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Protein

Replication factor C subunit 4

Gene

rfc4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241ATP; via carbonyl oxygenBy similarity
Binding sitei36 – 361ATP; via amide nitrogenBy similarity
Binding sitei157 – 1571ATPBy similarity
Binding sitei215 – 2151ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 688ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA strand elongation involved in DNA replication Source: PomBase
  • mitotic DNA replication leading strand elongation Source: PomBase
  • UV-damage excision repair Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-110320. Translesion Synthesis by POLH.
R-SPO-174411. Polymerase switching on the C-strand of the telomere.
R-SPO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.
R-SPO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-SPO-5696400. Dual Incision in GG-NER.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-69091. Polymerase switching.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 4
Short name:
Replication factor C4
Gene namesi
Name:rfc4
ORF Names:SPAC1687.03c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1687.03c.
PomBaseiSPAC1687.03c. rfc4.

Subcellular locationi

GO - Cellular componenti

  • Ctf18 RFC-like complex Source: PomBase
  • cytosol Source: PomBase
  • Elg1 RFC-like complex Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear DNA replication factor C complex Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
  • Rad17 RFC-like complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Replication factor C subunit 4PRO_0000121770Add
BLAST

Proteomic databases

MaxQBiO94449.

Interactioni

Subunit structurei

Heteropentamer of subunits rfc1, rfc2, rfc3, rfc4 and rfc5 that forms a complex (RFC) with PCNA in the presence of ATP. Two other complexes exist where rfc1 can be replaced by either ctf18 or elg1 to form the ctf18-RFC or the elg1-RFC complexes respectively.1 Publication

Protein-protein interaction databases

BioGridi279239. 6 interactions.
MINTiMINT-4684262.

Structurei

3D structure databases

ProteinModelPortaliO94449.
SMRiO94449. Positions 20-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

HOGENOMiHOG000224155.
InParanoidiO94449.
KOiK10755.
OMAiYTNDGLE.
OrthoDBiEOG7DZ8VQ.
PhylomeDBiO94449.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

O94449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNAVSSSVF GEKNNSVAYE LPWVEKYRPI VLDDIVGNEE TIDRLKVIAK
60 70 80 90 100
EGNMPHLVIS GMPGIGKTTS ILCLAHALLG PAYKEGVLEL NASDERGIDV
110 120 130 140 150
VRNRIKAFAQ KKVILPPGRH KIIILDEADS MTAGAQQALR RTMEIYSNTT
160 170 180 190 200
RFALACNQSN KIIEPIQSRC AILRYSRLTD QQVLQRLLNI CKAEKVNYTD
210 220 230 240 250
DGLAALIMTA EGDMRQAVNN LQSTVAGFGL VNGENVFRVA DQPSPVAIHA
260 270 280 290 300
MLTACQSGNI DVALEKLQGI WDLGFSAVDI VTNMFRVVKT MDSIPEFSRL
310 320 330 340
EMLKEIGQTH MIILEGVQTL LQLSGLVCRL AKSQMKPESF II
Length:342
Mass (Da):37,655
Last modified:May 1, 1999 - v1
Checksum:iBD3504BC2E2F60ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22597.1.
PIRiT37746.
RefSeqiNP_593121.1. NM_001018517.2.

Genome annotation databases

EnsemblFungiiSPAC1687.03c.1; SPAC1687.03c.1:pep; SPAC1687.03c.
GeneIDi2542790.
KEGGispo:SPAC1687.03c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22597.1.
PIRiT37746.
RefSeqiNP_593121.1. NM_001018517.2.

3D structure databases

ProteinModelPortaliO94449.
SMRiO94449. Positions 20-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279239. 6 interactions.
MINTiMINT-4684262.

Proteomic databases

MaxQBiO94449.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1687.03c.1; SPAC1687.03c.1:pep; SPAC1687.03c.
GeneIDi2542790.
KEGGispo:SPAC1687.03c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1687.03c.
PomBaseiSPAC1687.03c. rfc4.

Phylogenomic databases

HOGENOMiHOG000224155.
InParanoidiO94449.
KOiK10755.
OMAiYTNDGLE.
OrthoDBiEOG7DZ8VQ.
PhylomeDBiO94449.

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-110320. Translesion Synthesis by POLH.
R-SPO-174411. Polymerase switching on the C-strand of the telomere.
R-SPO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.
R-SPO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-SPO-5696400. Dual Incision in GG-NER.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-69091. Polymerase switching.

Miscellaneous databases

PROiO94449.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast."
    Kim J., Robertson K., Mylonas K.J.L., Gray F.C., Charapitsa I., MacNeill S.A.
    Nucleic Acids Res. 33:4078-4089(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 14-46; 51-67; 85-96; 112-119; 122-150; 162-174; 178-186; 196-238; 267-286; 291-299 AND 333-342, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiRFC4_SCHPO
AccessioniPrimary (citable) accession number: O94449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.