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Protein

25S rRNA (cytosine-C(5))-methyltransferase nop2

Gene

nop2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of a cytosine in 25S rRNA. Required for 60S ribosomal subunit synthesis and processing (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei371 – 3711S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei398 – 3981S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei416 – 4161S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei473 – 4731NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (cytosine-C(5))-methyltransferase nop2 (EC:2.1.1.-)
Alternative name(s):
Nucleolar protein 2
Gene namesi
Name:nop2
ORF Names:SPBP8B7.20c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBP8B7.20c.
PomBaseiSPBP8B7.20c. nop2.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 60860825S rRNA (cytosine-C(5))-methyltransferase nop2PRO_0000317149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphoserine1 Publication
Modified residuei60 – 601Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94268.

PTM databases

iPTMnetiO94268.

Interactioni

Protein-protein interaction databases

BioGridi277788. 7 interactions.
MINTiMINT-4682729.

Structurei

3D structure databases

ProteinModelPortaliO94268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni347 – 3537S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000224258.
InParanoidiO94268.
KOiK14835.
OMAiDEAKECE.
OrthoDBiEOG74FF8R.
PhylomeDBiO94268.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023273. RCMT_NOP2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02012. RCMTNOP2.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKQKSKQG IPPTLEENHN SSHKVTENAK KRKHSKEKPQ NSRKRQLAEE
60 70 80 90 100
KKSLFENSDS ENEKDLIDAD EFEEAETLSD LEHDEEPQTF ADEFIDDEAK
110 120 130 140 150
ECEGEEEDSV FDSDEEHEVK PMFSDDSGDE EDLELANMEA MSRKLDEEAE
160 170 180 190 200
LEEKEAEEEL HTNIHPEAPT VLPPIDGFTD SQPISTLPQD LSQIQLRIQE
210 220 230 240 250
IVRVLNDFKN LCEPGRNRSE YVDQLLNDIC AYYGYSRFLA EKLFELFSVS
260 270 280 290 300
EAVEFFEANE MPRPVTIRTN TLKTQRRELA QALINRGVNL EPIGKWSKVG
310 320 330 340 350
LQVFESQVPI GATPEYLAGH YILQAASSFL PVMALAPQPN ERILDMSSAP
360 370 380 390 400
GGKVTYVAAL QKNTGIIFAN DSNKARTKAL SANIHRLGVR NAIVCNYDGR
410 420 430 440 450
KFPNEVIGGF DRVLLDAPCS GTGVIYKDQS VKTNKSERDF DTLSHLQRQL
460 470 480 490 500
LLSAIDSVNA DSKTGGFIVY STCSITVDED EAVIQYALKK RPNVKLVSTG
510 520 530 540 550
LEFGREGFTR FREKRFHPSL KLTRRYYPHV HNIDGFFVAK LKKISDKIPT
560 570 580 590 600
VNVADDMKDG TNNDVEIEKN STEIDNITFN DEADKEIIEQ NRRKWLKSKG

YKVAKKKD
Length:608
Mass (Da):68,935
Last modified:May 1, 1999 - v1
Checksum:i7930125E2C7D34F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21805.1.
PIRiT40814.
RefSeqiNP_596527.1. NM_001022448.2.

Genome annotation databases

EnsemblFungiiSPBP8B7.20c.1; SPBP8B7.20c.1:pep; SPBP8B7.20c.
GeneIDi2541274.
KEGGispo:SPBP8B7.20c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21805.1.
PIRiT40814.
RefSeqiNP_596527.1. NM_001022448.2.

3D structure databases

ProteinModelPortaliO94268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277788. 7 interactions.
MINTiMINT-4682729.

PTM databases

iPTMnetiO94268.

Proteomic databases

MaxQBiO94268.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBP8B7.20c.1; SPBP8B7.20c.1:pep; SPBP8B7.20c.
GeneIDi2541274.
KEGGispo:SPBP8B7.20c.

Organism-specific databases

EuPathDBiFungiDB:SPBP8B7.20c.
PomBaseiSPBP8B7.20c. nop2.

Phylogenomic databases

HOGENOMiHOG000224258.
InParanoidiO94268.
KOiK14835.
OMAiDEAKECE.
OrthoDBiEOG74FF8R.
PhylomeDBiO94268.

Miscellaneous databases

PROiO94268.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023273. RCMT_NOP2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02012. RCMTNOP2.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58 AND SER-60, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNOP2_SCHPO
AccessioniPrimary (citable) accession number: O94268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.