Reviewed, UniProtKB/Swiss-Prot O94307 (PFD5_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable prefoldin subunit 5
Alternative name(s):
    Byr1-binding protein Bob1

Gene names

Name:	bob1
ORF Names:	SPBC215.02

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins By similarity. Required for normal cytoskeletal function and when bound to byr1, is involved in the regulation of sexual differentiation.
Subunit structure	Heterohexamer of two PFD-alpha type and four PFD-beta type subunits By similarity. Interacts with byr1.
Subcellular location	Cytoplasm. Note: Localizes to the cell tips and septum forming regions. Ref.1
Sequence similarities	Belongs to the prefoldin subunit alpha family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from direct assay. Source: GeneDB_SPombe prefoldin complex Inferred from electronic annotation. Source: InterPro
Molecular function	unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
byr1	P10506	1	EBI-2042611,EBI-2042633

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Probable prefoldin subunit 5		PRO_0000153667

Sequences

	Sequence		Length	Mass (Da)
	O94307-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 7EDDC52E8D4FFD80	FASTA	154	16,774
	10 20 30 40 50 60 MAEGNKAVDL TSLSLEQLSE VIKQLDSELE YLSTSYGQLG RAQLKFRECL ANVNDAVRAE 70 80 90 100 110 120 NDGKEVLVPL TSSLYVPGKL NLGNSKLLVD IGTGYYVEKS AGEATEYYKR KCEYLASSIE 130 140 150 NLNNAIDAKS VQIRAVQNIM QQKATATTAA TKSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe." Henkel J., Du H., Yang P., Qyang Y., Kansra S., Ko M., Kim H., Marcus S. Differentiation 67:98-106(2001) [PubMed: 11683500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH BYR1, SUBCELLULAR LOCATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA22116.1. AY035821 Genomic DNA. Translation: AAK60340.1.
PIR	T39892.
RefSeq	NP_596679.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O94307. 1 interaction.
STRING	O94307.
Genome annotation databases
GeneID	2540664.
GenomeReviews	Gene locus bob1 in contig CU329671_GR.
KEGG	spo:SPBC215.02.
NMPDR	fig|4896.1.peg.2545.
Organism-specific databases
GeneDB_Spombe	SPBC215.02.
Phylogenomic databases
OMA	KRRTRAY
OrthoDB	EOG9WWT1X
Gene expression databases
ArrayExpress	O94307.
Family and domain databases
InterPro	IPR011599. PFD_alpha. IPR004127. PFD_alpha-like. IPR009053. Prefoldin. [Graphical view]
Pfam	PF02996. Prefoldin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00293. PFD_alpha. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PFD5_SCHPO

Accession

Primary (citable) accession number: O94307

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)