Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O94316

- SN114_SCHPO

UniProt

O94316 - SN114_SCHPO

Protein

Pre-mRNA-splicing factor cwf10

Gene

cwf10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi148 – 1558GTPBy similarity
    Nucleotide bindingi216 – 2205GTPBy similarity
    Nucleotide bindingi270 – 2734GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: PomBase
    2. GTP binding Source: PomBase
    3. protein binding Source: IntAct

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. mRNA cis splicing, via spliceosome Source: PomBase
    3. regulation of heterochromatin domain assembly Source: PomBase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_224835. mRNA Splicing - Minor Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor cwf10
    Alternative name(s):
    114 kDa U5 small nuclear ribonucleoprotein component homolog
    Complexed with cdc5 protein 10
    Gene namesi
    Name:cwf10
    Synonyms:snu114, spef2
    ORF Names:SPBC215.12
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC215.12.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. nucleus Source: PomBase
    3. spliceosomal complex Source: PomBase
    4. U4/U6 x U5 tri-snRNP complex Source: PomBase
    5. U5 snRNP Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 984984Pre-mRNA-splicing factor cwf10PRO_0000363370Add
    BLAST

    Proteomic databases

    MaxQBiO94316.
    PaxDbiO94316.
    PRIDEiO94316.

    Interactioni

    Subunit structurei

    Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    cdc5P399645EBI-538866,EBI-538771
    SPBC20F10.05O429753EBI-538866,EBI-9003631

    Protein-protein interaction databases

    BioGridi277234. 85 interactions.
    IntActiO94316. 11 interactions.
    MINTiMINT-4683154.
    STRINGi4896.SPBC215.12-1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini139 – 402264tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1558G1PROSITE-ProRule annotation
    Regioni190 – 1945G2PROSITE-ProRule annotation
    Regioni216 – 2194G3PROSITE-ProRule annotation
    Regioni270 – 2734G4PROSITE-ProRule annotation
    Regioni371 – 3733G5PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0480.
    HOGENOMiHOG000231589.
    KOiK12852.
    OrthoDBiEOG7966SQ.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94316-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMEEDLYDEF GNYIGPENEE DEEELFPQAP SPTIAQVPSF EEVIPDEELE    50
    DVERAEEMAL SHLEPQNAVV LHEDKQYYPS AEEVYGSNVD IMVQEQDTQP 100
    LSQPIIEPIR HKRIAIETTN VPDTVYKKEF LFGLLTGTDD VRSFIVAGHL 150
    HHGKSALLDL LVYYTHPDTK PPKRRSLRYT DTHYLERERV MSIKSTPLTL 200
    AVSDMKGKTF AFQCIDTPGH VDFVDEVAAP MAISDGVVLV VDVIEGVMIN 250
    TTRIIKHAIL HDMPIVLVLN KVDRLILELR LPPNDAYHKL RHVIDEVNDN 300
    ICQISKDLKY RVSPELGNVC FASCDLGYCF TLSSFAKLYI DRHGGIDVDL 350
    FSKRLWGDIY FDSKTRKFAK QSLDGSGVRS FVHFILEPLY KLHTLTISDE 400
    AEKLKKHLSS FQIYLKPKDY LLDPKPLLQL ICASFFGFPV GFVNAVTRHI 450
    PSPRENAARK ASQSYIGPIN SSIGKAILEM SREESAPLVM HVTKLYNTVD 500
    ANNFYAFARV YSGQVKKGQK VKVLGENYSL EDEEDMVVAH IAEICVPCAR 550
    YRLHVDGAVA GMLVLLGGVD NSISKTATIV SDNLKDDPYI FRPIAHMSES 600
    VFKVAVEPHN PSELPKLLDG LRKTNKSYPL SITKVEESGE HTIFGTGEMY 650
    MDCLLYDLRT LYSEIEIRVS DPVARFCETA VDTSSIKCFS DTPNKKNRIT 700
    MVVEPLEKGI SNDIENGKVN INWPQKRISE FFQKNYDWDL LASRSIWAFG 750
    PDDRGTNILR DDTLSTDVDK NVLNSVKEYI KQGFQWGTRE GPLCDETIRN 800
    VNFRLMDVVL APEQIYRGGG QIIPTARRVC YSSFLTASPR LMEPVYMVEV 850
    HAPADSLPII YDLLTRRRGH VLQDIPRPGS PLYLVRALIP VIDSCGFETD 900
    LRVHTQGQAM CQMVFDHWQV VPGDPLDKSI KPKPLEPARG SDLARDFLIK 950
    TRRRKGLVED VSTTRYFDQE MIDSLKEAGV VLSL 984
    Length:984
    Mass (Da):111,306
    Last modified:October 3, 2012 - v2
    Checksum:iB15A139BEC1EA53E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA22126.2.
    PIRiT39902.
    RefSeqiNP_596689.2. NM_001022612.2.

    Genome annotation databases

    EnsemblFungiiSPBC215.12.1; SPBC215.12.1:pep; SPBC215.12.
    GeneIDi2540711.
    KEGGispo:SPBC215.12.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA22126.2 .
    PIRi T39902.
    RefSeqi NP_596689.2. NM_001022612.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277234. 85 interactions.
    IntActi O94316. 11 interactions.
    MINTi MINT-4683154.
    STRINGi 4896.SPBC215.12-1.

    Proteomic databases

    MaxQBi O94316.
    PaxDbi O94316.
    PRIDEi O94316.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC215.12.1 ; SPBC215.12.1:pep ; SPBC215.12 .
    GeneIDi 2540711.
    KEGGi spo:SPBC215.12.

    Organism-specific databases

    PomBasei SPBC215.12.

    Phylogenomic databases

    eggNOGi COG0480.
    HOGENOMi HOG000231589.
    KOi K12852.
    OrthoDBi EOG7966SQ.

    Enzyme and pathway databases

    Reactomei REACT_224835. mRNA Splicing - Minor Pathway.

    Miscellaneous databases

    NextBioi 20801833.
    PROi O94316.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Comparative functional genomics of the fission yeasts."
      Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
      , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
      Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.
    3. "Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing."
      McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.
      Mol. Cell. Biol. 19:5352-5362(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSN114_SCHPO
    AccessioniPrimary (citable) accession number: O94316
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3