Reviewed, UniProtKB/Swiss-Prot O94277 (PABS_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative aminodeoxychorismate synthase
      Short name=ADC synthase
    EC=2.6.1.85
Alternative name(s):
    Para-aminobenzoate synthase
    P-aminobenzoic acid synthase
      Short name=PABA synthase

Gene names

ORF Names:

SPBP8B7.29

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine. Required for the synthesis of 4-aminobenzoate (PABA), an important component in tetrahydrofolate biosynthesis By similarity.
Catalytic activity	Chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.
Subcellular location	Cytoplasm. Nucleus. Ref.2
Sequence similarities	In the C-terminal section; belongs to the anthranilate synthase component I family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Folate biosynthesis
Cellular component	Cytoplasm Nucleus
Domain	Glutamine amidotransferase
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.2 Inferred from direct assay. Source: GeneDB_SPombe nucleus Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	4-amino-4-deoxychorismate synthase activity Inferred from electronic annotation. Source: EC anthranilate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Putative aminodeoxychorismate synthase		PRO_0000310363
Regions
	Domain	9 – 203	195	Glutamine amidotransferase type-1
	Region	266 – 718	453	PABB component By similarity
Sites
	Active site	88	1	Nucleophile By similarity
	Active site	177	1	By similarity
	Active site	179	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94277-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 933AE5EF1F1D3820	FASTA	718	80,598
	10 20 30 40 50 60 MSEISNRLQI LLIDCYDSYT FNLYDLLYKA SENACVIVVH WDKMSPDLWE DILQFDAIVV 70 80 90 100 110 120 GPGPGHPAEY SSILNRIWQL NIPVMGICLG FQSLALYHGA TIERMPNLPW HGRVSSVTTS 130 140 150 160 170 180 KTFIFDGISA VKGMRYHSLY ANKIPIDSLQ ILAQSDEDNI VMSIKATKFP HFGILYHPES 190 200 210 220 230 240 VGSSKSLKIF KNFLSLADTP NIQCVNSFSK SANGFSHNLN RYDISPAAFI LKSGSPSLQI 250 260 270 280 290 300 HSVEIPWVEP LALADCIQKS GNPICFLDSA KKPGRYSILG ILTGPLARII HYEKATNTTE 310 320 330 340 350 360 IRICKDNSFV RINNDLWSTV ADFMNQHKAI KPDTNLPFYG GIMGIIGYEC SDLSTKSVSN 370 380 390 400 410 420 ASFPLDFQQT TVDAELAFVD RSFVFDLEIK KLFVQTLTPL NETCSEWWGE LLASTCNTKL 430 440 450 460 470 480 DNLSCLHSFD GKQNFGLVQS FPKKEVYCES VKACQEHLLA GDSYEMCLTD TTFVSAPPEL 490 500 510 520 530 540 SDFEMYMRAR SLNPATFAGF VRLNHFTLLC CSPERFLQFR DDRCLFSPIK GTLKREGHMS 550 560 570 580 590 600 LEEARKKLLN EKDMGELNMI IDLIRNDLHQ LAKKNSVHVP ELYSVEEHSN VYSLLSNIYG 610 620 630 640 650 660 RIESPITAWD VLSKSFPPGS MTGAPKLRSV RMLEPLEQHG RGIYSGTLGY WDVTGSAEFN 670 680 690 700 710 VIIRSAFKYK ADDYWRIGAG GAVTILSSPE GEYEEMVLKA NSILPAFVNL KNKKRSCK

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA21814.1.
PIR	T40823.
RefSeq	NP_596536.1.
3D structure databases
HSSP	HSSP built from PDB template 1K0G based on UniProtKB P05041.
ModBase	Search...
Genome annotation databases
GeneID	2541382.
KEGG	spo:SPBP8B7.29.
NMPDR	fig|4896.1.peg.2402.
Organism-specific databases
GeneDB_Spombe	SPBP8B7.29.
Phylogenomic databases
OMA	GESYEIC.
Enzyme and pathway databases
BRENDA	2.6.1.85. 653.
Gene expression databases
ArrayExpress	O94277.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR006220. Anth_synthII. IPR015890. Chorismate-bd_C. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR010117. PabB_fungal. IPR006221. TrpG_papA. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00096. GATASE.
ProDom	PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01823. PabB-fungal. 1 hit. TIGR00566. trpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PABS_SCHPO

Accession

Primary (citable) accession number: O94277

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)