O94335 (STT3_SCHPO) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 69.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 Short name=Oligosaccharyl transferase subunit stt3 EC=2.4.1.119 | ||||
| Gene names |
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| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ||||
| Taxonomic identifier | 284812 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 752 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER) By similarity. |
| Catalytic activity | Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine. |
| Pathway | |
| Subunit structure | Component of the oligosaccharyltransferase (OST) complex By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the STT3 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | protein N-linked glycosylation Inferred from genetic interaction. Source: GeneDB_Spombe |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complexInferred from genetic interaction. Source: GeneDB_Spombe |
| Molecular function | dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 752 | 752 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 | PRO_0000072292 | |||||
Regions | |||||||||
| Transmembrane | 20 – 42 | 23 | Helical; Potential | ||||||
| Transmembrane | 92 – 114 | 23 | Helical; Potential | ||||||
| Transmembrane | 121 – 140 | 20 | Helical; Potential | ||||||
| Transmembrane | 150 – 169 | 20 | Helical; Potential | ||||||
| Transmembrane | 176 – 193 | 18 | Helical; Potential | ||||||
| Transmembrane | 213 – 235 | 23 | Helical; Potential | ||||||
| Transmembrane | 242 – 264 | 23 | Helical; Potential | ||||||
| Transmembrane | 274 – 296 | 23 | Helical; Potential | ||||||
| Transmembrane | 308 – 330 | 23 | Helical; Potential | ||||||
| Transmembrane | 363 – 385 | 23 | Helical; Potential | ||||||
| Transmembrane | 392 – 414 | 23 | Helical; Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 399 | 1 | S → P in BAA76479. Ref.1 | ||||||
| Sequence conflict | 461 | 1 | F → S in BAA76479. Ref.1 | ||||||
| Sequence conflict | 739 – 752 | 14 | LRRPA…IPLRV → SAVLQKLTKFL in BAA76479. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Schizosaccharomyces pombe stt3+ is a functional homologue of Saccharomyces cerevisiae STT3 which regulates oligosaccharyltransferase activity." Yoshida S., Matsuura A., Merregaert J., Anraku Y. Yeast 15:497-505(1999) [PubMed: 10234787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB015232 Genomic DNA. Translation: BAA76479.1. CU329671 Genomic DNA. Translation: CAA22192.1. |
| PIR | T39338. T43370. |
| RefSeq | NP_595148.1. NM_001021056.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O94335. |
Protein family/group databases | |
| CAZy | GT66. Glycosyltransferase Family 66. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | SPBC1271.02.1; SPBC1271.02.1:pep; SPBC1271.02. |
| GeneID | 2540190. |
| GenomeReviews | Gene locus stt3 in contig CU329671_GR. |
| KEGG | spo:SPBC1271.02. |
| NMPDR | fig|4896.1.peg.1014. |
Organism-specific databases | |
| GeneDB_Spombe | SPBC1271.02. |
Phylogenomic databases | |
| eggNOG | fuNOG05891. |
| GeneTree | EFGT00050000005386. |
| HOGENOM | HBG314442. |
| OMA | YIDDFRE. |
| OrthoDB | EOG4N6052. |
Gene expression databases | |
| ArrayExpress | O94335. |
Family and domain databases | |
| InterPro | IPR003674. Oligo_trans_STT3. [Graphical view] |
| KO | K07151. |
| Pfam | PF02516. STT3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | STT3_SCHPO | ||||||||
| Accession | Primary (citable) accession number: O94335 Secondary accession number(s): Q8WZK7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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