ID   MED21_SCHPO             Reviewed;         138 AA.
AC   O94376;
DT   06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   24-JAN-2024, entry version 132.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 21;
DE   AltName: Full=Mediator complex subunit 21;
DE   AltName: Full=Suppressor of RNA polymerase B 7;
GN   Name=med21; Synonyms=srb7; ORFNames=SPBC1604.10;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX.
RX   PubMed=11572939; DOI=10.1073/pnas.211253898;
RA   Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA   Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT   "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT   subunits conserved between yeast and metazoan cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors.
CC   -!- SUBUNIT: Component of the Mediator complex.
CC       {ECO:0000269|PubMed:11572939}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 21 family.
CC       {ECO:0000305}.
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DR   EMBL; CU329671; CAA22343.1; -; Genomic_DNA.
DR   PIR; T39504; T39504.
DR   RefSeq; NP_596630.1; NM_001022551.2.
DR   PDB; 5N9J; X-ray; 3.40 A; D=1-138.
DR   PDB; 5U0P; EM; 4.40 A; U=1-138.
DR   PDB; 5U0S; EM; 7.80 A; U=1-138.
DR   PDBsum; 5N9J; -.
DR   PDBsum; 5U0P; -.
DR   PDBsum; 5U0S; -.
DR   AlphaFoldDB; O94376; -.
DR   EMDB; EMD-8479; -.
DR   EMDB; EMD-8480; -.
DR   SMR; O94376; -.
DR   BioGRID; 276742; 10.
DR   IntAct; O94376; 1.
DR   STRING; 284812.O94376; -.
DR   MaxQB; O94376; -.
DR   PaxDb; 4896-SPBC1604-10-1; -.
DR   EnsemblFungi; SPBC1604.10.1; SPBC1604.10.1:pep; SPBC1604.10.
DR   GeneID; 2540209; -.
DR   KEGG; spo:SPBC1604.10; -.
DR   PomBase; SPBC1604.10; -.
DR   VEuPathDB; FungiDB:SPBC1604.10; -.
DR   eggNOG; KOG1510; Eukaryota.
DR   HOGENOM; CLU_094271_1_1_1; -.
DR   InParanoid; O94376; -.
DR   OMA; LTTYHDH; -.
DR   PhylomeDB; O94376; -.
DR   PRO; PR:O94376; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0060261; P:positive regulation of transcription initiation by RNA polymerase II; EXP:PomBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 6.10.280.10; Mediator complex, subunit Med21; 1.
DR   InterPro; IPR037212; Med7/Med21-like.
DR   InterPro; IPR021384; Mediator_Med21.
DR   PANTHER; PTHR13381:SF0; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21; 1.
DR   PANTHER; PTHR13381; RNA POLYMERASE II HOLOENZYME COMPONENT SRB7; 1.
DR   Pfam; PF11221; Med21; 1.
DR   SUPFAM; SSF140718; Mediator hinge subcomplex-like; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Coiled coil; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..138
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   21"
FT                   /id="PRO_0000096376"
FT   COILED          87..128
FT                   /evidence="ECO:0000255"
FT   HELIX           4..28
FT                   /evidence="ECO:0007829|PDB:5N9J"
FT   STRAND          35..37
FT                   /evidence="ECO:0007829|PDB:5N9J"
FT   HELIX           43..46
FT                   /evidence="ECO:0007829|PDB:5N9J"
FT   HELIX           51..78
FT                   /evidence="ECO:0007829|PDB:5N9J"
FT   HELIX           87..136
FT                   /evidence="ECO:0007829|PDB:5N9J"
SQ   SEQUENCE   138 AA;  15800 MW;  5A7C236108C09AF9 CRC64;
     MACRCTQLQD TIDEVATQFY SSIHYLSSHH DFVPLPGQEK VSDSKVNPIS AEELQFAQRD
     LAKDLVTKFM QIDTLINQLP GISTAPKHQL EKIKKLQNSI EEKQLERKSL ESENEDLKLQ
     LAKRIETFGR LSCVLFQP
//