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Protein

Argininosuccinate synthase

Gene

arg12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase, mitochondrial (arg3)
  2. Argininosuccinate synthase (arg12)
  3. Probable argininosuccinate lyase (argx), Argininosuccinate lyase (arg7)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei88CitrullineBy similarity1
Binding sitei93CitrullineBy similarity1
Binding sitei120AspartateBy similarity1
Binding sitei124AspartateBy similarity1
Binding sitei124CitrullineBy similarity1
Binding sitei125AspartateBy similarity1
Binding sitei128CitrullineBy similarity1
Binding sitei181CitrullineBy similarity1
Binding sitei190CitrullineBy similarity1
Binding sitei269CitrullineBy similarity1
Binding sitei281CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 19ATPBy similarity9
Nucleotide bindingi116 – 124ATPBy similarity9

GO - Molecular functioni

  • argininosuccinate synthase activity Source: PomBase
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • arginine biosynthetic process Source: PomBase
  • argininosuccinate metabolic process Source: PomBase
  • urea cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:arg12
ORF Names:SPBC428.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC428.05c.
PomBaseiSPBC428.05c. arg12.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Causes arginine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485581 – 410Argininosuccinate synthaseAdd BLAST410

Proteomic databases

MaxQBiO94354.
PRIDEiO94354.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi277565. 46 interactors.
MINTiMINT-4683443.

Structurei

3D structure databases

ProteinModelPortaliO94354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000230093.
InParanoidiO94354.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiEOG092C2BM5.
PhylomeDBiO94354.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQEVKRCVL AYSGGLDTSC ILAWLIEEGW EVICYMANVG QEEDWDAARE
60 70 80 90 100
KALKVGAKKV YVEDLREEFI NDTVIPAAQA NAIYENVYLL GTSLARPIIA
110 120 130 140 150
RRQIQIAEKE NCIAVSHGCT GKGNDQVRFE LAYYALKPDV QVIAPWRLPV
160 170 180 190 200
FFERFAGRKD LLEYAAAKGI PVTQTTKKPW SMDENIVHCS YEAGILEDPS
210 220 230 240 250
MTPPKDMWKL TVDPKDAPDE VEELSIHFEK GAPTKLECKD GTFSGVVSIF
260 270 280 290 300
YQLNAIARRN GVGRIDIVEN RFSGLKSRGC YETPGLTILR TAHMDLEGLT
310 320 330 340 350
MEREVRALRD QFVTFNLAKI LYNGQFFSPC TRMLLAANNV SQEVVNGVVK
360 370 380 390 400
LSVYKGNVTV LGRKSDTAHL YDEKLSSMDE LGGFDPTWTS GFIQIESMRL
410
RNSDEGKHWM
Length:410
Mass (Da):46,085
Last modified:May 1, 1999 - v1
Checksum:i1B3B873A4DCD0C21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22280.1.
PIRiT40457.
RefSeqiNP_595183.1. NM_001021091.2.

Genome annotation databases

EnsemblFungiiSPBC428.05c.1; SPBC428.05c.1:pep; SPBC428.05c.
GeneIDi2541050.
KEGGispo:SPBC428.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA22280.1.
PIRiT40457.
RefSeqiNP_595183.1. NM_001021091.2.

3D structure databases

ProteinModelPortaliO94354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277565. 46 interactors.
MINTiMINT-4683443.

Proteomic databases

MaxQBiO94354.
PRIDEiO94354.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC428.05c.1; SPBC428.05c.1:pep; SPBC428.05c.
GeneIDi2541050.
KEGGispo:SPBC428.05c.

Organism-specific databases

EuPathDBiFungiDB:SPBC428.05c.
PomBaseiSPBC428.05c. arg12.

Phylogenomic databases

HOGENOMiHOG000230093.
InParanoidiO94354.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiEOG092C2BM5.
PhylomeDBiO94354.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Miscellaneous databases

PROiO94354.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_SCHPO
AccessioniPrimary (citable) accession number: O94354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.