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Protein

Cytosolic Fe-S cluster assembly factor nbp35

Gene

nbp35

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The nbp35-cfd1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of nbp35 and two labile, bridging clusters between subunits of the nbp35-cfd1 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi15 – 151Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi29 – 291Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi32 – 321Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi38 – 381Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi241 – 2411Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi244 – 2441Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 758ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor nbp35UniRule annotation
Alternative name(s):
Nucleotide-binding protein 35UniRule annotation
Gene namesi
Name:nbp35
ORF Names:SPAC637.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC637.08.
PomBaseiSPAC637.08. nbp35.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Cytosolic Fe-S cluster assembly factor nbp35PRO_0000278902Add
BLAST

Proteomic databases

MaxQBiO94442.

Interactioni

Subunit structurei

Heterotetramer of 2 nbp35 and 2 cfd1 chains.UniRule annotation

Protein-protein interaction databases

MINTiMINT-4684190.

Structurei

3D structure databases

ProteinModelPortaliO94442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000079916.
InParanoidiO94442.
OMAiQRIREFC.
OrthoDBiEOG092C4BLN.
PhylomeDBiO94442.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIQETVPLD APEHCPGPSS ENAGTASACE GCPNQQICAS APRGEDPDLP
60 70 80 90 100
LVVERLKEIK HKILVLSGKG GVGKSTFSSQ LAWGLSLEED KQIGLMDVDI
110 120 130 140 150
CGPSIPRIMG VEKEEAHQSS KGWSPIYVCP NLAVMSIGFL LPSEDSSVIW
160 170 180 190 200
RGPKKNGLIK QFIKDVNWEN LDYLIVDTPP GTSDEHLSLV QFFKNSGIDG
210 220 230 240 250
AVVVTTPQEV ALQDVRKEID FCRKASIPIL GLVENMSGFV CPSCKGKSNI
260 270 280 290 300
FTITTGGGEA LAKEMGLPFL GKIPLDPLIA RSCDFGKSFI DECPESPASE
310
IIIDIINKID ESLQKRQ
Length:317
Mass (Da):34,261
Last modified:May 1, 1999 - v1
Checksum:i44CBB7D4633AAA41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22587.1.
PIRiT39000.
RefSeqiNP_594626.1. NM_001020054.2.

Genome annotation databases

EnsemblFungiiSPAC637.08.1; SPAC637.08.1:pep; SPAC637.08.
GeneIDi2543416.
KEGGispo:SPAC637.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA22587.1.
PIRiT39000.
RefSeqiNP_594626.1. NM_001020054.2.

3D structure databases

ProteinModelPortaliO94442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4684190.

Proteomic databases

MaxQBiO94442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC637.08.1; SPAC637.08.1:pep; SPAC637.08.
GeneIDi2543416.
KEGGispo:SPAC637.08.

Organism-specific databases

EuPathDBiFungiDB:SPAC637.08.
PomBaseiSPAC637.08. nbp35.

Phylogenomic databases

HOGENOMiHOG000079916.
InParanoidiO94442.
OMAiQRIREFC.
OrthoDBiEOG092C4BLN.
PhylomeDBiO94442.

Miscellaneous databases

PROiO94442.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNBP35_SCHPO
AccessioniPrimary (citable) accession number: O94442
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.