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Protein

Cytosolic Fe-S cluster assembly factor nbp35

Gene

nbp35

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The nbp35-cfd1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of nbp35 and two labile, bridging clusters between subunits of the nbp35-cfd1 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi29Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi32Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi38Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi241Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi244Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 75ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor nbp35UniRule annotation
Alternative name(s):
Nucleotide-binding protein 35UniRule annotation
Gene namesi
Name:nbp35
ORF Names:SPAC637.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC637.08
PomBaseiSPAC637.08 nbp35

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002789021 – 317Cytosolic Fe-S cluster assembly factor nbp35Add BLAST317

Proteomic databases

MaxQBiO94442
PaxDbiO94442
PRIDEiO94442

Interactioni

Subunit structurei

Heterotetramer of 2 nbp35 and 2 cfd1 chains.UniRule annotation

Protein-protein interaction databases

STRINGi4896.SPAC637.08.1

Structurei

3D structure databases

ProteinModelPortaliO94442
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000079916
InParanoidiO94442
OMAiCPNQAIC
OrthoDBiEOG092C4BLN
PhylomeDBiO94442

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03038 NUBP1, 1 hit
InterProiView protein in InterPro
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028601 NUBP1/Nbp35
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit

Sequencei

Sequence statusi: Complete.

O94442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIQETVPLD APEHCPGPSS ENAGTASACE GCPNQQICAS APRGEDPDLP
60 70 80 90 100
LVVERLKEIK HKILVLSGKG GVGKSTFSSQ LAWGLSLEED KQIGLMDVDI
110 120 130 140 150
CGPSIPRIMG VEKEEAHQSS KGWSPIYVCP NLAVMSIGFL LPSEDSSVIW
160 170 180 190 200
RGPKKNGLIK QFIKDVNWEN LDYLIVDTPP GTSDEHLSLV QFFKNSGIDG
210 220 230 240 250
AVVVTTPQEV ALQDVRKEID FCRKASIPIL GLVENMSGFV CPSCKGKSNI
260 270 280 290 300
FTITTGGGEA LAKEMGLPFL GKIPLDPLIA RSCDFGKSFI DECPESPASE
310
IIIDIINKID ESLQKRQ
Length:317
Mass (Da):34,261
Last modified:May 1, 1999 - v1
Checksum:i44CBB7D4633AAA41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA22587.1
PIRiT39000
RefSeqiNP_594626.1, NM_001020054.2

Genome annotation databases

EnsemblFungiiSPAC637.08.1; SPAC637.08.1:pep; SPAC637.08
GeneIDi2543416
KEGGispo:SPAC637.08

Similar proteinsi

Entry informationi

Entry nameiNBP35_SCHPO
AccessioniPrimary (citable) accession number: O94442
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health