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O94354

- ASSY_SCHPO

UniProt

O94354 - ASSY_SCHPO

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Protein

Argininosuccinate synthase

Gene

arg12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei88 – 881CitrullineBy similarity
Binding sitei93 – 931CitrullineBy similarity
Binding sitei120 – 1201AspartateBy similarity
Binding sitei124 – 1241AspartateBy similarity
Binding sitei124 – 1241CitrullineBy similarity
Binding sitei125 – 1251AspartateBy similarity
Binding sitei128 – 1281CitrullineBy similarity
Binding sitei181 – 1811CitrullineBy similarity
Binding sitei190 – 1901CitrullineBy similarity
Binding sitei269 – 2691CitrullineBy similarity
Binding sitei281 – 2811CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 199ATPBy similarity
Nucleotide bindingi116 – 1249ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: PomBase
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine biosynthetic process Source: PomBase
  2. argininosuccinate metabolic process Source: PomBase
  3. urea cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_214825. Urea cycle.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:arg12
ORF Names:SPBC428.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC428.05c.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Causes arginine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Argininosuccinate synthasePRO_0000148558Add
BLAST

Proteomic databases

MaxQBiO94354.
PaxDbiO94354.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi277565. 44 interactions.
MINTiMINT-4683443.
STRINGi4896.SPBC428.05c-1.

Structurei

3D structure databases

ProteinModelPortaliO94354.
SMRiO94354. Positions 6-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiO94354.
KOiK01940.
OMAiPECEYIR.
OrthoDBiEOG7KSXJR.
PhylomeDBiO94354.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94354-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQEVKRCVL AYSGGLDTSC ILAWLIEEGW EVICYMANVG QEEDWDAARE
60 70 80 90 100
KALKVGAKKV YVEDLREEFI NDTVIPAAQA NAIYENVYLL GTSLARPIIA
110 120 130 140 150
RRQIQIAEKE NCIAVSHGCT GKGNDQVRFE LAYYALKPDV QVIAPWRLPV
160 170 180 190 200
FFERFAGRKD LLEYAAAKGI PVTQTTKKPW SMDENIVHCS YEAGILEDPS
210 220 230 240 250
MTPPKDMWKL TVDPKDAPDE VEELSIHFEK GAPTKLECKD GTFSGVVSIF
260 270 280 290 300
YQLNAIARRN GVGRIDIVEN RFSGLKSRGC YETPGLTILR TAHMDLEGLT
310 320 330 340 350
MEREVRALRD QFVTFNLAKI LYNGQFFSPC TRMLLAANNV SQEVVNGVVK
360 370 380 390 400
LSVYKGNVTV LGRKSDTAHL YDEKLSSMDE LGGFDPTWTS GFIQIESMRL
410
RNSDEGKHWM
Length:410
Mass (Da):46,085
Last modified:May 1, 1999 - v1
Checksum:i1B3B873A4DCD0C21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22280.1.
PIRiT40457.
RefSeqiNP_595183.1. NM_001021091.2.

Genome annotation databases

EnsemblFungiiSPBC428.05c.1; SPBC428.05c.1:pep; SPBC428.05c.
GeneIDi2541050.
KEGGispo:SPBC428.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22280.1 .
PIRi T40457.
RefSeqi NP_595183.1. NM_001021091.2.

3D structure databases

ProteinModelPortali O94354.
SMRi O94354. Positions 6-403.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277565. 44 interactions.
MINTi MINT-4683443.
STRINGi 4896.SPBC428.05c-1.

Proteomic databases

MaxQBi O94354.
PaxDbi O94354.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC428.05c.1 ; SPBC428.05c.1:pep ; SPBC428.05c .
GeneIDi 2541050.
KEGGi spo:SPBC428.05c.

Organism-specific databases

PomBasei SPBC428.05c.

Phylogenomic databases

eggNOGi COG0137.
HOGENOMi HOG000230093.
InParanoidi O94354.
KOi K01940.
OMAi PECEYIR.
OrthoDBi EOG7KSXJR.
PhylomeDBi O94354.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
Reactomei REACT_214825. Urea cycle.

Miscellaneous databases

NextBioi 20802164.
PROi O94354.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Development of a genetic transformation system using new selectable markers for fission yeast Schizosaccharomyces pombe."
    Fujita Y., Giga-Hama Y., Takegawa K.
    Yeast 22:193-202(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiASSY_SCHPO
AccessioniPrimary (citable) accession number: O94354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3