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O94316 (SN114_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor cwf10
Alternative name(s):
114 kDa U5 small nuclear ribonucleoprotein component homolog
Complexed with cdc5 protein 10
Gene names
Name:cwf10
Synonyms:snu114, spef2
ORF Names:SPBC215.12
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length984 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

Subunit structure

Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2. Ref.3 Ref.4

Subcellular location

Cytoplasm. Nucleus Ref.5.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

cdc5P399645EBI-538866,EBI-538771
SPBC20F10.05O429753EBI-538866,EBI-9003631

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 984984Pre-mRNA-splicing factor cwf10
PRO_0000363370

Regions

Nucleotide binding148 – 1558GTP By similarity
Nucleotide binding216 – 2205GTP By similarity
Nucleotide binding270 – 2734GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
O94316 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: B15A139BEC1EA53E

FASTA984111,306
        10         20         30         40         50         60 
MMEEDLYDEF GNYIGPENEE DEEELFPQAP SPTIAQVPSF EEVIPDEELE DVERAEEMAL 

        70         80         90        100        110        120 
SHLEPQNAVV LHEDKQYYPS AEEVYGSNVD IMVQEQDTQP LSQPIIEPIR HKRIAIETTN 

       130        140        150        160        170        180 
VPDTVYKKEF LFGLLTGTDD VRSFIVAGHL HHGKSALLDL LVYYTHPDTK PPKRRSLRYT 

       190        200        210        220        230        240 
DTHYLERERV MSIKSTPLTL AVSDMKGKTF AFQCIDTPGH VDFVDEVAAP MAISDGVVLV 

       250        260        270        280        290        300 
VDVIEGVMIN TTRIIKHAIL HDMPIVLVLN KVDRLILELR LPPNDAYHKL RHVIDEVNDN 

       310        320        330        340        350        360 
ICQISKDLKY RVSPELGNVC FASCDLGYCF TLSSFAKLYI DRHGGIDVDL FSKRLWGDIY 

       370        380        390        400        410        420 
FDSKTRKFAK QSLDGSGVRS FVHFILEPLY KLHTLTISDE AEKLKKHLSS FQIYLKPKDY 

       430        440        450        460        470        480 
LLDPKPLLQL ICASFFGFPV GFVNAVTRHI PSPRENAARK ASQSYIGPIN SSIGKAILEM 

       490        500        510        520        530        540 
SREESAPLVM HVTKLYNTVD ANNFYAFARV YSGQVKKGQK VKVLGENYSL EDEEDMVVAH 

       550        560        570        580        590        600 
IAEICVPCAR YRLHVDGAVA GMLVLLGGVD NSISKTATIV SDNLKDDPYI FRPIAHMSES 

       610        620        630        640        650        660 
VFKVAVEPHN PSELPKLLDG LRKTNKSYPL SITKVEESGE HTIFGTGEMY MDCLLYDLRT 

       670        680        690        700        710        720 
LYSEIEIRVS DPVARFCETA VDTSSIKCFS DTPNKKNRIT MVVEPLEKGI SNDIENGKVN 

       730        740        750        760        770        780 
INWPQKRISE FFQKNYDWDL LASRSIWAFG PDDRGTNILR DDTLSTDVDK NVLNSVKEYI 

       790        800        810        820        830        840 
KQGFQWGTRE GPLCDETIRN VNFRLMDVVL APEQIYRGGG QIIPTARRVC YSSFLTASPR 

       850        860        870        880        890        900 
LMEPVYMVEV HAPADSLPII YDLLTRRRGH VLQDIPRPGS PLYLVRALIP VIDSCGFETD 

       910        920        930        940        950        960 
LRVHTQGQAM CQMVFDHWQV VPGDPLDKSI KPKPLEPARG SDLARDFLIK TRRRKGLVED 

       970        980 
VSTTRYFDQE MIDSLKEAGV VLSL 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Comparative functional genomics of the fission yeasts."
Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K. expand/collapse author list , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: REVISION OF GENE MODEL.
[3]"Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing."
McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.
Mol. Cell. Biol. 19:5352-5362(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAA22126.2.
PIRT39902.
RefSeqNP_596689.2. NM_001022612.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277234. 50 interactions.
IntActO94316. 11 interactions.
MINTMINT-4683154.
STRING4896.SPBC215.12-1.

Proteomic databases

PaxDbO94316.
PRIDEO94316.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2540711.
KEGGspo:SPBC215.12.

Organism-specific databases

PomBaseSPBC215.12.

Phylogenomic databases

eggNOGCOG0480.
HOGENOMHOG000231589.
KOK12852.
OrthoDBEOG7966SQ.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Other

NextBio20801833.
PROO94316.

Entry information

Entry nameSN114_SCHPO
AccessionPrimary (citable) accession number: O94316
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names