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O94230

- IDH2_KLULA

UniProt

O94230 - IDH2_KLULA

Protein

Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial

Gene

IDH2

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Performs an essential role in the oxidative function of the citric acid cycle.By similarity

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181SubstrateBy similarity
    Binding sitei128 – 1281SubstrateBy similarity
    Binding sitei149 – 1491SubstrateBy similarity
    Sitei156 – 1561Critical for catalysisBy similarity
    Sitei203 – 2031Critical for catalysisBy similarity
    Metal bindingi236 – 2361Magnesium or manganeseBy similarity
    Binding sitei236 – 2361SubstrateBy similarity
    Metal bindingi262 – 2621Magnesium or manganeseBy similarity
    Metal bindingi266 – 2661Magnesium or manganeseBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH
    Gene namesi
    Name:IDH2
    Ordered Locus Names:KLLA0E03058g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome E

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1414MitochondrionBy similarityAdd
    BLAST
    Chaini15 – 368354Isocitrate dehydrogenase [NAD] subunit 2, mitochondrialPRO_0000014432Add
    BLAST

    Interactioni

    Subunit structurei

    Octamer of two non-identical subunits IDH1 and IDH2.By similarity

    Protein-protein interaction databases

    STRINGi28985.O94230.

    Structurei

    3D structure databases

    ProteinModelPortaliO94230.
    SMRiO94230. Positions 18-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021113.
    KOiK00030.
    OMAiTPIAWEP.
    OrthoDBiEOG75XGWZ.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O94230-1 [UniParc]FASTAAdd to Basket

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    MFRQSIVKQS CRFLATKKQP SIGRYTGKPN PKTGKYTVSF IEGDGVGPEI    50
    SKSVKAIFSA AKVPIEWESC DVSPIFVNGL TTIPDPAVAS INKNLIALKG 100
    PLATPIGKGH RSLNLTLRKT FGLFANVRPA KSIEGYKTTY ENVNLVLIRE 150
    NTEGEYSGIE HVVAPGVVQS IKLITQDASE RVIRYAFEYA RAVDRSKVLV 200
    VHKSTIQRLA DGLFVDVAKK LSSEYPDIEL QTELLDNTVL KTVQHPEAYD 250
    DVVVVCPNLY GDILSDLNSG LSAGSLGLTP SANIGHTVSI FEAVHGSAPD 300
    IAGQNKANPT ALLLSSVMML NHMGLTEHAD KIEKAVLTTI ASDAKNRTGD 350
    LGGSASTSSF TDAVIERL 368
    Length:368
    Mass (Da):39,579
    Last modified:May 1, 1999 - v1
    Checksum:iE6BFAE7F676F5FB6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF045154 Genomic DNA. Translation: AAC69609.1.
    CR382125 Genomic DNA. Translation: CAG99173.1.
    RefSeqiXP_454086.1. XM_454086.1.

    Genome annotation databases

    GeneIDi2894289.
    KEGGikla:KLLA0E03125g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF045154 Genomic DNA. Translation: AAC69609.1 .
    CR382125 Genomic DNA. Translation: CAG99173.1 .
    RefSeqi XP_454086.1. XM_454086.1.

    3D structure databases

    ProteinModelPortali O94230.
    SMRi O94230. Positions 18-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.O94230.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2894289.
    KEGGi kla:KLLA0E03125g.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021113.
    KOi K00030.
    OMAi TPIAWEP.
    OrthoDBi EOG75XGWZ.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and RNA-binding analysis of NAD+ -isocitrate dehydrogenases from Kluyveromyces lactis and Schizosaccharomyces pombe."
      Elzinga S.D.J., van Oosterum K., Maat C., Grivell L.A., van der Spek H.
      Curr. Genet. 38:87-94(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC MYA-539 / JBD100.
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

    Entry informationi

    Entry nameiIDH2_KLULA
    AccessioniPrimary (citable) accession number: O94230
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3