O94467 (OYEC_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative NADPH dehydrogenase C23G7.10c
EC=1.6.99.1

Alternative name(s):
Old yellow enzyme homolog 3

Gene names

ORF Names:

SPBC23G7.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor.
Cofactor	FMN By similarity.
Subunit structure	Homodimer or heterodimer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to stress Inferred from expression pattern. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Putative NADPH dehydrogenase C23G7.10c		PRO_0000314118
Sites
	Active site	213	1	Proton donor By similarity
	Binding site	208	1	FMN By similarity
	Binding site	208	1	Substrate By similarity
	Binding site	358	1	FMN By similarity
	Binding site	386	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94467 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: EF4687841D9FDA06	FASTA	395	43,684
	10 20 30 40 50 60 MTIVNEGAEN VGYFTPAQKI PAGAAIGVPQ TKLFTPLKIR GVEFHNRMFV SPMCTYSADQ 70 80 90 100 110 120 EGHLTDFHLV HLGAMGMRGP GLVMVEATAV SPEGRISPND SGLWMESQMK PLRRIVEFAH 130 140 150 160 170 180 SQNQKIGIQL AHAGRKASTT APYRGYTVAT EAQGGWENDV YGPNEDRWDE NHAQPHKLTE 190 200 210 220 230 240 KQYDELVDKF VVAAKRAVEI GFDVIEIHGA HGYLISSTVS PATNDRNDKY GGTFEKRILF 250 260 270 280 290 300 PMEVVHSVRK AIPDSMPLFY RVTATDWLPK GQGWEIEDTV ALAARLRDGG VDLIDVSSGG 310 320 330 340 350 360 NHKDQRIEVK DCYQVPFAEK IKDQVNGILL GAVGMIRDGL TANEILESGK ADVTFVAREF 370 380 390 LRNPSLVLDS ANQLGENVAW PVQYDYAVKG HRKLR

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA22626.1.
PIR	T39956.
RefSeq	NP_595868.1. NM_001021774.1.
3D structure databases
HSSP	HSSP built from PDB template 1Z41 based on UniProtKB P54550.
ProteinModelPortal	O94467.
ModBase	Search...
Protein-protein interaction databases
STRING	O94467.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC23G7.10c.1; SPBC23G7.10c.1:pep; SPBC23G7.10c.
GeneID	2540490.
KEGG	spo:SPBC23G7.10c.
NMPDR	fig|4896.1.peg.1734.
Organism-specific databases
GeneDB_Spombe	SPBC23G7.10c.
Phylogenomic databases
eggNOG	fuNOG07277.
GeneTree	EFGT00050000000722.
HOGENOM	HBG583461.
OMA	MCTYSAD.
OrthoDB	EOG4M68S3.
Gene expression databases
ArrayExpress	O94467.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OYEC_SCHPO

Accession

Primary (citable) accession number: O94467

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 1999
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program