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Protein

DNA polymerase epsilon subunit B

Gene

dpb2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase II participates in chromosomal DNA replication.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • DNA biosynthetic process Source: GOC
  • mitotic DNA replication initiation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-174430. Telomere C-strand synthesis initiation.
R-SPO-68952. DNA replication initiation.
R-SPO-68962. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit B (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 2
Gene namesi
Name:dpb2
ORF Names:SPBP8B7.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBP8B7.14c.
PomBaseiSPBP8B7.14c. dpb2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • DNA replication preinitiation complex Source: PomBase
  • epsilon DNA polymerase complex Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 594594DNA polymerase epsilon subunit BPRO_0000071571Add
BLAST

Proteomic databases

MaxQBiO94263.

Interactioni

Subunit structurei

Heterotetramer. Consists of four subunits: pol2, dpb2, dpb3 and dpb4 (By similarity). Interacts with dpb3.By similarity1 Publication

Protein-protein interaction databases

BioGridi277858. 7 interactions.
IntActiO94263. 2 interactions.
MINTiMINT-4682676.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiO94263.
KOiK02325.
OMAiFAFKVYI.
OrthoDBiEOG7XH6ZR.
PhylomeDBiO94263.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
[Graphical view]
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94263-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSITDSVE KKPEQVSFEV QPNALRPVAF HVFTRKYDLN INRDSLQELA
60 70 80 90 100
SFVSKKCGSQ WRSQCEPLLD EIAKTWKRVH ETQPIVTRPL LIPVLANLNV
110 120 130 140 150
PHEVRVSSLA RVQTLETTGS FLNSSNSEIR ETIKNEKYFR VLDAFKMPKF
160 170 180 190 200
KYDSSRKVFV LSKQSPTLMA SASACTDMLN RRFNVVYSRI LRNESFQTPS
210 220 230 240 250
FSGSFSQTGT YQLTPIRNLL GRAGNTFLLF GLLTIAPDGT LWLEDLDSQV
260 270 280 290 300
QLDVSQAEQG FGLFCPGCLV LVNGQFLSSG LFLVFELGHP PIERRDASLK
310 320 330 340 350
ALNNLDILGL NMDAKQLALL RHAEQAYQSQ AFVCISEVHL DNHQTFYALE
360 370 380 390 400
KIFQKYESSE AVPFCIILCG SFMSSAFHNS GSSIQYKEGF NKLAASLEKF
410 420 430 440 450
PKICEKTKFI FVPGPNDPWT TNGISLMPKH SIPLHFVNRI QRVCKHTIFA
460 470 480 490 500
SNPCRIIFFS QEVLIYRDDI SGRFQRNSLK FGKTPQGTSN INSIPLAEQQ
510 520 530 540 550
VHQQRKLVKT VLDQSHLSPF PSRTRPILWD FDYALSVFPL PSCMGLIDSE
560 570 580 590
SSAFDVHYGG CPTFNPGALL LGVHYNWQEV FPVKKEIITH KERI
Length:594
Mass (Da):67,224
Last modified:May 1, 1999 - v1
Checksum:i272CC46AA42ED74A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21799.1.
PIRiT40808.
RefSeqiNP_596521.1. NM_001022442.2.

Genome annotation databases

EnsemblFungiiSPBP8B7.14c.1; SPBP8B7.14c.1:pep; SPBP8B7.14c.
GeneIDi2541347.
KEGGispo:SPBP8B7.14c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21799.1.
PIRiT40808.
RefSeqiNP_596521.1. NM_001022442.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277858. 7 interactions.
IntActiO94263. 2 interactions.
MINTiMINT-4682676.

Proteomic databases

MaxQBiO94263.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBP8B7.14c.1; SPBP8B7.14c.1:pep; SPBP8B7.14c.
GeneIDi2541347.
KEGGispo:SPBP8B7.14c.

Organism-specific databases

EuPathDBiFungiDB:SPBP8B7.14c.
PomBaseiSPBP8B7.14c. dpb2.

Phylogenomic databases

InParanoidiO94263.
KOiK02325.
OMAiFAFKVYI.
OrthoDBiEOG7XH6ZR.
PhylomeDBiO94263.

Enzyme and pathway databases

ReactomeiR-SPO-174430. Telomere C-strand synthesis initiation.
R-SPO-68952. DNA replication initiation.
R-SPO-68962. Activation of the pre-replicative complex.

Miscellaneous databases

PROiO94263.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
[Graphical view]
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast."
    Spiga M.-G., D'Urso G.
    Nucleic Acids Res. 32:4945-4953(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DPB3.

Entry informationi

Entry nameiDPB2_SCHPO
AccessioniPrimary (citable) accession number: O94263
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.