Reviewed, UniProtKB/Swiss-Prot O94335 (STT3_SCHPO)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3
      Short name=Oligosaccharyl transferase subunit stt3
    EC=2.4.1.119

Gene names

Name:	stt3
ORF Names:	SPBC1271.02

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER) By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the STT3 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 752	752	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3		PRO_0000072292
Regions
	Transmembrane	20 – 42	23	Potential
	Transmembrane	92 – 114	23	Potential
	Transmembrane	121 – 140	20	Potential
	Transmembrane	150 – 169	20	Potential
	Transmembrane	176 – 193	18	Potential
	Transmembrane	213 – 235	23	Potential
	Transmembrane	242 – 264	23	Potential
	Transmembrane	274 – 296	23	Potential
	Transmembrane	308 – 330	23	Potential
	Transmembrane	363 – 385	23	Potential
	Transmembrane	392 – 414	23	Potential
Experimental info
	Sequence conflict	399	1	S → P in BAA76479. Ref.1
	Sequence conflict	461	1	F → S in BAA76479. Ref.1
	Sequence conflict	739 – 752	14	LRRPA…IPLRV → SAVLQKLTKFL Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O94335-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2ACB97DFE82B10B8	FASTA	752	84,974
	10 20 30 40 50 60 MANSATITSK KGVKSHQKDW KIPLKVLILI CIAVASVSSR LFSVIRYESI IHEFDPWFNF 70 80 90 100 110 120 RASKILVEQG FYNFLNWFDE RSWYPLGRVA GGTLYPGLMV TSGIIFKVLH LLRINVNIRD 130 140 150 160 170 180 VCVLLAPAFS GITAIATYYL ARELKSDACG LLAAAFMGIA PGYTSRSVAG SYDNEAIAIT 190 200 210 220 230 240 LLMSTFALWI KAVKSGSSFW GACTGLLYFY MVTAWGGYVF ITNMIPLHVF VLLLMGRYTS 250 260 270 280 290 300 KLYIAYTTYY VIGTLASMQV PFVGFQPVST SEHMSALGVF GLLQLFAFYN YVKGLVSSKQ 310 320 330 340 350 360 FQILIRFALV CLVGLATVVL FALSSTGVIA PWTGRFYSLW DTNYAKIHIP IIASVSEHQP 370 380 390 400 410 420 PTWSSLFFDL QFLIWLLPVG VYLCFKELRN EHVFIIIYSV LGTYFCGVMV RLVLTLTPCV 430 440 450 460 470 480 CIAAAVAIST LLDTYMGPEV EEDKVSEEAA SAKSKNKKGI FSILSFFTSG SKNIGIYSLL 490 500 510 520 530 540 SRVLVISSTA YFLIMFVYHS SWVTSNAYSS PTVVLSTVLN DGSLMYIDDF REAYDWLRRN 550 560 570 580 590 600 TPYDTKVMSW WDYGYQIAGM ADRITLVDNN TWNNTHIATV GKAMSSPEEK AYPILRKHDV 610 620 630 640 650 660 DYILIIYGGT LGYSSDDMNK FLWMIRISQG LWPDEIVERN FFTPNGEYRT DDAATPTMRE 670 680 690 700 710 720 SLLYKMSYHG AWKLFPPNQG YDRARNQKLP SKDPQLFTIE EAFTTVHHLV RLYKVKKPDT 730 740 750 LGRDLKQVTL FEEGKRKKLR RPAKTNEIPL RV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Schizosaccharomyces pombe stt3+ is a functional homologue of Saccharomyces cerevisiae STT3 which regulates oligosaccharyltransferase activity." Yoshida S., Matsuura A., Merregaert J., Anraku Y. Yeast 15:497-505(1999) [PubMed: 10234787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015232 Genomic DNA. Translation: BAA76479.1. CU329671 Genomic DNA. Translation: CAA22192.1.
PIR	T39338. T43370.
RefSeq	NP_595148.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O94335.
Protein family/group databases
CAZy	GT66. Glycosyltransferase Family 66.
Genome annotation databases
GeneID	2540190.
GenomeReviews	Gene locus stt3 in contig CU329671_GR.
KEGG	spo:SPBC1271.02.
NMPDR	fig|4896.1.peg.1014.
Organism-specific databases
GeneDB_Spombe	SPBC1271.02.
Phylogenomic databases
eggNOG	fuNOG05891.
HOGENOM	HBG314442.
OMA	IDIRNIC.
OrthoDB	EOG9M670P.
PhylomeDB	O94335.
Enzyme and pathway databases
BRENDA	2.4.1.119. 653.
Gene expression databases
ArrayExpress	O94335.
Family and domain databases
InterPro	IPR003674. Oligo_trans_STT3. [Graphical view]
Pfam	PF02516. STT3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STT3_SCHPO

Accession

Primary (citable) accession number: O94335
Secondary accession number(s): Q8WZK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	May 1, 1999
Last modified:	January 19, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)