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Protein

26S proteasome regulatory subunit rpn10

Gene

rpn10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protects ubiquitin chains against dissambly by deubiquitinating enzymes thereby promoting protein degradation.2 Publications

GO - Molecular functioni

  • K48-linked polyubiquitin binding Source: PomBase

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit rpn10
Gene namesi
Name:rpn10
Synonyms:pus1
ORF Names:SPAC637.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC637.10c.
PomBaseiSPAC637.10c. rpn10.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
  • proteasome regulatory particle, base subcomplex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24324326S proteasome regulatory subunit rpn10PRO_0000173833Add
BLAST

Proteomic databases

MaxQBiO94444.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity). Interacts with uch2.By similarity1 Publication

GO - Molecular functioni

  • K48-linked polyubiquitin binding Source: PomBase

Protein-protein interaction databases

BioGridi280096. 22 interactions.
IntActiO94444. 1 interaction.
MINTiMINT-4684209.

Structurei

Secondary structure

1
243
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Helixi14 – 174Combined sources
Beta strandi21 – 233Combined sources
Helixi25 – 4319Combined sources
Beta strandi48 – 536Combined sources
Beta strandi61 – 677Combined sources
Helixi69 – 768Combined sources
Helixi87 – 9913Combined sources
Beta strandi106 – 1149Combined sources
Helixi122 – 13413Combined sources
Beta strandi137 – 1459Combined sources
Helixi152 – 1609Combined sources
Beta strandi167 – 1715Combined sources
Helixi178 – 1836Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X5NX-ray1.30A2-193[»]
ProteinModelPortaliO94444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 185181VWFAPROSITE-ProRule annotationAdd
BLAST
Domaini205 – 22420UIMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000165630.
InParanoidiO94444.
KOiK03029.
OMAiATSPILM.
OrthoDBiEOG7VDZ15.
PhylomeDBiO94444.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLEATMILI DNSEWMINGD YIPTRFEAQK DTVHMIFNQK INDNPENMCG
60 70 80 90 100
LMTIGDNSPQ VLSTLTRDYG KFLSAMHDLP VRGNAKFGDG IQIAQLALKH
110 120 130 140 150
RENKIQRQRI VAFVGSPIVE DEKNLIRLAK RMKKNNVAID IIHIGELQNE
160 170 180 190 200
SALQHFIDAA NSSDSCHLVS IPPSPQLLSD LVNQSPIGQG VVASQNQFEY
210 220 230 240
GVDPNLDVEL ALALELSMAE ERARQEVAAQ KSSEETEDKK MQE
Length:243
Mass (Da):27,140
Last modified:May 1, 1999 - v1
Checksum:i9ED58AE82687F79E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22589.1.
PIRiT39002.
RefSeqiNP_594628.1. NM_001020056.2.

Genome annotation databases

EnsemblFungiiSPAC637.10c.1; SPAC637.10c.1:pep; SPAC637.10c.
GeneIDi2543682.
KEGGispo:SPAC637.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22589.1.
PIRiT39002.
RefSeqiNP_594628.1. NM_001020056.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X5NX-ray1.30A2-193[»]
ProteinModelPortaliO94444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280096. 22 interactions.
IntActiO94444. 1 interaction.
MINTiMINT-4684209.

Proteomic databases

MaxQBiO94444.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC637.10c.1; SPAC637.10c.1:pep; SPAC637.10c.
GeneIDi2543682.
KEGGispo:SPAC637.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC637.10c.
PomBaseiSPAC637.10c. rpn10.

Phylogenomic databases

HOGENOMiHOG000165630.
InParanoidiO94444.
KOiK03029.
OMAiATSPILM.
OrthoDBiEOG7VDZ15.
PhylomeDBiO94444.

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO94444.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromised."
    Wilkinson C.R.M., Ferrell K., Penney M., Wallace M., Dubiel W., Gordon C.
    J. Biol. Chem. 275:15182-15192(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Ubiquitin binding proteins protect ubiquitin conjugates from disassembly."
    Hartmann-Petersen R., Hendil K.B., Gordon C.
    FEBS Lett. 535:77-81(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast."
    Stone M., Hartmann-Petersen R., Seeger M., Bech-Otschir D., Wallace M., Gordon C.
    J. Mol. Biol. 344:697-706(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UCH2.

Entry informationi

Entry nameiRPN10_SCHPO
AccessioniPrimary (citable) accession number: O94444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.