Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

26S proteasome regulatory subunit rpn10

Gene

rpn10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protects ubiquitin chains against dissambly by deubiquitinating enzymes thereby promoting protein degradation.2 Publications

GO - Molecular functioni

  • K48-linked polyubiquitin binding Source: PomBase

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-5689603. UCH proteinases.
R-SPO-5689880. Ub-specific processing proteases.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit rpn10
Gene namesi
Name:rpn10
Synonyms:pus1
ORF Names:SPAC637.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC637.10c.
PomBaseiSPAC637.10c. rpn10.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
  • proteasome regulatory particle, base subcomplex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738331 – 24326S proteasome regulatory subunit rpn10Add BLAST243

Proteomic databases

MaxQBiO94444.
PRIDEiO94444.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity). Interacts with uch2.By similarity1 Publication

GO - Molecular functioni

  • K48-linked polyubiquitin binding Source: PomBase

Protein-protein interaction databases

BioGridi280096. 22 interactors.
IntActiO94444. 1 interactor.
MINTiMINT-4684209.

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Helixi14 – 17Combined sources4
Beta strandi21 – 23Combined sources3
Helixi25 – 43Combined sources19
Beta strandi48 – 53Combined sources6
Beta strandi61 – 67Combined sources7
Helixi69 – 76Combined sources8
Helixi87 – 99Combined sources13
Beta strandi106 – 114Combined sources9
Helixi122 – 134Combined sources13
Beta strandi137 – 145Combined sources9
Helixi152 – 160Combined sources9
Beta strandi167 – 171Combined sources5
Helixi178 – 183Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X5NX-ray1.30A2-193[»]
ProteinModelPortaliO94444.
SMRiO94444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 185VWFAPROSITE-ProRule annotationAdd BLAST181
Domaini205 – 224UIMPROSITE-ProRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000165630.
InParanoidiO94444.
KOiK03029.
OMAiISMANNC.
OrthoDBiEOG092C5246.
PhylomeDBiO94444.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLEATMILI DNSEWMINGD YIPTRFEAQK DTVHMIFNQK INDNPENMCG
60 70 80 90 100
LMTIGDNSPQ VLSTLTRDYG KFLSAMHDLP VRGNAKFGDG IQIAQLALKH
110 120 130 140 150
RENKIQRQRI VAFVGSPIVE DEKNLIRLAK RMKKNNVAID IIHIGELQNE
160 170 180 190 200
SALQHFIDAA NSSDSCHLVS IPPSPQLLSD LVNQSPIGQG VVASQNQFEY
210 220 230 240
GVDPNLDVEL ALALELSMAE ERARQEVAAQ KSSEETEDKK MQE
Length:243
Mass (Da):27,140
Last modified:May 1, 1999 - v1
Checksum:i9ED58AE82687F79E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22589.1.
PIRiT39002.
RefSeqiNP_594628.1. NM_001020056.2.

Genome annotation databases

EnsemblFungiiSPAC637.10c.1; SPAC637.10c.1:pep; SPAC637.10c.
GeneIDi2543682.
KEGGispo:SPAC637.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22589.1.
PIRiT39002.
RefSeqiNP_594628.1. NM_001020056.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X5NX-ray1.30A2-193[»]
ProteinModelPortaliO94444.
SMRiO94444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280096. 22 interactors.
IntActiO94444. 1 interactor.
MINTiMINT-4684209.

Proteomic databases

MaxQBiO94444.
PRIDEiO94444.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC637.10c.1; SPAC637.10c.1:pep; SPAC637.10c.
GeneIDi2543682.
KEGGispo:SPAC637.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC637.10c.
PomBaseiSPAC637.10c. rpn10.

Phylogenomic databases

HOGENOMiHOG000165630.
InParanoidiO94444.
KOiK03029.
OMAiISMANNC.
OrthoDBiEOG092C5246.
PhylomeDBiO94444.

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-5689603. UCH proteinases.
R-SPO-5689880. Ub-specific processing proteases.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO94444.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPN10_SCHPO
AccessioniPrimary (citable) accession number: O94444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.