O94374 (IMA2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Importin subunit alpha-2

Alternative name(s):
Importin-1
Karyopherin subunit alpha-2

Gene names

Name:	imp1
ORF Names:	SPBC1604.08c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope. Involved in nuclear protein import. Required for efficient nuclera import of both an SV40 nuclear localization signal-containing reporter protein and the pap1 component of the stress response MAP kinase pathway. Required for proper mitotic progression. Ref.2 Ref.3 Ref.4
Subunit structure	Interacts with pap1. Ref.4
Subcellular location	Cytoplasm. Nucleus › nucleolus. Note: Nucleus; nucleolus; nuclear rim. Ref.3 Ref.4 Ref.5
Sequence similarities	Belongs to the importin alpha family. Contains 8 ARM repeats. Contains 1 IBB domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Protein transport Transport
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	M phase of mitotic cell cycle Inferred from mutant phenotype Ref.3. Source: PomBase NLS-bearing substrate import into nucleus Inferred from direct assay Ref.4. Source: PomBase cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular response to stress Inferred from genetic interaction Ref.4. Source: PomBase establishment of nucleus localization Inferred from mutant phenotype Ref.3. Source: PomBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay Ref.5. Source: PomBase nuclear pore Non-traceable author statement. Source: PomBase nucleolus Inferred from direct assay Ref.2. Source: PomBase
Molecular_function	GTP binding Non-traceable author statement. Source: PomBase protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Importin subunit alpha-2		PRO_0000290665
Regions
	Domain	1 – 57	57	IBB
	Repeat	110 – 149	40	ARM 1
	Repeat	152 – 191	40	ARM 2
	Repeat	194 – 235	42	ARM 3
	Repeat	238 – 277	40	ARM 4
	Repeat	280 – 319	40	ARM 5
	Repeat	322 – 361	40	ARM 6
	Repeat	364 – 403	40	ARM 7
	Repeat	409 – 448	40	ARM 8

Sequences

	Sequence		Length	Mass (Da)
	O94374 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: FFD4919E936E0941	FASTA	539	59,853
	10 20 30 40 50 60 MESRYLSDRR SRFKSKGVFK ADELRRQREE QQIEIRKQKR EESLNKRRNL NAVLQNDIDV 70 80 90 100 110 120 EEEADQSQVQ MEQQMKDEFP KLTADVMSDD IELQLGAVTK FRKYLSKETH PPIDQVIACG 130 140 150 160 170 180 VVDRFVQFLE SEHHLLQFEA AWALTNIASG TTDQTRIVVD SGAVPRFIQL LSSPEKDVRE 190 200 210 220 230 240 QVVWALGNIA GDSSACRDYV LGNGVLQPLL NILQSSASDV SMLRNATWTL SNLCRGKNPP 250 260 270 280 290 300 PNWSTISVAV PILAKLLYSE DVEIIVDACW AISYLSDGPN EKIGAILDVG CAPRLVELLS 310 320 330 340 350 360 SPSVNIQTPA LRSVGNIVTG TDAQTQIIID CGALNAFPSL LSHQKENIRK EACWTISNIT 370 380 390 400 410 420 AGNTQQIQAI IESNLIPPLV HLLSYADYKT KKEACWAISN ATSGGLGQPD QIRYLVSQGV 430 440 450 460 470 480 IKPLCDMLNG SDNKIIQVAL DAIENILKVG EMDRTMDLEN INQYAVYVEE AGGMDMIHDL 490 500 510 520 530 QSSGNNDIYL KAYSIIEKYF SDEDAVEDLA PETENGAFTF GNGPQAQGEF KFDSQDMAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Genome-wide search of Schizosaccharomyces pombe genes causing overexpression-mediated cell cycle defects." Tallada V.A., Daga R.R., Palomeque C., Garzon A., Jimenez J. Yeast 19:1139-1151(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: 972 / ATCC 24843.
[3]	"Identification of genes encoding putative nucleoporins and transport factors in the fission yeast Schizosaccharomyces pombe: a deletion analysis." Chen X.Q., Du X., Liu J., Balasubramanian M.K., Balasundaram D. Yeast 21:495-509(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"The fission yeast Schizosaccharomyces pombe has two importin-alpha proteins, Imp1p and Cut15p, which have common and unique functions in nucleocytoplasmic transport and cell cycle progression." Umeda M., Izaddoost S., Cushman I., Moore M.S., Sazer S. Genetics 171:7-21(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAP1, SUBCELLULAR LOCATION.
[5]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA22341.1.
PIR	T39506.
RefSeq	NP_596632.1. NM_001022553.2.
3D structure databases
ProteinModelPortal	O94374.
SMR	O94374. Positions 78-512.
ModBase	Search...
Protein-protein interaction databases
IntAct	O94374. 1 interaction.
MINT	MINT-4683591.
STRING	4896.SPBC1604.08c-1.
Proteomic databases
PaxDb	O94374.
PRIDE	O94374.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC1604.08c.1; SPBC1604.08c.1:pep; SPBC1604.08c.
GeneID	2539928.
KEGG	spo:SPBC1604.08c.
Organism-specific databases
PomBase	SPBC1604.08c.
Phylogenomic databases
eggNOG	COG5064.
HOGENOM	HOG000167616.
OMA	EMIANIQ.
OrthoDB	EOG408RH7.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR002652. Importin-a_IBB. IPR024931. Importing_su_alpha. [Graphical view]
Pfam	PF00514. Arm. 8 hits. PF01749. IBB. 1 hit. [Graphical view]
PIRSF	PIRSF005673. Importin_alpha. 1 hit.
SMART	SM00185. ARM. 8 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. 4 hits. PS51214. IBB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801071.

Entry information

Entry name

IMA2_SCHPO

Accession

Primary (citable) accession number: O94374

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	May 1, 1999
Last modified:	May 29, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program