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Protein

Probable queuine tRNA-ribosyltransferase

Gene

SPAC1687.19c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).By similarity

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991SubstrateBy similarity
Active sitei272 – 2721NucleophileBy similarity
Metal bindingi310 – 3101ZincBy similarity
Metal bindingi312 – 3121ZincBy similarity
Metal bindingi315 – 3151ZincBy similarity
Metal bindingi347 – 3471ZincBy similarity

GO - Molecular functioni

GO - Biological processi

  • queuosine biosynthetic process Source: UniProtKB-UniPathway
  • tRNA modification Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable queuine tRNA-ribosyltransferase (EC:2.4.2.29)
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene namesi
ORF Names:SPAC1687.19c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1687.19c.
PomBaseiSPAC1687.19c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Probable queuine tRNA-ribosyltransferasePRO_0000135569Add
BLAST

Proteomic databases

MaxQBiO94460.

Interactioni

Protein-protein interaction databases

BioGridi278609. 3 interactions.
MINTiMINT-4684371.

Structurei

3D structure databases

ProteinModelPortaliO94460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000223473.
InParanoidiO94460.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG7XH70C.
PhylomeDBiO94460.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

O94460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSFPALQF KVVARCSTTR ARVTDIQLPH GLVESPVFMP VGTQASLKGV
60 70 80 90 100
LPEQLDALGC KIMLNNTYHL GLKPGQEVLD TVGGAHRFQS WNKNILTDSG
110 120 130 140 150
GFQMVSLLKL ATITEDGVTF LSPRDGTPML LTPEHSISLQ NSIGSDIMMQ
160 170 180 190 200
LDDVVHTLTE SKRMEEAMYR SIRWLDRCIQ AHKRPETQNL FCIIQGGLDK
210 220 230 240 250
RLREICCREM VKRNTPGIAV GGLSGGEEKH AFCETVYTCT SILPDNKPRY
260 270 280 290 300
LMGVGYAEDL VVCVALGMDM FDCVYPTRTA RFGNALTRKG VINLRNQKFR
310 320 330 340 350
NDIGPLEEGC SCPCCKTELE GGWGITRAYF NSLVSKETVG ANLMTIHNVH
360 370 380 390 400
FQLQLMRDMR ESIIKDEFPS FVKNFFHEWN HGDKSNYPSW AVDALRMVNI

DLLA
Length:404
Mass (Da):45,176
Last modified:May 1, 1999 - v1
Checksum:iD192781D8854D4C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22613.1.
PIRiT37762.
RefSeqiNP_593138.1. NM_001018534.2.

Genome annotation databases

EnsemblFungiiSPAC1687.19c.1; SPAC1687.19c.1:pep; SPAC1687.19c.
GeneIDi2542133.
KEGGispo:SPAC1687.19c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22613.1.
PIRiT37762.
RefSeqiNP_593138.1. NM_001018534.2.

3D structure databases

ProteinModelPortaliO94460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278609. 3 interactions.
MINTiMINT-4684371.

Proteomic databases

MaxQBiO94460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1687.19c.1; SPAC1687.19c.1:pep; SPAC1687.19c.
GeneIDi2542133.
KEGGispo:SPAC1687.19c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1687.19c.
PomBaseiSPAC1687.19c.

Phylogenomic databases

HOGENOMiHOG000223473.
InParanoidiO94460.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG7XH70C.
PhylomeDBiO94460.

Enzyme and pathway databases

UniPathwayiUPA00392.

Miscellaneous databases

NextBioi20803205.
PROiO94460.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiTGT_SCHPO
AccessioniPrimary (citable) accession number: O94460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 1, 1999
Last modified: May 11, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.