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Protein

ATP-dependent (S)-NAD(P)H-hydrate dehydratase

Gene

SPCC61.03

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.UniRule annotation

Catalytic activityi

ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH.UniRule annotation
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH.

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121NAD(P)HX; via amide nitrogenUniRule annotation1
Binding sitei238NAD(P)HXUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 213ATPUniRule annotation5
Nucleotide bindingi228 – 237ATPUniRule annotation10

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-197264. Nicotinamide salvaging.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation)
Alternative name(s):
ATP-dependent NAD(P)HX dehydrataseUniRule annotation
Gene namesi
ORF Names:SPCC61.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC61.03.
PomBaseiSPCC61.03.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003159591 – 327ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST327

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94347.
PRIDEiO94347.

Interactioni

Protein-protein interaction databases

BioGridi276063. 14 interactors.
MINTiMINT-4683424.

Structurei

3D structure databases

ProteinModelPortaliO94347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 313YjeF C-terminalUniRule annotationAdd BLAST303

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 180NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrD/CARKD family.UniRule annotation
Contains 1 YjeF C-terminal domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000163126.
InParanoidiO94347.
KOiK17757.
OMAiSMTASDM.
OrthoDBiEOG092C4N4W.
PhylomeDBiO94347.

Family and domain databases

CDDicd01171. YXKO-related. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
InterProiIPR017953. Carbohydrate_kinase_pred_CS.
IPR000631. CARKD.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00196. yjeF_cterm. 1 hit.
PROSITEiPS01049. YJEF_C_1. 1 hit.
PS51383. YJEF_C_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSGSPKITN LLTRVKRIIP PLLDTFHKGQ AGRVGVFGGC QHYTGAPYYS
60 70 80 90 100
SMSSMLFGSD QSHIFCEKEA ANVIKSYSPD LIVHPFLREK DKAGPEDSVD
110 120 130 140 150
KCFELIKPMM GRLHAIVIGP GLGRDEWMQE IMAKVIEYAR KNDMPMVIDA
160 170 180 190 200
DGLWLIQQRP ELVSGYHNVI LTPNVIEFKR LCDKLDIKSD GPDACNQLAG
210 220 230 240 250
KLNLLIIQKG QSDIISDGAT AYACSVPGGL KRCGGQGDIL TGILATFLAW
260 270 280 290 300
RHAYLSKEWD TEGNMDAKEC LFLAAFGASA CTRWCSRLAF KECGRATQST
310 320
DLVRHVGKAY NALMEDEIPS VEEKIKD
Length:327
Mass (Da):36,094
Last modified:May 1, 1999 - v1
Checksum:i5DB8746FD47D75A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA22272.1.
PIRiT41463.
RefSeqiNP_588194.1. NM_001023184.2.

Genome annotation databases

EnsemblFungiiSPCC61.03.1; SPCC61.03.1:pep; SPCC61.03.
GeneIDi2539500.
KEGGispo:SPCC61.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA22272.1.
PIRiT41463.
RefSeqiNP_588194.1. NM_001023184.2.

3D structure databases

ProteinModelPortaliO94347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276063. 14 interactors.
MINTiMINT-4683424.

Proteomic databases

MaxQBiO94347.
PRIDEiO94347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC61.03.1; SPCC61.03.1:pep; SPCC61.03.
GeneIDi2539500.
KEGGispo:SPCC61.03.

Organism-specific databases

EuPathDBiFungiDB:SPCC61.03.
PomBaseiSPCC61.03.

Phylogenomic databases

HOGENOMiHOG000163126.
InParanoidiO94347.
KOiK17757.
OMAiSMTASDM.
OrthoDBiEOG092C4N4W.
PhylomeDBiO94347.

Enzyme and pathway databases

ReactomeiR-SPO-197264. Nicotinamide salvaging.

Miscellaneous databases

PROiO94347.

Family and domain databases

CDDicd01171. YXKO-related. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
InterProiIPR017953. Carbohydrate_kinase_pred_CS.
IPR000631. CARKD.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00196. yjeF_cterm. 1 hit.
PROSITEiPS01049. YJEF_C_1. 1 hit.
PS51383. YJEF_C_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNRD_SCHPO
AccessioniPrimary (citable) accession number: O94347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: October 5, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.