Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O94468

- MAG2_SCHPO

UniProt

O94468 - MAG2_SCHPO

Protein

Probable DNA-3-methyladenine glycosylase 2

Gene

mag2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Invlved in base excision repair of methyl methanesulfonate-damaged DNA by hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.1 Publication

    Catalytic activityi

    Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei142 – 1421Determinant for substrate specificity and/or activityBy similarity
    Active sitei162 – 1621Proton acceptorBy similarity

    GO - Molecular functioni

    1. alkylbase DNA N-glycosylase activity Source: PomBase
    2. DNA-3-methyladenine glycosylase activity Source: UniProtKB-EC
    3. DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
    4. DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
    5. DNA-7-methylguanine glycosylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. base-excision repair, AP site formation Source: PomBase
    2. DNA dealkylation involved in DNA repair Source: PomBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable DNA-3-methyladenine glycosylase 2 (EC:3.2.2.21)
    Alternative name(s):
    3-methyladenine DNA glycosidase 2
    3MEA DNA glycosylase 2
    Gene namesi
    Name:mag2
    ORF Names:SPBC23G7.11
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC23G7.11.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213Probable DNA-3-methyladenine glycosylase 2PRO_0000194882Add
    BLAST

    Proteomic databases

    MaxQBiO94468.

    Interactioni

    Protein-protein interaction databases

    BioGridi277171. 4 interactions.
    MINTiMINT-4684434.
    STRINGi4896.SPBC23G7.11-1.

    Structurei

    Secondary structure

    1
    213
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1412
    Turni15 – 173
    Helixi19 – 2810
    Helixi41 – 5010
    Turni51 – 533
    Helixi56 – 7015
    Beta strandi72 – 754
    Helixi79 – 835
    Helixi87 – 915
    Turni92 – 943
    Helixi97 – 11115
    Helixi118 – 1236
    Helixi126 – 1338
    Helixi141 – 15010
    Helixi163 – 17210
    Helixi181 – 1877
    Helixi189 – 1913
    Helixi195 – 2039
    Helixi204 – 2074

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4B21X-ray1.45A1-213[»]
    4B22X-ray1.90A1-213[»]
    4B23X-ray2.00A1-213[»]
    4B24X-ray2.30A1-213[»]
    4HSBX-ray1.90A1-213[»]
    ProteinModelPortaliO94468.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0122.
    HOGENOMiHOG000261908.
    KOiK01247.
    OMAiCAGHPDI.
    OrthoDBiEOG78SQVJ.
    PhylomeDBiO94468.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
    IPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR023170. HTH_base_excis_C.
    [Graphical view]
    PfamiPF00730. HhH-GPD. 1 hit.
    [Graphical view]
    SMARTiSM00478. ENDO3c. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94468-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKDSDYKRA EKHLSSIDNK WSSLVKKVGP CTLTPHPEHA PYEGIIRAIT    50
    SQKLSDAATN SIINKFCTQC SDNDEFPTPK QIMETDVETL HECGFSKLKS 100
    QEIHIVAEAA LNKQIPSKSE IEKMSEEELM ESLSKIKGVK RWTIEMYSIF 150
    TLGRLDIMPA DDSTLKNEAK EFFGLSSKPQ TEEVEKLTKP CKPYRTIAAW 200
    YLWQIPKLHR KGQ 213
    Length:213
    Mass (Da):24,292
    Last modified:May 1, 1999 - v1
    Checksum:i21E7E34A181D80EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA22627.1.
    PIRiT39957.
    RefSeqiNP_595869.1. NM_001021775.2.

    Genome annotation databases

    EnsemblFungiiSPBC23G7.11.1; SPBC23G7.11.1:pep; SPBC23G7.11.
    GeneIDi2540646.
    KEGGispo:SPBC23G7.11.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA22627.1 .
    PIRi T39957.
    RefSeqi NP_595869.1. NM_001021775.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4B21 X-ray 1.45 A 1-213 [» ]
    4B22 X-ray 1.90 A 1-213 [» ]
    4B23 X-ray 2.00 A 1-213 [» ]
    4B24 X-ray 2.30 A 1-213 [» ]
    4HSB X-ray 1.90 A 1-213 [» ]
    ProteinModelPortali O94468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277171. 4 interactions.
    MINTi MINT-4684434.
    STRINGi 4896.SPBC23G7.11-1.

    Proteomic databases

    MaxQBi O94468.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC23G7.11.1 ; SPBC23G7.11.1:pep ; SPBC23G7.11 .
    GeneIDi 2540646.
    KEGGi spo:SPBC23G7.11.

    Organism-specific databases

    PomBasei SPBC23G7.11.

    Phylogenomic databases

    eggNOGi COG0122.
    HOGENOMi HOG000261908.
    KOi K01247.
    OMAi CAGHPDI.
    OrthoDBi EOG78SQVJ.
    PhylomeDBi O94468.

    Miscellaneous databases

    NextBioi 20801770.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    InterProi IPR000035. Alkylbase_DNA_glycsylse_CS.
    IPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR023170. HTH_base_excis_C.
    [Graphical view ]
    Pfami PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    SMARTi SM00478. ENDO3c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    PROSITEi PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    3. "Involvement of 3-methyladenine DNA glycosylases Mag1p and Mag2p in base excision repair of methyl methanesulfonate-damaged DNA in the fission yeast Schizosaccharomyces pombe."
      Kanamitsu K., Tanihigashi H., Tanita Y., Inatani S., Ikeda S.
      Genes Genet. Syst. 82:489-494(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMAG2_SCHPO
    AccessioniPrimary (citable) accession number: O94468
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3