O94235 (MPH1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase mph1
EC=2.7.12.2

Gene names

Name:	mph1
ORF Names:	SPBC106.01, SPBC1271.16c, SPBC243.01

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular protein localization Inferred from mutant phenotype PubMed 11909965. Source: PomBase cellular response to cadmium ion Inferred from mutant phenotype PubMed 18684775. Source: PomBase detoxification of cadmium ion Inferred from mutant phenotype PubMed 18684775. Source: PomBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle assembly checkpoint Inferred from mutant phenotype Ref.1. Source: PomBase signal transduction Non-traceable author statement. Source: PomBase
Cellular_component	condensed nuclear chromosome kinetochore Inferred from sequence orthology. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase spindle pole body Inferred from sequence orthology. Source: PomBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Serine/threonine-protein kinase mph1		PRO_0000086389
Regions
	Domain	316 – 607	292	Protein kinase
	Nucleotide binding	322 – 330	9	ATP By similarity
	Compositional bias	157 – 160	4	Poly-Arg
Sites
	Active site	442	1	Proton acceptor By similarity
	Binding site	345	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94235 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C943950DE001E8E3	FASTA	678	75,354
	10 20 30 40 50 60 MSKRNPPVTN IADLVSDSSL DEDSLSFLEE LQDPELYFKN DTFSSKSSHS DGTVTGDTLR 70 80 90 100 110 120 RQSSGATALE RLVSHPRTKN FDLQGNGGQN SALKEVNTPA YQSMHHFEHL ITPLPSTNAS 130 140 150 160 170 180 HSEVSLSAGV NDLNSNSEHD LLPKSVNKTP GSLSISRRRR IGRIGLGPPK RAEYTLTDPS 190 200 210 220 230 240 KTSDTKNSTE ADEDIEMKSR EVSPASNSVA ATTLKPLQLH NTPLQTSQEH PKPSFHPSQF 250 260 270 280 290 300 ESSFSPRVQF DHDVERRASE LHSRPVTVFQ EPQRSASQPY ESHALSPKVA PLFDNSQATP 310 320 330 340 350 360 IPKRQQDVVT VANLQFIKLG VVGKGGSSMV YRIFSPDNSR LYALKEVNFI NADQTTIQGY 370 380 390 400 410 420 KNEIALLRKL SGNDRIIKLY AAEVNDTLGQ LNMVMECGET DLANLLMKNM KKPINLNFIR 430 440 450 460 470 480 MYWEQMLEAV QVVHDQNIVH SDLKPANFLL VEGNLKLIDF GIAKAIGNDT TNIHRDSHIG 490 500 510 520 530 540 TINYMAPEAL TDMNAHTNSG VKLVKLGRPS DVWSLGCILY QMVYGRAPFA HLKMIQAIAA 550 560 570 580 590 600 IPNEQYHIHF PEVALPANAV QEKEGSLPGV TVGPDLMDVM KRCLERDQRK RLTIPELLVH 610 620 630 640 650 660 PFLNPLPSYL TPLAKKPLPV SGHTNNAHPL RLSTEISASQ LSMIIERSVE LSKHKRLNKE 670 LIDSMAYDCV SNLRKMPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mph1, a member of the Mps1-like family of dual specificity protein kinases, is required for the spindle checkpoint in S. pombe." He X., Jones M.H., Winey M., Sazer S. J. Cell Sci. 111:1635-1647(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020705 mRNA. Translation: AAD01648.1. CU329671 Genomic DNA. Translation: CAB72266.1.
PIR	T43539.
RefSeq	NP_595150.2. NM_001021059.3.
3D structure databases
ProteinModelPortal	O94235.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC106.01-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC106.01.1; SPBC106.01.1:pep; SPBC106.01.
GeneID	2540060.
KEGG	spo:SPBC106.01.
Organism-specific databases
PomBase	SPBC106.01.
Phylogenomic databases
eggNOG	COG0515.
KO	K08866.
OMA	WHANVES.
OrthoDB	EOG45XC4Q.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR027083. Mps1/Mph1. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR22974:SF6. PTHR22974:SF6. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801197.

Entry information

Entry name

MPH1_SCHPO

Accession

Primary (citable) accession number: O94235
Secondary accession number(s): Q9P7Z5, Q9URW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program