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Protein

Probable DNA-3-methyladenine glycosylase 2

Gene

mag2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Invlved in base excision repair of methyl methanesulfonate-damaged DNA by hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.1 Publication

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei142Determinant for substrate specificity and/or activityBy similarity1
Active sitei162Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair, AP site formation Source: PomBase
  • DNA dealkylation involved in DNA repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA-3-methyladenine glycosylase 2 (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase 2
3MEA DNA glycosylase 2
Gene namesi
Name:mag2
ORF Names:SPBC23G7.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC23G7.11.
PomBaseiSPBC23G7.11. mag2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
  • protein-DNA complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948821 – 213Probable DNA-3-methyladenine glycosylase 2Add BLAST213

Proteomic databases

MaxQBiO94468.
PRIDEiO94468.

Interactioni

Protein-protein interaction databases

BioGridi277171. 5 interactors.
MINTiMINT-4684434.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Turni15 – 17Combined sources3
Helixi19 – 28Combined sources10
Helixi41 – 50Combined sources10
Turni51 – 53Combined sources3
Helixi56 – 70Combined sources15
Beta strandi72 – 75Combined sources4
Helixi79 – 83Combined sources5
Helixi87 – 91Combined sources5
Turni92 – 94Combined sources3
Helixi97 – 111Combined sources15
Helixi118 – 123Combined sources6
Helixi126 – 133Combined sources8
Helixi141 – 150Combined sources10
Helixi163 – 172Combined sources10
Helixi181 – 187Combined sources7
Helixi189 – 191Combined sources3
Helixi195 – 203Combined sources9
Helixi204 – 207Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B21X-ray1.45A1-213[»]
4B22X-ray1.90A1-213[»]
4B23X-ray2.00A1-213[»]
4B24X-ray2.30A1-213[»]
4HSBX-ray1.90A1-213[»]
ProteinModelPortaliO94468.
SMRiO94468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000261908.
InParanoidiO94468.
OMAiQLCEKWK.
OrthoDBiEOG092C51YL.
PhylomeDBiO94468.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKDSDYKRA EKHLSSIDNK WSSLVKKVGP CTLTPHPEHA PYEGIIRAIT
60 70 80 90 100
SQKLSDAATN SIINKFCTQC SDNDEFPTPK QIMETDVETL HECGFSKLKS
110 120 130 140 150
QEIHIVAEAA LNKQIPSKSE IEKMSEEELM ESLSKIKGVK RWTIEMYSIF
160 170 180 190 200
TLGRLDIMPA DDSTLKNEAK EFFGLSSKPQ TEEVEKLTKP CKPYRTIAAW
210
YLWQIPKLHR KGQ
Length:213
Mass (Da):24,292
Last modified:May 1, 1999 - v1
Checksum:i21E7E34A181D80EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22627.1.
PIRiT39957.
RefSeqiNP_595869.1. NM_001021775.2.

Genome annotation databases

EnsemblFungiiSPBC23G7.11.1; SPBC23G7.11.1:pep; SPBC23G7.11.
GeneIDi2540646.
KEGGispo:SPBC23G7.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22627.1.
PIRiT39957.
RefSeqiNP_595869.1. NM_001021775.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B21X-ray1.45A1-213[»]
4B22X-ray1.90A1-213[»]
4B23X-ray2.00A1-213[»]
4B24X-ray2.30A1-213[»]
4HSBX-ray1.90A1-213[»]
ProteinModelPortaliO94468.
SMRiO94468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277171. 5 interactors.
MINTiMINT-4684434.

Proteomic databases

MaxQBiO94468.
PRIDEiO94468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC23G7.11.1; SPBC23G7.11.1:pep; SPBC23G7.11.
GeneIDi2540646.
KEGGispo:SPBC23G7.11.

Organism-specific databases

EuPathDBiFungiDB:SPBC23G7.11.
PomBaseiSPBC23G7.11. mag2.

Phylogenomic databases

HOGENOMiHOG000261908.
InParanoidiO94468.
OMAiQLCEKWK.
OrthoDBiEOG092C51YL.
PhylomeDBiO94468.

Miscellaneous databases

PROiO94468.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAG2_SCHPO
AccessioniPrimary (citable) accession number: O94468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.