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Protein

Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial

Gene

IDH2

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Performs an essential role in the oxidative function of the citric acid cycle.By similarity

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei128 – 1281SubstrateBy similarity
Binding sitei149 – 1491SubstrateBy similarity
Sitei156 – 1561Critical for catalysisBy similarity
Sitei203 – 2031Critical for catalysisBy similarity
Metal bindingi236 – 2361Magnesium or manganeseBy similarity
Binding sitei236 – 2361SubstrateBy similarity
Metal bindingi262 – 2621Magnesium or manganeseBy similarity
Metal bindingi266 – 2661Magnesium or manganeseBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase
NAD(+)-specific ICDH
Gene namesi
Name:IDH2
Ordered Locus Names:KLLA0E03058g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome E

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414MitochondrionBy similarityAdd
BLAST
Chaini15 – 368354Isocitrate dehydrogenase [NAD] subunit 2, mitochondrialPRO_0000014432Add
BLAST

Interactioni

Subunit structurei

Octamer of two non-identical subunits IDH1 and IDH2.By similarity

Protein-protein interaction databases

STRINGi28985.O94230.

Structurei

3D structure databases

ProteinModelPortaliO94230.
SMRiO94230. Positions 18-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
InParanoidiO94230.
KOiK00030.
OMAiQITTFAY.
OrthoDBiEOG75XGWZ.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O94230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRQSIVKQS CRFLATKKQP SIGRYTGKPN PKTGKYTVSF IEGDGVGPEI
60 70 80 90 100
SKSVKAIFSA AKVPIEWESC DVSPIFVNGL TTIPDPAVAS INKNLIALKG
110 120 130 140 150
PLATPIGKGH RSLNLTLRKT FGLFANVRPA KSIEGYKTTY ENVNLVLIRE
160 170 180 190 200
NTEGEYSGIE HVVAPGVVQS IKLITQDASE RVIRYAFEYA RAVDRSKVLV
210 220 230 240 250
VHKSTIQRLA DGLFVDVAKK LSSEYPDIEL QTELLDNTVL KTVQHPEAYD
260 270 280 290 300
DVVVVCPNLY GDILSDLNSG LSAGSLGLTP SANIGHTVSI FEAVHGSAPD
310 320 330 340 350
IAGQNKANPT ALLLSSVMML NHMGLTEHAD KIEKAVLTTI ASDAKNRTGD
360
LGGSASTSSF TDAVIERL
Length:368
Mass (Da):39,579
Last modified:May 1, 1999 - v1
Checksum:iE6BFAE7F676F5FB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045154 Genomic DNA. Translation: AAC69609.1.
CR382125 Genomic DNA. Translation: CAG99173.1.
RefSeqiXP_454086.1. XM_454086.1.

Genome annotation databases

GeneIDi2894289.
KEGGikla:KLLA0E03125g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045154 Genomic DNA. Translation: AAC69609.1.
CR382125 Genomic DNA. Translation: CAG99173.1.
RefSeqiXP_454086.1. XM_454086.1.

3D structure databases

ProteinModelPortaliO94230.
SMRiO94230. Positions 18-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.O94230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2894289.
KEGGikla:KLLA0E03125g.

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
InParanoidiO94230.
KOiK00030.
OMAiQITTFAY.
OrthoDBiEOG75XGWZ.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and RNA-binding analysis of NAD+ -isocitrate dehydrogenases from Kluyveromyces lactis and Schizosaccharomyces pombe."
    Elzinga S.D.J., van Oosterum K., Maat C., Grivell L.A., van der Spek H.
    Curr. Genet. 38:87-94(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC MYA-539 / JBD100.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiIDH2_KLULA
AccessioniPrimary (citable) accession number: O94230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.