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O94354

- ASSY_SCHPO

UniProt

O94354 - ASSY_SCHPO

Protein

Argininosuccinate synthase

Gene

arg12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei88 – 881CitrullineBy similarity
    Binding sitei93 – 931CitrullineBy similarity
    Binding sitei120 – 1201AspartateBy similarity
    Binding sitei124 – 1241AspartateBy similarity
    Binding sitei124 – 1241CitrullineBy similarity
    Binding sitei125 – 1251AspartateBy similarity
    Binding sitei128 – 1281CitrullineBy similarity
    Binding sitei181 – 1811CitrullineBy similarity
    Binding sitei190 – 1901CitrullineBy similarity
    Binding sitei269 – 2691CitrullineBy similarity
    Binding sitei281 – 2811CitrullineBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 199ATPBy similarity
    Nucleotide bindingi116 – 1249ATPBy similarity

    GO - Molecular functioni

    1. argininosuccinate synthase activity Source: PomBase
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. arginine biosynthetic process Source: PomBase
    2. argininosuccinate metabolic process Source: PomBase
    3. urea cycle Source: PomBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_214825. Urea cycle.
    UniPathwayiUPA00068; UER00113.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthase (EC:6.3.4.5)
    Alternative name(s):
    Citrulline--aspartate ligase
    Gene namesi
    Name:arg12
    ORF Names:SPBC428.05c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC428.05c.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Causes arginine auxotrophy.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Argininosuccinate synthasePRO_0000148558Add
    BLAST

    Proteomic databases

    MaxQBiO94354.
    PaxDbiO94354.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi277565. 43 interactions.
    MINTiMINT-4683443.
    STRINGi4896.SPBC428.05c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO94354.
    SMRiO94354. Positions 6-403.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0137.
    HOGENOMiHOG000230093.
    KOiK01940.
    OMAiPECEYIR.
    OrthoDBiEOG7KSXJR.
    PhylomeDBiO94354.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPiMF_00005. Arg_succ_synth_type1.
    InterProiIPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00764. Arginosuc_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00032. argG. 1 hit.
    PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94354-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQEVKRCVL AYSGGLDTSC ILAWLIEEGW EVICYMANVG QEEDWDAARE    50
    KALKVGAKKV YVEDLREEFI NDTVIPAAQA NAIYENVYLL GTSLARPIIA 100
    RRQIQIAEKE NCIAVSHGCT GKGNDQVRFE LAYYALKPDV QVIAPWRLPV 150
    FFERFAGRKD LLEYAAAKGI PVTQTTKKPW SMDENIVHCS YEAGILEDPS 200
    MTPPKDMWKL TVDPKDAPDE VEELSIHFEK GAPTKLECKD GTFSGVVSIF 250
    YQLNAIARRN GVGRIDIVEN RFSGLKSRGC YETPGLTILR TAHMDLEGLT 300
    MEREVRALRD QFVTFNLAKI LYNGQFFSPC TRMLLAANNV SQEVVNGVVK 350
    LSVYKGNVTV LGRKSDTAHL YDEKLSSMDE LGGFDPTWTS GFIQIESMRL 400
    RNSDEGKHWM 410
    Length:410
    Mass (Da):46,085
    Last modified:May 1, 1999 - v1
    Checksum:i1B3B873A4DCD0C21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA22280.1.
    PIRiT40457.
    RefSeqiNP_595183.1. NM_001021091.2.

    Genome annotation databases

    EnsemblFungiiSPBC428.05c.1; SPBC428.05c.1:pep; SPBC428.05c.
    GeneIDi2541050.
    KEGGispo:SPBC428.05c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA22280.1 .
    PIRi T40457.
    RefSeqi NP_595183.1. NM_001021091.2.

    3D structure databases

    ProteinModelPortali O94354.
    SMRi O94354. Positions 6-403.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277565. 43 interactions.
    MINTi MINT-4683443.
    STRINGi 4896.SPBC428.05c-1.

    Proteomic databases

    MaxQBi O94354.
    PaxDbi O94354.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC428.05c.1 ; SPBC428.05c.1:pep ; SPBC428.05c .
    GeneIDi 2541050.
    KEGGi spo:SPBC428.05c.

    Organism-specific databases

    PomBasei SPBC428.05c.

    Phylogenomic databases

    eggNOGi COG0137.
    HOGENOMi HOG000230093.
    KOi K01940.
    OMAi PECEYIR.
    OrthoDBi EOG7KSXJR.
    PhylomeDBi O94354.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00113 .
    Reactomei REACT_214825. Urea cycle.

    Miscellaneous databases

    NextBioi 20802164.
    PROi O94354.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPi MF_00005. Arg_succ_synth_type1.
    InterProi IPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00764. Arginosuc_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00032. argG. 1 hit.
    PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Development of a genetic transformation system using new selectable markers for fission yeast Schizosaccharomyces pombe."
      Fujita Y., Giga-Hama Y., Takegawa K.
      Yeast 22:193-202(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiASSY_SCHPO
    AccessioniPrimary (citable) accession number: O94354
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3