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O94316

- SN114_SCHPO

UniProt

O94316 - SN114_SCHPO

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Protein

Pre-mRNA-splicing factor cwf10

Gene
cwf10, snu114, spef2, SPBC215.12
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi148 – 1558GTP By similarity
Nucleotide bindingi216 – 2205GTP By similarity
Nucleotide bindingi270 – 2734GTP By similarity

GO - Molecular functioni

  1. GTPase activity Source: PomBase
  2. GTP binding Source: PomBase
  3. protein binding Source: IntAct

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. mRNA cis splicing, via spliceosome Source: PomBase
  3. regulation of heterochromatin domain assembly Source: PomBase
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_224835. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor cwf10
Alternative name(s):
114 kDa U5 small nuclear ribonucleoprotein component homolog
Complexed with cdc5 protein 10
Gene namesi
Name:cwf10
Synonyms:snu114, spef2
ORF Names:SPBC215.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC215.12.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. nucleus Source: PomBase
  3. spliceosomal complex Source: PomBase
  4. U4/U6 x U5 tri-snRNP complex Source: PomBase
  5. U5 snRNP Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 984984Pre-mRNA-splicing factor cwf10PRO_0000363370Add
BLAST

Proteomic databases

MaxQBiO94316.
PaxDbiO94316.
PRIDEiO94316.

Interactioni

Subunit structurei

Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc5P399645EBI-538866,EBI-538771
SPBC20F10.05O429753EBI-538866,EBI-9003631

Protein-protein interaction databases

BioGridi277234. 85 interactions.
IntActiO94316. 11 interactions.
MINTiMINT-4683154.
STRINGi4896.SPBC215.12-1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini139 – 402264tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1558G1 By similarity
Regioni190 – 1945G2 By similarity
Regioni216 – 2194G3 By similarity
Regioni270 – 2734G4 By similarity
Regioni371 – 3733G5 By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0480.
HOGENOMiHOG000231589.
KOiK12852.
OrthoDBiEOG7966SQ.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94316-1 [UniParc]FASTAAdd to Basket

« Hide

MMEEDLYDEF GNYIGPENEE DEEELFPQAP SPTIAQVPSF EEVIPDEELE    50
DVERAEEMAL SHLEPQNAVV LHEDKQYYPS AEEVYGSNVD IMVQEQDTQP 100
LSQPIIEPIR HKRIAIETTN VPDTVYKKEF LFGLLTGTDD VRSFIVAGHL 150
HHGKSALLDL LVYYTHPDTK PPKRRSLRYT DTHYLERERV MSIKSTPLTL 200
AVSDMKGKTF AFQCIDTPGH VDFVDEVAAP MAISDGVVLV VDVIEGVMIN 250
TTRIIKHAIL HDMPIVLVLN KVDRLILELR LPPNDAYHKL RHVIDEVNDN 300
ICQISKDLKY RVSPELGNVC FASCDLGYCF TLSSFAKLYI DRHGGIDVDL 350
FSKRLWGDIY FDSKTRKFAK QSLDGSGVRS FVHFILEPLY KLHTLTISDE 400
AEKLKKHLSS FQIYLKPKDY LLDPKPLLQL ICASFFGFPV GFVNAVTRHI 450
PSPRENAARK ASQSYIGPIN SSIGKAILEM SREESAPLVM HVTKLYNTVD 500
ANNFYAFARV YSGQVKKGQK VKVLGENYSL EDEEDMVVAH IAEICVPCAR 550
YRLHVDGAVA GMLVLLGGVD NSISKTATIV SDNLKDDPYI FRPIAHMSES 600
VFKVAVEPHN PSELPKLLDG LRKTNKSYPL SITKVEESGE HTIFGTGEMY 650
MDCLLYDLRT LYSEIEIRVS DPVARFCETA VDTSSIKCFS DTPNKKNRIT 700
MVVEPLEKGI SNDIENGKVN INWPQKRISE FFQKNYDWDL LASRSIWAFG 750
PDDRGTNILR DDTLSTDVDK NVLNSVKEYI KQGFQWGTRE GPLCDETIRN 800
VNFRLMDVVL APEQIYRGGG QIIPTARRVC YSSFLTASPR LMEPVYMVEV 850
HAPADSLPII YDLLTRRRGH VLQDIPRPGS PLYLVRALIP VIDSCGFETD 900
LRVHTQGQAM CQMVFDHWQV VPGDPLDKSI KPKPLEPARG SDLARDFLIK 950
TRRRKGLVED VSTTRYFDQE MIDSLKEAGV VLSL 984
Length:984
Mass (Da):111,306
Last modified:October 3, 2012 - v2
Checksum:iB15A139BEC1EA53E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329671 Genomic DNA. Translation: CAA22126.2.
PIRiT39902.
RefSeqiNP_596689.2. NM_001022612.2.

Genome annotation databases

EnsemblFungiiSPBC215.12.1; SPBC215.12.1:pep; SPBC215.12.
GeneIDi2540711.
KEGGispo:SPBC215.12.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329671 Genomic DNA. Translation: CAA22126.2 .
PIRi T39902.
RefSeqi NP_596689.2. NM_001022612.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277234. 85 interactions.
IntActi O94316. 11 interactions.
MINTi MINT-4683154.
STRINGi 4896.SPBC215.12-1.

Proteomic databases

MaxQBi O94316.
PaxDbi O94316.
PRIDEi O94316.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC215.12.1 ; SPBC215.12.1:pep ; SPBC215.12 .
GeneIDi 2540711.
KEGGi spo:SPBC215.12.

Organism-specific databases

PomBasei SPBC215.12.

Phylogenomic databases

eggNOGi COG0480.
HOGENOMi HOG000231589.
KOi K12852.
OrthoDBi EOG7966SQ.

Enzyme and pathway databases

Reactomei REACT_224835. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi 20801833.
PROi O94316.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SMARTi SM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Comparative functional genomics of the fission yeasts."
    Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
    , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
    Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  3. "Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing."
    McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.
    Mol. Cell. Biol. 19:5352-5362(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSN114_SCHPO
AccessioniPrimary (citable) accession number: O94316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 3, 2012
Last modified: September 3, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi