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O94468

- MAG2_SCHPO

UniProt

O94468 - MAG2_SCHPO

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Protein
Probable DNA-3-methyladenine glycosylase 2
Gene
mag2, SPBC23G7.11
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Invlved in base excision repair of methyl methanesulfonate-damaged DNA by hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.1 Publication

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei142 – 1421Determinant for substrate specificity and/or activity By similarity
Active sitei162 – 1621Proton acceptor By similarity

GO - Molecular functioni

  1. DNA-3-methyladenine glycosylase activity Source: UniProtKB-EC
  2. DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
  3. DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
  4. DNA-7-methylguanine glycosylase activity Source: UniProtKB-EC
  5. alkylbase DNA N-glycosylase activity Source: PomBase

GO - Biological processi

  1. DNA dealkylation involved in DNA repair Source: PomBase
  2. base-excision repair, AP site formation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA-3-methyladenine glycosylase 2 (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase 2
3MEA DNA glycosylase 2
Gene namesi
Name:mag2
ORF Names:SPBC23G7.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC23G7.11.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Probable DNA-3-methyladenine glycosylase 2
PRO_0000194882Add
BLAST

Proteomic databases

MaxQBiO94468.

Interactioni

Protein-protein interaction databases

BioGridi277171. 4 interactions.
MINTiMINT-4684434.
STRINGi4896.SPBC23G7.11-1.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412
Turni15 – 173
Helixi19 – 2810
Helixi41 – 5010
Turni51 – 533
Helixi56 – 7015
Beta strandi72 – 754
Helixi79 – 835
Helixi87 – 915
Turni92 – 943
Helixi97 – 11115
Helixi118 – 1236
Helixi126 – 1338
Helixi141 – 15010
Helixi163 – 17210
Helixi181 – 1877
Helixi189 – 1913
Helixi195 – 2039
Helixi204 – 2074

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B21X-ray1.45A1-213[»]
4B22X-ray1.90A1-213[»]
4B23X-ray2.00A1-213[»]
4B24X-ray2.30A1-213[»]
4HSBX-ray1.90A1-213[»]
ProteinModelPortaliO94468.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0122.
HOGENOMiHOG000261908.
KOiK01247.
OMAiCAGHPDI.
OrthoDBiEOG78SQVJ.
PhylomeDBiO94468.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94468-1 [UniParc]FASTAAdd to Basket

« Hide

MSKDSDYKRA EKHLSSIDNK WSSLVKKVGP CTLTPHPEHA PYEGIIRAIT    50
SQKLSDAATN SIINKFCTQC SDNDEFPTPK QIMETDVETL HECGFSKLKS 100
QEIHIVAEAA LNKQIPSKSE IEKMSEEELM ESLSKIKGVK RWTIEMYSIF 150
TLGRLDIMPA DDSTLKNEAK EFFGLSSKPQ TEEVEKLTKP CKPYRTIAAW 200
YLWQIPKLHR KGQ 213
Length:213
Mass (Da):24,292
Last modified:May 1, 1999 - v1
Checksum:i21E7E34A181D80EE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329671 Genomic DNA. Translation: CAA22627.1.
PIRiT39957.
RefSeqiNP_595869.1. NM_001021775.2.

Genome annotation databases

EnsemblFungiiSPBC23G7.11.1; SPBC23G7.11.1:pep; SPBC23G7.11.
GeneIDi2540646.
KEGGispo:SPBC23G7.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329671 Genomic DNA. Translation: CAA22627.1 .
PIRi T39957.
RefSeqi NP_595869.1. NM_001021775.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4B21 X-ray 1.45 A 1-213 [» ]
4B22 X-ray 1.90 A 1-213 [» ]
4B23 X-ray 2.00 A 1-213 [» ]
4B24 X-ray 2.30 A 1-213 [» ]
4HSB X-ray 1.90 A 1-213 [» ]
ProteinModelPortali O94468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277171. 4 interactions.
MINTi MINT-4684434.
STRINGi 4896.SPBC23G7.11-1.

Proteomic databases

MaxQBi O94468.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC23G7.11.1 ; SPBC23G7.11.1:pep ; SPBC23G7.11 .
GeneIDi 2540646.
KEGGi spo:SPBC23G7.11.

Organism-specific databases

PomBasei SPBC23G7.11.

Phylogenomic databases

eggNOGi COG0122.
HOGENOMi HOG000261908.
KOi K01247.
OMAi CAGHPDI.
OrthoDBi EOG78SQVJ.
PhylomeDBi O94468.

Miscellaneous databases

NextBioi 20801770.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProi IPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view ]
Pfami PF00730. HhH-GPD. 1 hit.
[Graphical view ]
SMARTi SM00478. ENDO3c. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
PROSITEi PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  3. "Involvement of 3-methyladenine DNA glycosylases Mag1p and Mag2p in base excision repair of methyl methanesulfonate-damaged DNA in the fission yeast Schizosaccharomyces pombe."
    Kanamitsu K., Tanihigashi H., Tanita Y., Inatani S., Ikeda S.
    Genes Genet. Syst. 82:489-494(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMAG2_SCHPO
AccessioniPrimary (citable) accession number: O94468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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