source: PomBase
  • DNA dealkylation involved in DNA repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA-3-methyladenine glycosylase 2 (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase 2
3MEA DNA glycosylase 2
Gene namesi
Name:mag2
ORF Names:SPBC23G7.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC23G7.11.
PomBaseiSPBC23G7.11. mag2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Probable DNA-3-methyladenine glycosylase 2PRO_0000194882Add
BLAST

Proteomic databases

MaxQBiO94468.

Interactioni

Protein-protein interaction databases

BioGridi277171. 5 interactions.
MINTiMINT-4684434.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Turni15 – 173Combined sources
Helixi19 – 2810Combined sources
Helixi41 – 5010Combined sources
Turni51 – 533Combined sources
Helixi56 – 7015Combined sources
Beta strandi72 – 754Combined sources
Helixi79 – 835Combined sources
Helixi87 – 915Combined sources
Turni92 – 943Combined sources
Helixi97 – 11115Combined sources
Helixi118 – 1236Combined sources
Helixi126 – 1338Combined sources
Helixi141 – 15010Combined sources
Helixi163 – 17210Combined sources
Helixi181 – 1877Combined sources
Helixi189 – 1913Combined sources
Helixi195 – 2039Combined sources
Helixi204 – 2074Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B21X-ray1.45A1-213[»]
4B22X-ray1.90A1-213[»]
4B23X-ray2.00A1-213[»]
4B24X-ray2.30A1-213[»]
4HSBX-ray1.90A1-213[»]
ProteinModelPortaliO94468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0122.
HOGENOMiHOG000261908.
InParanoidiO94468.
OMAiQLCEKWK.
OrthoDBiEOG78SQVJ.
PhylomeDBiO94468.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKDSDYKRA EKHLSSIDNK WSSLVKKVGP CTLTPHPEHA PYEGIIRAIT
60 70 80 90 100
SQKLSDAATN SIINKFCTQC SDNDEFPTPK QIMETDVETL HECGFSKLKS
110 120 130 140 150
QEIHIVAEAA LNKQIPSKSE IEKMSEEELM ESLSKIKGVK RWTIEMYSIF
160 170 180 190 200
TLGRLDIMPA DDSTLKNEAK EFFGLSSKPQ TEEVEKLTKP CKPYRTIAAW
210
YLWQIPKLHR KGQ
Length:213
Mass (Da):24,292
Last modified:May 1, 1999 - v1
Checksum:i21E7E34A181D80EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22627.1.
PIRiT39957.
RefSeqiNP_595869.1. NM_001021775.2.

Genome annotation databases

EnsemblFungiiSPBC23G7.11.1; SPBC23G7.11.1:pep; SPBC23G7.11.
GeneIDi2540646.
KEGGispo:SPBC23G7.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22627.1.
PIRiT39957.
RefSeqiNP_595869.1. NM_001021775.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B21X-ray1.45A1-213[»]
4B22X-ray1.90A1-213[»]
4B23X-ray2.00A1-213[»]
4B24X-ray2.30A1-213[»]
4HSBX-ray1.90A1-213[»]
ProteinModelPortaliO94468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277171. 5 interactions.
MINTiMINT-4684434.

Proteomic databases

MaxQBiO94468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC23G7.11.1; SPBC23G7.11.1:pep; SPBC23G7.11.
GeneIDi2540646.
KEGGispo:SPBC23G7.11.

Organism-specific databases

EuPathDBiFungiDB:SPBC23G7.11.
PomBaseiSPBC23G7.11. mag2.

Phylogenomic databases

eggNOGiCOG0122.
HOGENOMiHOG000261908.
InParanoidiO94468.
OMAiQLCEKWK.
OrthoDBiEOG78SQVJ.
PhylomeDBiO94468.

Miscellaneous databases

NextBioi20801770.
PROiO94468.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  3. "Involvement of 3-methyladenine DNA glycosylases Mag1p and Mag2p in base excision repair of methyl methanesulfonate-damaged DNA in the fission yeast Schizosaccharomyces pombe."
    Kanamitsu K., Tanihigashi H., Tanita Y., Inatani S., Ikeda S.
    Genes Genet. Syst. 82:489-494(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMAG2_SCHPO
AccessioniPrimary (citable) accession number: O94468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.