Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O94266 (THI22_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2

EC=2.7.1.49
EC=2.7.4.7
Alternative name(s):
Hydroxymethylpyrimidine kinase 2
Short name=HMP kinase 2
Hydroxymethylpyrimidine phosphate kinase 2
Short name=HMP-P kinase 2
Short name=HMP-phosphate kinase 2
Short name=HMPP kinase 2
Gene names
ORF Names:SPBP8B7.18c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.

Catalytic activity

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.

ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3.

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

In the N-terminal section; belongs to the ThiD family.

In the C-terminal section; belongs to the thiaminase-2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2
PRO_0000315973

Sites

Binding site761Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O94266 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 8FA207F9F1472C00

FASTA55161,354
        10         20         30         40         50         60 
MPYNPLYESL SNQFDPSRIE TVLDPMGYTI KRRALPVSLT IAGSDCSGGA GIQADLKTMT 

        70         80         90        100        110        120 
SLGVYGMSAI TCLVAENAGG VDSVEEMSPA FVESQIDCCI RDIPCHVVKT GMLGSPEIVK 

       130        140        150        160        170        180 
AVARSAKKFN FSKLVVDPVM VATSGDSLVT KDIVSVLNEE LLPLTYLVTP NIPEAIVLAK 

       190        200        210        220        230        240 
NQGLDISNIN SVSDMERCAA VIHKLGPKHV LLKGGHMPVN NLGLKSSDDE DLRVVDILYD 

       250        260        270        280        290        300 
GNRFYHFSSS YLKKGEVHGT GCTLSSAIAS FLAWEHSLTE AVQFGIDYVH GAITHSPPIN 

       310        320        330        340        350        360 
NCSTNILNHM TRLRIVPFAP GHFIEYILSH PQVVPAWKEY INHKFTNMLA KGTLPLPAFQ 

       370        380        390        400        410        420 
DYLKQDYLYL VNFARAYSLK GYKENTFPNI LEAAQSVIHV IEEKELHVSM CSSYGVSLQD 

       430        440        450        460        470        480 
LKSCEESPAC TAYSRYILDT GAAQDVAALD FVQAPCLIGY YVIAARLMKE PFRNPQGPYQ 

       490        500        510        520        530        540 
KWVDNYFCED YLSAVRRGCR QIEEIVLKLS PERIQELIEI FIRATKFETL FWETPYYEYV 

       550 
TKQNLEDKEF S 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAA21803.1.
PIRT40812.
RefSeqNP_596525.1. NM_001022446.2.

3D structure databases

ProteinModelPortalO94266.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277884. 13 interactions.
MINTMINT-4682707.
STRING4896.SPBP8B7.18c-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBP8B7.18c.1; SPBP8B7.18c.1:pep; SPBP8B7.18c.
GeneID2541373.
KEGGspo:SPBP8B7.18c.

Organism-specific databases

PomBaseSPBP8B7.18c.

Phylogenomic databases

eggNOGCOG0351.
HOGENOMHOG000225275.
KOK00877.
OMAACTAYTR.
OrthoDBEOG75MW5D.
PhylomeDBO94266.

Enzyme and pathway databases

UniPathwayUPA00060; UER00137.
UPA00060; UER00138.

Family and domain databases

Gene3D1.20.910.10. 1 hit.
InterProIPR016084. Haem_Oase-like_multi-hlx.
IPR013749. HMP-P_kinase-1.
IPR004399. HMP-P_kinase-2.
IPR004305. Thiaminase-2/PQQC.
[Graphical view]
PfamPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
[Graphical view]
SUPFAMSSF48613. SSF48613. 1 hit.
TIGRFAMsTIGR00097. HMP-P_kinase. 1 hit.
ProtoNetSearch...

Other

NextBio20802482.

Entry information

Entry nameTHI22_SCHPO
AccessionPrimary (citable) accession number: O94266
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways