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Protein

E3 SUMO-protein ligase pli1

Gene

pli1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an E3 ligase mediating SUMO/Smt3 attachment to other proteins. Involved in the maintenance of the centromere and in telomere length. Regulates recombination, via extension sumoylation, particularly within the heterochromatin repeats.1 Publication

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri290 – 367SP-RING-typePROSITE-ProRule annotationAdd BLAST78

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • SUMO ligase activity Source: PomBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • linear element assembly Source: PomBase
  • negative regulation of double-strand break repair via homologous recombination Source: PomBase
  • protein sumoylation Source: PomBase
  • reciprocal meiotic recombination Source: PomBase
  • regulation of mitotic recombination Source: PomBase
  • regulation of reciprocal meiotic recombination Source: PomBase
  • regulation of telomere maintenance via recombination Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-3232118. SUMOylation of transcription factors.
R-SPO-4615885. SUMOylation of DNA replication proteins.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase pli1 (EC:6.3.2.-)
Gene namesi
Name:pli1
ORF Names:SPAC1687.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1687.05.
PomBaseiSPAC1687.05. pli1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi321C → S: Reduction in sumoylation of rad22. 1 Publication1
Mutagenesisi323H → A: Reduction in sumoylation of rad22. 1 Publication1
Mutagenesisi326C → S: Reduction in sumoylation of rad22. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189861 – 727E3 SUMO-protein ligase pli1Add BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395Phosphoserine1 Publication1
Modified residuei396Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94451.
PRIDEiO94451.

PTM databases

iPTMnetiO94451.
SwissPalmiO94451.

Interactioni

Subunit structurei

Interacts with hus5/ubc9.1 Publication

Protein-protein interaction databases

BioGridi278077. 87 interactors.
DIPiDIP-38715N.
IntActiO94451. 2 interactors.
MINTiMINT-4684285.

Structurei

3D structure databases

ProteinModelPortaliO94451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 52SAPPROSITE-ProRule annotationAdd BLAST35
Domaini108 – 261PINITPROSITE-ProRule annotationAdd BLAST154

Sequence similaritiesi

Belongs to the PIAS family.Curated
Contains 1 PINIT domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation
Contains 1 SP-RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri290 – 367SP-RING-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiO94451.
KOiK04706.
OMAiPAKKVAY.
OrthoDBiEOG092C445R.
PhylomeDBiO94451.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR023321. PINIT.
IPR003034. SAP_dom.
IPR027229. SIZ1/SIZ2/Pli1/Gei17.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10782:SF4. PTHR10782:SF4. 1 hit.
PfamiPF14324. PINIT. 1 hit.
PF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQANFLQEL PNVLKRLETG LIIPQLKDIL RVFGLRLSGT KAELITRIKQ
60 70 80 90 100
LIERIAIENN TTSWEALKKA IDGDVTSAVC ILKYNTYQIY SAAAPIAPPS
110 120 130 140 150
SASGNRSYSR PFAPVVHSRI RFRKSPFYDI LEQFNAPFVV PACVGTRNTI
160 170 180 190 200
SFSFHVTPPA LSKLLNDPKQ YRVYLFSTPS ETIGFGNCLM EFPTPQMELR
210 220 230 240 250
INNQVAHANY RRLKGKPGTT NPADITDLVS KYAGPPGNNV VIYYMNSTKS
260 270 280 290 300
YSVVVCFVKV YTIENLVDQI KSRKAESKEK IIERIKNDNQ DADIIATSTD
310 320 330 340 350
ISLKCPLSFS RISLPVRSVF CKHIQCFDAS AFLEMNKQTP SWMCPVCASH
360 370 380 390 400
IQFSDLIIDG FMQHILESTP SNSETITVDP EGNWKLNTFD EPVESSEDEF
410 420 430 440 450
VPKEKVIELS DGEGISTMAN KSNDQPTRRA STHNSGPPAK RKRESLVIDL
460 470 480 490 500
TISDDDENVA TSTTESPSNA TKENSLSRNV QSPNIDTAIS NRSTNVRHGH
510 520 530 540 550
PGFKDYTVEN SPASRERSTS ESAQSSVHMG YAGEGGLLSG ALRAPSQQNN
560 570 580 590 600
NNSNTQHSIN LHTIVPSPYE PPLSVTPSTA ITNLSIPESN RTNSSASSKS
610 620 630 640 650
FTMNDLILPP LHLKNTTQTN NAHEDAQSSN LSQNHSLFYE RIPQRPSYRI
660 670 680 690 700
EKQNKGIYED ENEQSISAMP IPRAHPQLPK NLLSQTAGPL WDEQQDAQVD
710 720
WNSELQSNNS YHNSGFEGTG NTFQSID
Length:727
Mass (Da):80,723
Last modified:February 1, 2005 - v3
Checksum:i56228A04888DD235
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22599.1.
AB027790 Genomic DNA. Translation: BAA87094.1.
PIRiT37748.
RefSeqiNP_593123.1. NM_001018519.2.

Genome annotation databases

EnsemblFungiiSPAC1687.05.1; SPAC1687.05.1:pep; SPAC1687.05.
GeneIDi2541580.
KEGGispo:SPAC1687.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22599.1.
AB027790 Genomic DNA. Translation: BAA87094.1.
PIRiT37748.
RefSeqiNP_593123.1. NM_001018519.2.

3D structure databases

ProteinModelPortaliO94451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278077. 87 interactors.
DIPiDIP-38715N.
IntActiO94451. 2 interactors.
MINTiMINT-4684285.

PTM databases

iPTMnetiO94451.
SwissPalmiO94451.

Proteomic databases

MaxQBiO94451.
PRIDEiO94451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1687.05.1; SPAC1687.05.1:pep; SPAC1687.05.
GeneIDi2541580.
KEGGispo:SPAC1687.05.

Organism-specific databases

EuPathDBiFungiDB:SPAC1687.05.
PomBaseiSPAC1687.05. pli1.

Phylogenomic databases

InParanoidiO94451.
KOiK04706.
OMAiPAKKVAY.
OrthoDBiEOG092C445R.
PhylomeDBiO94451.

Enzyme and pathway databases

UniPathwayiUPA00886.
ReactomeiR-SPO-3232118. SUMOylation of transcription factors.
R-SPO-4615885. SUMOylation of DNA replication proteins.

Miscellaneous databases

PROiO94451.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR023321. PINIT.
IPR003034. SAP_dom.
IPR027229. SIZ1/SIZ2/Pli1/Gei17.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10782:SF4. PTHR10782:SF4. 1 hit.
PfamiPF14324. PINIT. 1 hit.
PF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLI1_SCHPO
AccessioniPrimary (citable) accession number: O94451
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: October 5, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.