O94376 (MED21_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 21

Alternative name(s):
Mediator complex subunit 21
Suppressor of RNA polymerase B 7

Gene names

Name:	med21
Synonyms:	srb7
ORF Names:	SPBC1604.10

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Subunit structure	Component of the Mediator complex. Ref.2
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the Mediator complex subunit 21 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase mediator complex Inferred from direct assay PubMed 10625684. Source: PomBase
Molecular_function	RNA polymerase II transcription cofactor activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Mediator of RNA polymerase II transcription subunit 21		PRO_0000096376
Regions
	Coiled coil	87 – 128	42	Potential

Sequences

	Sequence		Length	Mass (Da)
	O94376 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 5A7C236108C09AF9	FASTA	138	15,800
	10 20 30 40 50 60 MACRCTQLQD TIDEVATQFY SSIHYLSSHH DFVPLPGQEK VSDSKVNPIS AEELQFAQRD 70 80 90 100 110 120 LAKDLVTKFM QIDTLINQLP GISTAPKHQL EKIKKLQNSI EEKQLERKSL ESENEDLKLQ 130 LAKRIETFGR LSCVLFQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells." Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D., Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M. Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA22343.1.
PIR	T39504.
RefSeq	NP_596630.1. NM_001022551.2.
3D structure databases
ProteinModelPortal	O94376.
ModBase	Search...
Protein-protein interaction databases
IntAct	O94376. 1 interaction.
STRING	4896.SPBC1604.10-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC1604.10.1; SPBC1604.10.1:pep; SPBC1604.10.
GeneID	2540209.
KEGG	spo:SPBC1604.10.
Organism-specific databases
PomBase	SPBC1604.10.
Phylogenomic databases
eggNOG	NOG247021.
HOGENOM	HOG000001051.
KO	K15152.
OMA	DEVATQF.
OrthoDB	EOG4J9R8J.
Family and domain databases
InterPro	IPR021384. Mediator_Med21. [Graphical view]
Pfam	PF11221. Med21. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801341.

Entry information

Entry name

MED21_SCHPO

Accession

Primary (citable) accession number: O94376

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program